Cargando…

Uip4p modulates nuclear pore complex function in Saccharomyces cerevisiae

A double membrane bilayer perforated by nuclear pore complexes (NPCs) governs the shape of the nucleus, the prominent distinguishing organelle of a eukaryotic cell. Despite the absence of lamins in yeasts, the nuclear morphology is stably maintained and shape changes occur in a regulated fashion. In...

Descripción completa

Detalles Bibliográficos
Autores principales: Deolal, Pallavi, Jamir, Imlitoshi, Mishra, Krishnaveni
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taylor & Francis 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8855845/
https://www.ncbi.nlm.nih.gov/pubmed/35171083
http://dx.doi.org/10.1080/19491034.2022.2034286
_version_ 1784653727008817152
author Deolal, Pallavi
Jamir, Imlitoshi
Mishra, Krishnaveni
author_facet Deolal, Pallavi
Jamir, Imlitoshi
Mishra, Krishnaveni
author_sort Deolal, Pallavi
collection PubMed
description A double membrane bilayer perforated by nuclear pore complexes (NPCs) governs the shape of the nucleus, the prominent distinguishing organelle of a eukaryotic cell. Despite the absence of lamins in yeasts, the nuclear morphology is stably maintained and shape changes occur in a regulated fashion. In a quest to identify factors that contribute to regulation of nuclear shape and function in Saccharomyces cerevisiae, we used a fluorescence imaging based approach. Here we report the identification of a novel protein, Uip4p, that is required for regulation of nuclear morphology. Loss of Uip4 compromises NPC function and loss of nuclear envelope (NE) integrity. Our localization studies show that Uip4 localizes to the NE and endoplasmic reticulum (ER) network. Furthermore, we demonstrate that the localization and expression of Uip4 is regulated during growth, which is crucial for NPC distribution.
format Online
Article
Text
id pubmed-8855845
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Taylor & Francis
record_format MEDLINE/PubMed
spelling pubmed-88558452022-02-19 Uip4p modulates nuclear pore complex function in Saccharomyces cerevisiae Deolal, Pallavi Jamir, Imlitoshi Mishra, Krishnaveni Nucleus Research Paper A double membrane bilayer perforated by nuclear pore complexes (NPCs) governs the shape of the nucleus, the prominent distinguishing organelle of a eukaryotic cell. Despite the absence of lamins in yeasts, the nuclear morphology is stably maintained and shape changes occur in a regulated fashion. In a quest to identify factors that contribute to regulation of nuclear shape and function in Saccharomyces cerevisiae, we used a fluorescence imaging based approach. Here we report the identification of a novel protein, Uip4p, that is required for regulation of nuclear morphology. Loss of Uip4 compromises NPC function and loss of nuclear envelope (NE) integrity. Our localization studies show that Uip4 localizes to the NE and endoplasmic reticulum (ER) network. Furthermore, we demonstrate that the localization and expression of Uip4 is regulated during growth, which is crucial for NPC distribution. Taylor & Francis 2022-02-16 /pmc/articles/PMC8855845/ /pubmed/35171083 http://dx.doi.org/10.1080/19491034.2022.2034286 Text en © 2022 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Paper
Deolal, Pallavi
Jamir, Imlitoshi
Mishra, Krishnaveni
Uip4p modulates nuclear pore complex function in Saccharomyces cerevisiae
title Uip4p modulates nuclear pore complex function in Saccharomyces cerevisiae
title_full Uip4p modulates nuclear pore complex function in Saccharomyces cerevisiae
title_fullStr Uip4p modulates nuclear pore complex function in Saccharomyces cerevisiae
title_full_unstemmed Uip4p modulates nuclear pore complex function in Saccharomyces cerevisiae
title_short Uip4p modulates nuclear pore complex function in Saccharomyces cerevisiae
title_sort uip4p modulates nuclear pore complex function in saccharomyces cerevisiae
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8855845/
https://www.ncbi.nlm.nih.gov/pubmed/35171083
http://dx.doi.org/10.1080/19491034.2022.2034286
work_keys_str_mv AT deolalpallavi uip4pmodulatesnuclearporecomplexfunctioninsaccharomycescerevisiae
AT jamirimlitoshi uip4pmodulatesnuclearporecomplexfunctioninsaccharomycescerevisiae
AT mishrakrishnaveni uip4pmodulatesnuclearporecomplexfunctioninsaccharomycescerevisiae