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Uip4p modulates nuclear pore complex function in Saccharomyces cerevisiae
A double membrane bilayer perforated by nuclear pore complexes (NPCs) governs the shape of the nucleus, the prominent distinguishing organelle of a eukaryotic cell. Despite the absence of lamins in yeasts, the nuclear morphology is stably maintained and shape changes occur in a regulated fashion. In...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Taylor & Francis
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8855845/ https://www.ncbi.nlm.nih.gov/pubmed/35171083 http://dx.doi.org/10.1080/19491034.2022.2034286 |
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author | Deolal, Pallavi Jamir, Imlitoshi Mishra, Krishnaveni |
author_facet | Deolal, Pallavi Jamir, Imlitoshi Mishra, Krishnaveni |
author_sort | Deolal, Pallavi |
collection | PubMed |
description | A double membrane bilayer perforated by nuclear pore complexes (NPCs) governs the shape of the nucleus, the prominent distinguishing organelle of a eukaryotic cell. Despite the absence of lamins in yeasts, the nuclear morphology is stably maintained and shape changes occur in a regulated fashion. In a quest to identify factors that contribute to regulation of nuclear shape and function in Saccharomyces cerevisiae, we used a fluorescence imaging based approach. Here we report the identification of a novel protein, Uip4p, that is required for regulation of nuclear morphology. Loss of Uip4 compromises NPC function and loss of nuclear envelope (NE) integrity. Our localization studies show that Uip4 localizes to the NE and endoplasmic reticulum (ER) network. Furthermore, we demonstrate that the localization and expression of Uip4 is regulated during growth, which is crucial for NPC distribution. |
format | Online Article Text |
id | pubmed-8855845 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Taylor & Francis |
record_format | MEDLINE/PubMed |
spelling | pubmed-88558452022-02-19 Uip4p modulates nuclear pore complex function in Saccharomyces cerevisiae Deolal, Pallavi Jamir, Imlitoshi Mishra, Krishnaveni Nucleus Research Paper A double membrane bilayer perforated by nuclear pore complexes (NPCs) governs the shape of the nucleus, the prominent distinguishing organelle of a eukaryotic cell. Despite the absence of lamins in yeasts, the nuclear morphology is stably maintained and shape changes occur in a regulated fashion. In a quest to identify factors that contribute to regulation of nuclear shape and function in Saccharomyces cerevisiae, we used a fluorescence imaging based approach. Here we report the identification of a novel protein, Uip4p, that is required for regulation of nuclear morphology. Loss of Uip4 compromises NPC function and loss of nuclear envelope (NE) integrity. Our localization studies show that Uip4 localizes to the NE and endoplasmic reticulum (ER) network. Furthermore, we demonstrate that the localization and expression of Uip4 is regulated during growth, which is crucial for NPC distribution. Taylor & Francis 2022-02-16 /pmc/articles/PMC8855845/ /pubmed/35171083 http://dx.doi.org/10.1080/19491034.2022.2034286 Text en © 2022 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Paper Deolal, Pallavi Jamir, Imlitoshi Mishra, Krishnaveni Uip4p modulates nuclear pore complex function in Saccharomyces cerevisiae |
title | Uip4p modulates nuclear pore complex function in Saccharomyces cerevisiae |
title_full | Uip4p modulates nuclear pore complex function in Saccharomyces cerevisiae |
title_fullStr | Uip4p modulates nuclear pore complex function in Saccharomyces cerevisiae |
title_full_unstemmed | Uip4p modulates nuclear pore complex function in Saccharomyces cerevisiae |
title_short | Uip4p modulates nuclear pore complex function in Saccharomyces cerevisiae |
title_sort | uip4p modulates nuclear pore complex function in saccharomyces cerevisiae |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8855845/ https://www.ncbi.nlm.nih.gov/pubmed/35171083 http://dx.doi.org/10.1080/19491034.2022.2034286 |
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