Evaluation of the Cepheid Xpert Xpress SARS-CoV-2 test for bronchoalveolar lavage

Accurate and rapid laboratory tests are essential for the prompt diagnosis of COVID-19, which is important to patients and infection control. The Xpert Xpress SARS-CoV-2 test is a real-time RT-PCR intended for the qualitative detection of nucleic acid from SARS-CoV-2 in upper respiratory specimens....

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Detalles Bibliográficos
Autores principales: Phan, Tung, Mays, Ashley, McCullough, Melissa, Wells, Alan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Author(s). Published by Elsevier Ltd. 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8856763/
https://www.ncbi.nlm.nih.gov/pubmed/35262037
http://dx.doi.org/10.1016/j.jcvp.2022.100067
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author Phan, Tung
Mays, Ashley
McCullough, Melissa
Wells, Alan
author_facet Phan, Tung
Mays, Ashley
McCullough, Melissa
Wells, Alan
author_sort Phan, Tung
collection PubMed
description Accurate and rapid laboratory tests are essential for the prompt diagnosis of COVID-19, which is important to patients and infection control. The Xpert Xpress SARS-CoV-2 test is a real-time RT-PCR intended for the qualitative detection of nucleic acid from SARS-CoV-2 in upper respiratory specimens. In this study, we assessed the analytical performance characteristics of this rapid test for SARS-CoV-2 in 60 bronchoalveolar lavage (BAL) specimens. BAL is a specimen type that is not authorized under EUA for the Xpert Xpress SARS-CoV-2 test. The limit of detection of the Xpert Xpress SARS-CoV-2 test was 500 copies/ml. The overall agreement of the Xpert Xpress SARS-CoV-2 test was 100%. The Xpert Xpress SARS-CoV-2 test is sensitive and specific to aid in diagnosis of COVID-19 using bronchoalveolar lavage.
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spelling pubmed-88567632022-02-22 Evaluation of the Cepheid Xpert Xpress SARS-CoV-2 test for bronchoalveolar lavage Phan, Tung Mays, Ashley McCullough, Melissa Wells, Alan J Clin Virol Plus Article Accurate and rapid laboratory tests are essential for the prompt diagnosis of COVID-19, which is important to patients and infection control. The Xpert Xpress SARS-CoV-2 test is a real-time RT-PCR intended for the qualitative detection of nucleic acid from SARS-CoV-2 in upper respiratory specimens. In this study, we assessed the analytical performance characteristics of this rapid test for SARS-CoV-2 in 60 bronchoalveolar lavage (BAL) specimens. BAL is a specimen type that is not authorized under EUA for the Xpert Xpress SARS-CoV-2 test. The limit of detection of the Xpert Xpress SARS-CoV-2 test was 500 copies/ml. The overall agreement of the Xpert Xpress SARS-CoV-2 test was 100%. The Xpert Xpress SARS-CoV-2 test is sensitive and specific to aid in diagnosis of COVID-19 using bronchoalveolar lavage. The Author(s). Published by Elsevier Ltd. 2022-06 2022-02-19 /pmc/articles/PMC8856763/ /pubmed/35262037 http://dx.doi.org/10.1016/j.jcvp.2022.100067 Text en © 2022 The Author(s) Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Article
Phan, Tung
Mays, Ashley
McCullough, Melissa
Wells, Alan
Evaluation of the Cepheid Xpert Xpress SARS-CoV-2 test for bronchoalveolar lavage
title Evaluation of the Cepheid Xpert Xpress SARS-CoV-2 test for bronchoalveolar lavage
title_full Evaluation of the Cepheid Xpert Xpress SARS-CoV-2 test for bronchoalveolar lavage
title_fullStr Evaluation of the Cepheid Xpert Xpress SARS-CoV-2 test for bronchoalveolar lavage
title_full_unstemmed Evaluation of the Cepheid Xpert Xpress SARS-CoV-2 test for bronchoalveolar lavage
title_short Evaluation of the Cepheid Xpert Xpress SARS-CoV-2 test for bronchoalveolar lavage
title_sort evaluation of the cepheid xpert xpress sars-cov-2 test for bronchoalveolar lavage
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8856763/
https://www.ncbi.nlm.nih.gov/pubmed/35262037
http://dx.doi.org/10.1016/j.jcvp.2022.100067
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