Co-delivery of genes can be confounded by bicistronic vector design

Maximizing the efficiency of nanocarrier-mediated co-delivery of genes for co-expression in the same cell is critical for many applications. Strategies to maximize co-delivery of nucleic acids (NA) focused largely on carrier systems, with little attention towards payload composition itself. Here, we...

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Detalles Bibliográficos
Autores principales: Moradian, Hanieh, Gossen, Manfred, Lendlein, Andreas
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer International Publishing 2022
Materias:
Research Letter
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8856875/
https://www.ncbi.nlm.nih.gov/pubmed/35223145
http://dx.doi.org/10.1557/s43579-021-00128-7
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author Moradian, Hanieh
Gossen, Manfred
Lendlein, Andreas
author_facet Moradian, Hanieh
Gossen, Manfred
Lendlein, Andreas
author_sort Moradian, Hanieh
collection PubMed
description Maximizing the efficiency of nanocarrier-mediated co-delivery of genes for co-expression in the same cell is critical for many applications. Strategies to maximize co-delivery of nucleic acids (NA) focused largely on carrier systems, with little attention towards payload composition itself. Here, we investigated the effects of different payload designs: co-delivery of two individual “monocistronic” NAs versus a single bicistronic NA comprising two genes separated by a 2A self-cleavage site. Unexpectedly, co-delivery via the monocistronic design resulted in a higher percentage of co-expressing cells, while predictive co-expression via the bicistronic design remained elusive. Our results will aid the application-dependent selection of the optimal methodology for co-delivery of genes. GRAPHICAL ABSTRACT: [Image: see text] SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1557/s43579-021-00128-7.
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spelling pubmed-88568752022-02-22 Co-delivery of genes can be confounded by bicistronic vector design Moradian, Hanieh Gossen, Manfred Lendlein, Andreas MRS Commun Research Letter Maximizing the efficiency of nanocarrier-mediated co-delivery of genes for co-expression in the same cell is critical for many applications. Strategies to maximize co-delivery of nucleic acids (NA) focused largely on carrier systems, with little attention towards payload composition itself. Here, we investigated the effects of different payload designs: co-delivery of two individual “monocistronic” NAs versus a single bicistronic NA comprising two genes separated by a 2A self-cleavage site. Unexpectedly, co-delivery via the monocistronic design resulted in a higher percentage of co-expressing cells, while predictive co-expression via the bicistronic design remained elusive. Our results will aid the application-dependent selection of the optimal methodology for co-delivery of genes. GRAPHICAL ABSTRACT: [Image: see text] SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1557/s43579-021-00128-7. Springer International Publishing 2022-02-18 2022 /pmc/articles/PMC8856875/ /pubmed/35223145 http://dx.doi.org/10.1557/s43579-021-00128-7 Text en © Helmholtz-Zentrum Hereon 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Research Letter
Moradian, Hanieh
Gossen, Manfred
Lendlein, Andreas
Co-delivery of genes can be confounded by bicistronic vector design
title Co-delivery of genes can be confounded by bicistronic vector design
title_full Co-delivery of genes can be confounded by bicistronic vector design
title_fullStr Co-delivery of genes can be confounded by bicistronic vector design
title_full_unstemmed Co-delivery of genes can be confounded by bicistronic vector design
title_short Co-delivery of genes can be confounded by bicistronic vector design
title_sort co-delivery of genes can be confounded by bicistronic vector design
topic Research Letter
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8856875/
https://www.ncbi.nlm.nih.gov/pubmed/35223145
http://dx.doi.org/10.1557/s43579-021-00128-7
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