Cargando…

Comparative cytogenetics on eight Malagasy Mantellinae (Anura, Mantellidae) and a synthesis of the karyological data on the subfamily

We performed a molecular and cytogenetic analysis on different Mantellinae species and revised the available chromosomal data on this group to provide an updated assessment of its karyological diversity and evolution. Using a fragment of the mitochondrial 16S rRNA, we performed a molecular taxonomic...

Descripción completa

Detalles Bibliográficos
Autores principales: Mezzasalma, Marcello, Andreone, Franco, Odierna, Gaetano, Guarino, Fabio Maria, Crottini, Angelica
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Pensoft Publishers 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8857137/
https://www.ncbi.nlm.nih.gov/pubmed/35211250
http://dx.doi.org/10.3897/compcytogen.v16.i1.76260
_version_ 1784653978976387072
author Mezzasalma, Marcello
Andreone, Franco
Odierna, Gaetano
Guarino, Fabio Maria
Crottini, Angelica
author_facet Mezzasalma, Marcello
Andreone, Franco
Odierna, Gaetano
Guarino, Fabio Maria
Crottini, Angelica
author_sort Mezzasalma, Marcello
collection PubMed
description We performed a molecular and cytogenetic analysis on different Mantellinae species and revised the available chromosomal data on this group to provide an updated assessment of its karyological diversity and evolution. Using a fragment of the mitochondrial 16S rRNA, we performed a molecular taxonomic identification of the samples that were used for cytogenetic analyses. A comparative cytogenetic analysis, with Giemsa’s staining, Ag-NOR staining and sequential C-banding + Giemsa + CMA + DAPI was performed on eight species: Gephyromantis sp. Ca19, G.striatus (Vences, Glaw, Andreone, Jesu et Schimmenti, 2002), Mantidactylus (Chonomantis) sp. Ca11, M. (Brygoomantis) alutus (Peracca, 1893), M. (Hylobatrachus) cowanii (Boulenger, 1882), Spinomantispropeaglavei “North” (Methuen et Hewitt, 1913), S.phantasticus (Glaw et Vences, 1997) and S. sp. Ca3. Gephyromantisstriatus, M. (Brygoomantis) alutus and Spinomantispropeaglavei “North” have a karyotype of 2n = 24 chromosomes while the other species show 2n = 26 chromosomes. Among the analysed species we detected differences in the number and position of telocentric elements, location of NOR loci (alternatively on the 6(th), 7(th) or 10(th) pair) and in the distribution of heterochromatin, which shows species-specific patterns. Merging our data with those previously available, we propose a karyotype of 2n = 26 with all biarmed elements and loci of NORs on the 6(th) chromosome pair as the ancestral state in the whole family Mantellidae. From this putative ancestral condition, a reduction of chromosome number through similar tandem fusions (from 2n = 26 to 2n = 24) occurred independently in Mantidactylus Boulenger, 1895 (subgenus Brygoomantis Dubois, 1992), Spinomantis Dubois, 1992 and Gephyromantis Methuen, 1920. Similarly, a relocation of NORs, from the putative primitive configuration on the 6(th) chromosome, occurred independently in Gephyromantis, Blommersia Dubois, 1992, Guibemantis Dubois, 1992, Mantella Boulenger, 1882 and Spinomantis. Chromosome inversions of primitive biarmed elements likely generated a variable number of telocentric elements in Mantellanigricans Guibé, 1978 and a different number of taxa of Gephyromantis (subgenera Duboimantis Glaw et Vences, 2006 and Laurentomantis Dubois, 1980) and Mantidactylus (subgenera Brygoomantis, Chonomantis Glaw et Vences, 1994, Hylobatrachus Laurent, 1943 and Ochthomantis Glaw et Vences, 1994).
format Online
Article
Text
id pubmed-8857137
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Pensoft Publishers
record_format MEDLINE/PubMed
spelling pubmed-88571372022-02-23 Comparative cytogenetics on eight Malagasy Mantellinae (Anura, Mantellidae) and a synthesis of the karyological data on the subfamily Mezzasalma, Marcello Andreone, Franco Odierna, Gaetano Guarino, Fabio Maria Crottini, Angelica Comp Cytogenet Research Article We performed a molecular and cytogenetic analysis on different Mantellinae species and revised the available chromosomal data on this group to provide an updated assessment of its karyological diversity and evolution. Using a fragment of the mitochondrial 16S rRNA, we performed a molecular taxonomic identification of the samples that were used for cytogenetic analyses. A comparative cytogenetic analysis, with Giemsa’s staining, Ag-NOR staining and sequential C-banding + Giemsa + CMA + DAPI was performed on eight species: Gephyromantis sp. Ca19, G.striatus (Vences, Glaw, Andreone, Jesu et Schimmenti, 2002), Mantidactylus (Chonomantis) sp. Ca11, M. (Brygoomantis) alutus (Peracca, 1893), M. (Hylobatrachus) cowanii (Boulenger, 1882), Spinomantispropeaglavei “North” (Methuen et Hewitt, 1913), S.phantasticus (Glaw et Vences, 1997) and S. sp. Ca3. Gephyromantisstriatus, M. (Brygoomantis) alutus and Spinomantispropeaglavei “North” have a karyotype of 2n = 24 chromosomes while the other species show 2n = 26 chromosomes. Among the analysed species we detected differences in the number and position of telocentric elements, location of NOR loci (alternatively on the 6(th), 7(th) or 10(th) pair) and in the distribution of heterochromatin, which shows species-specific patterns. Merging our data with those previously available, we propose a karyotype of 2n = 26 with all biarmed elements and loci of NORs on the 6(th) chromosome pair as the ancestral state in the whole family Mantellidae. From this putative ancestral condition, a reduction of chromosome number through similar tandem fusions (from 2n = 26 to 2n = 24) occurred independently in Mantidactylus Boulenger, 1895 (subgenus Brygoomantis Dubois, 1992), Spinomantis Dubois, 1992 and Gephyromantis Methuen, 1920. Similarly, a relocation of NORs, from the putative primitive configuration on the 6(th) chromosome, occurred independently in Gephyromantis, Blommersia Dubois, 1992, Guibemantis Dubois, 1992, Mantella Boulenger, 1882 and Spinomantis. Chromosome inversions of primitive biarmed elements likely generated a variable number of telocentric elements in Mantellanigricans Guibé, 1978 and a different number of taxa of Gephyromantis (subgenera Duboimantis Glaw et Vences, 2006 and Laurentomantis Dubois, 1980) and Mantidactylus (subgenera Brygoomantis, Chonomantis Glaw et Vences, 1994, Hylobatrachus Laurent, 1943 and Ochthomantis Glaw et Vences, 1994). Pensoft Publishers 2022-02-11 /pmc/articles/PMC8857137/ /pubmed/35211250 http://dx.doi.org/10.3897/compcytogen.v16.i1.76260 Text en Marcello Mezzasalma, Franco Andreone, Gaetano Odierna, Fabio Maria Guarino, Angelica Crottini https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Mezzasalma, Marcello
Andreone, Franco
Odierna, Gaetano
Guarino, Fabio Maria
Crottini, Angelica
Comparative cytogenetics on eight Malagasy Mantellinae (Anura, Mantellidae) and a synthesis of the karyological data on the subfamily
title Comparative cytogenetics on eight Malagasy Mantellinae (Anura, Mantellidae) and a synthesis of the karyological data on the subfamily
title_full Comparative cytogenetics on eight Malagasy Mantellinae (Anura, Mantellidae) and a synthesis of the karyological data on the subfamily
title_fullStr Comparative cytogenetics on eight Malagasy Mantellinae (Anura, Mantellidae) and a synthesis of the karyological data on the subfamily
title_full_unstemmed Comparative cytogenetics on eight Malagasy Mantellinae (Anura, Mantellidae) and a synthesis of the karyological data on the subfamily
title_short Comparative cytogenetics on eight Malagasy Mantellinae (Anura, Mantellidae) and a synthesis of the karyological data on the subfamily
title_sort comparative cytogenetics on eight malagasy mantellinae (anura, mantellidae) and a synthesis of the karyological data on the subfamily
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8857137/
https://www.ncbi.nlm.nih.gov/pubmed/35211250
http://dx.doi.org/10.3897/compcytogen.v16.i1.76260
work_keys_str_mv AT mezzasalmamarcello comparativecytogeneticsoneightmalagasymantellinaeanuramantellidaeandasynthesisofthekaryologicaldataonthesubfamily
AT andreonefranco comparativecytogeneticsoneightmalagasymantellinaeanuramantellidaeandasynthesisofthekaryologicaldataonthesubfamily
AT odiernagaetano comparativecytogeneticsoneightmalagasymantellinaeanuramantellidaeandasynthesisofthekaryologicaldataonthesubfamily
AT guarinofabiomaria comparativecytogeneticsoneightmalagasymantellinaeanuramantellidaeandasynthesisofthekaryologicaldataonthesubfamily
AT crottiniangelica comparativecytogeneticsoneightmalagasymantellinaeanuramantellidaeandasynthesisofthekaryologicaldataonthesubfamily