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Performances of rapid and connected salivary RT-LAMP diagnostic test for SARS-CoV-2 infection in ambulatory screening

In the context of social events reopening and economic relaunch, sanitary surveillance of SARS-CoV-2 infection is still required. Here, we evaluated the diagnostic performances of a rapid, extraction-free and connected reverse-transcription loop-mediated isothermal amplification (RT-LAMP) assay on s...

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Autores principales: Schneider, Francisco Santos, Molina, Laurence, Picot, Marie-Christine, L’Helgoualch, Nicolas, Espeut, Julien, Champigneux, Pierre, Alali, Mellis, Baptiste, Julie, Cardeur, Lise, Carniel, Christophe, Davy, Martin, Dedisse, Daniel, Dubuc, Benjamin, Fenech, Hugo, Foulongne, Vincent, Gaillard, Carole Fruchart, Galtier, Florence, Makinson, Alain, Marin, Grégory, Santos, Raissa Medina, Morquin, David, Ouedraogo, Alimata, Lejeune, Alexandra Prieux, Quenot, Marine, Keiflin, Pierre, Robles, Francisco Checa, Rego, Carolina Rodrigues, Salvetat, Nicolas, Trento, Charline, Vetter, Diana, Molina, Franck, Reynes, Jacques
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8857239/
https://www.ncbi.nlm.nih.gov/pubmed/35181680
http://dx.doi.org/10.1038/s41598-022-04826-7
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author Schneider, Francisco Santos
Molina, Laurence
Picot, Marie-Christine
L’Helgoualch, Nicolas
Espeut, Julien
Champigneux, Pierre
Alali, Mellis
Baptiste, Julie
Cardeur, Lise
Carniel, Christophe
Davy, Martin
Dedisse, Daniel
Dubuc, Benjamin
Fenech, Hugo
Foulongne, Vincent
Gaillard, Carole Fruchart
Galtier, Florence
Makinson, Alain
Marin, Grégory
Santos, Raissa Medina
Morquin, David
Ouedraogo, Alimata
Lejeune, Alexandra Prieux
Quenot, Marine
Keiflin, Pierre
Robles, Francisco Checa
Rego, Carolina Rodrigues
Salvetat, Nicolas
Trento, Charline
Vetter, Diana
Molina, Franck
Reynes, Jacques
author_facet Schneider, Francisco Santos
Molina, Laurence
Picot, Marie-Christine
L’Helgoualch, Nicolas
Espeut, Julien
Champigneux, Pierre
Alali, Mellis
Baptiste, Julie
Cardeur, Lise
Carniel, Christophe
Davy, Martin
Dedisse, Daniel
Dubuc, Benjamin
Fenech, Hugo
Foulongne, Vincent
Gaillard, Carole Fruchart
Galtier, Florence
Makinson, Alain
Marin, Grégory
Santos, Raissa Medina
Morquin, David
Ouedraogo, Alimata
Lejeune, Alexandra Prieux
Quenot, Marine
Keiflin, Pierre
Robles, Francisco Checa
Rego, Carolina Rodrigues
Salvetat, Nicolas
Trento, Charline
Vetter, Diana
Molina, Franck
Reynes, Jacques
author_sort Schneider, Francisco Santos
collection PubMed
description In the context of social events reopening and economic relaunch, sanitary surveillance of SARS-CoV-2 infection is still required. Here, we evaluated the diagnostic performances of a rapid, extraction-free and connected reverse-transcription loop-mediated isothermal amplification (RT-LAMP) assay on saliva. Nasopharyngeal (NP) swabs and saliva from 443 outpatients were collected simultaneously and tested by reverse-transcription quantitative PCR (RT-qPCR) as reference standard test. Seventy-one individuals (16.0%) were positive by NP and/or salivary RT-qPCR. Sensitivity and specificity of salivary RT-LAMP were 85.9% (95%CI 77.8–94.0%) and 99.5% (98.7–100%), respectively. Performances were similar for symptomatic and asymptomatic participants. Moreover, SARS-CoV-2 genetic variants were analyzed and no dominant mutation in RT-LAMP primer region was observed during the period of the study. We demonstrated that this RT-LAMP test on self-collected saliva is reliable for SARS-CoV-2 detection. This simple connected test with optional automatic results transfer to health authorities is unique and opens the way to secure professional and social events in actual context of economics restart.
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spelling pubmed-88572392022-02-22 Performances of rapid and connected salivary RT-LAMP diagnostic test for SARS-CoV-2 infection in ambulatory screening Schneider, Francisco Santos Molina, Laurence Picot, Marie-Christine L’Helgoualch, Nicolas Espeut, Julien Champigneux, Pierre Alali, Mellis Baptiste, Julie Cardeur, Lise Carniel, Christophe Davy, Martin Dedisse, Daniel Dubuc, Benjamin Fenech, Hugo Foulongne, Vincent Gaillard, Carole Fruchart Galtier, Florence Makinson, Alain Marin, Grégory Santos, Raissa Medina Morquin, David Ouedraogo, Alimata Lejeune, Alexandra Prieux Quenot, Marine Keiflin, Pierre Robles, Francisco Checa Rego, Carolina Rodrigues Salvetat, Nicolas Trento, Charline Vetter, Diana Molina, Franck Reynes, Jacques Sci Rep Article In the context of social events reopening and economic relaunch, sanitary surveillance of SARS-CoV-2 infection is still required. Here, we evaluated the diagnostic performances of a rapid, extraction-free and connected reverse-transcription loop-mediated isothermal amplification (RT-LAMP) assay on saliva. Nasopharyngeal (NP) swabs and saliva from 443 outpatients were collected simultaneously and tested by reverse-transcription quantitative PCR (RT-qPCR) as reference standard test. Seventy-one individuals (16.0%) were positive by NP and/or salivary RT-qPCR. Sensitivity and specificity of salivary RT-LAMP were 85.9% (95%CI 77.8–94.0%) and 99.5% (98.7–100%), respectively. Performances were similar for symptomatic and asymptomatic participants. Moreover, SARS-CoV-2 genetic variants were analyzed and no dominant mutation in RT-LAMP primer region was observed during the period of the study. We demonstrated that this RT-LAMP test on self-collected saliva is reliable for SARS-CoV-2 detection. This simple connected test with optional automatic results transfer to health authorities is unique and opens the way to secure professional and social events in actual context of economics restart. Nature Publishing Group UK 2022-02-18 /pmc/articles/PMC8857239/ /pubmed/35181680 http://dx.doi.org/10.1038/s41598-022-04826-7 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Schneider, Francisco Santos
Molina, Laurence
Picot, Marie-Christine
L’Helgoualch, Nicolas
Espeut, Julien
Champigneux, Pierre
Alali, Mellis
Baptiste, Julie
Cardeur, Lise
Carniel, Christophe
Davy, Martin
Dedisse, Daniel
Dubuc, Benjamin
Fenech, Hugo
Foulongne, Vincent
Gaillard, Carole Fruchart
Galtier, Florence
Makinson, Alain
Marin, Grégory
Santos, Raissa Medina
Morquin, David
Ouedraogo, Alimata
Lejeune, Alexandra Prieux
Quenot, Marine
Keiflin, Pierre
Robles, Francisco Checa
Rego, Carolina Rodrigues
Salvetat, Nicolas
Trento, Charline
Vetter, Diana
Molina, Franck
Reynes, Jacques
Performances of rapid and connected salivary RT-LAMP diagnostic test for SARS-CoV-2 infection in ambulatory screening
title Performances of rapid and connected salivary RT-LAMP diagnostic test for SARS-CoV-2 infection in ambulatory screening
title_full Performances of rapid and connected salivary RT-LAMP diagnostic test for SARS-CoV-2 infection in ambulatory screening
title_fullStr Performances of rapid and connected salivary RT-LAMP diagnostic test for SARS-CoV-2 infection in ambulatory screening
title_full_unstemmed Performances of rapid and connected salivary RT-LAMP diagnostic test for SARS-CoV-2 infection in ambulatory screening
title_short Performances of rapid and connected salivary RT-LAMP diagnostic test for SARS-CoV-2 infection in ambulatory screening
title_sort performances of rapid and connected salivary rt-lamp diagnostic test for sars-cov-2 infection in ambulatory screening
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8857239/
https://www.ncbi.nlm.nih.gov/pubmed/35181680
http://dx.doi.org/10.1038/s41598-022-04826-7
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