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MqsR toxin as a biotechnological tool for plant pathogen bacterial control
Type II toxin-antitoxin (TA) systems are widespread in bacteria and are involved in important cell features, such as cell growth inhibition and antimicrobial tolerance, through the induction of persister cells. Overall, these characteristics are associated with bacterial survival under stress condit...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Nature Publishing Group UK
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8857320/ https://www.ncbi.nlm.nih.gov/pubmed/35181693 http://dx.doi.org/10.1038/s41598-022-06690-x |
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author | de Souza-Neto, Reinaldo Rodrigues Carvalho, Isis Gabriela Barbosa Martins, Paula Maria Moreira Picchi, Simone Cristina Tomaz, Juarez Pires Caserta, Raquel Takita, Marco Aurélio de Souza, Alessandra Alves |
author_facet | de Souza-Neto, Reinaldo Rodrigues Carvalho, Isis Gabriela Barbosa Martins, Paula Maria Moreira Picchi, Simone Cristina Tomaz, Juarez Pires Caserta, Raquel Takita, Marco Aurélio de Souza, Alessandra Alves |
author_sort | de Souza-Neto, Reinaldo Rodrigues |
collection | PubMed |
description | Type II toxin-antitoxin (TA) systems are widespread in bacteria and are involved in important cell features, such as cell growth inhibition and antimicrobial tolerance, through the induction of persister cells. Overall, these characteristics are associated with bacterial survival under stress conditions and represent a significant genetic mechanism to be explored for antibacterial molecules. We verified that even though Xylella fastidiosa and Xanthomonas citri subsp. citri share closely related genomes, they have different Type II TA system contents. One important difference is the absence of mqsRA in X. citri. The toxin component of this TA system has been shown to inhibit the growth of X. fastidiosa. Thus, the absence of mqsRA in X. citri led us to explore the possibility of using the MqsR toxin to impair X. citri growth. We purified MqsR and confirmed that the toxin was able to inhibit X. citri. Subsequently, transgenic citrus plants producing MqsR showed a significant reduction in citrus canker and citrus variegated chlorosis symptoms caused, respectively, by X. citri and X. fastidiosa. This study demonstrates that the use of toxins from TA systems is a promising strategy to be explored aiming bacterial control. |
format | Online Article Text |
id | pubmed-8857320 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-88573202022-02-22 MqsR toxin as a biotechnological tool for plant pathogen bacterial control de Souza-Neto, Reinaldo Rodrigues Carvalho, Isis Gabriela Barbosa Martins, Paula Maria Moreira Picchi, Simone Cristina Tomaz, Juarez Pires Caserta, Raquel Takita, Marco Aurélio de Souza, Alessandra Alves Sci Rep Article Type II toxin-antitoxin (TA) systems are widespread in bacteria and are involved in important cell features, such as cell growth inhibition and antimicrobial tolerance, through the induction of persister cells. Overall, these characteristics are associated with bacterial survival under stress conditions and represent a significant genetic mechanism to be explored for antibacterial molecules. We verified that even though Xylella fastidiosa and Xanthomonas citri subsp. citri share closely related genomes, they have different Type II TA system contents. One important difference is the absence of mqsRA in X. citri. The toxin component of this TA system has been shown to inhibit the growth of X. fastidiosa. Thus, the absence of mqsRA in X. citri led us to explore the possibility of using the MqsR toxin to impair X. citri growth. We purified MqsR and confirmed that the toxin was able to inhibit X. citri. Subsequently, transgenic citrus plants producing MqsR showed a significant reduction in citrus canker and citrus variegated chlorosis symptoms caused, respectively, by X. citri and X. fastidiosa. This study demonstrates that the use of toxins from TA systems is a promising strategy to be explored aiming bacterial control. Nature Publishing Group UK 2022-02-18 /pmc/articles/PMC8857320/ /pubmed/35181693 http://dx.doi.org/10.1038/s41598-022-06690-x Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article de Souza-Neto, Reinaldo Rodrigues Carvalho, Isis Gabriela Barbosa Martins, Paula Maria Moreira Picchi, Simone Cristina Tomaz, Juarez Pires Caserta, Raquel Takita, Marco Aurélio de Souza, Alessandra Alves MqsR toxin as a biotechnological tool for plant pathogen bacterial control |
title | MqsR toxin as a biotechnological tool for plant pathogen bacterial control |
title_full | MqsR toxin as a biotechnological tool for plant pathogen bacterial control |
title_fullStr | MqsR toxin as a biotechnological tool for plant pathogen bacterial control |
title_full_unstemmed | MqsR toxin as a biotechnological tool for plant pathogen bacterial control |
title_short | MqsR toxin as a biotechnological tool for plant pathogen bacterial control |
title_sort | mqsr toxin as a biotechnological tool for plant pathogen bacterial control |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8857320/ https://www.ncbi.nlm.nih.gov/pubmed/35181693 http://dx.doi.org/10.1038/s41598-022-06690-x |
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