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MqsR toxin as a biotechnological tool for plant pathogen bacterial control

Type II toxin-antitoxin (TA) systems are widespread in bacteria and are involved in important cell features, such as cell growth inhibition and antimicrobial tolerance, through the induction of persister cells. Overall, these characteristics are associated with bacterial survival under stress condit...

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Autores principales: de Souza-Neto, Reinaldo Rodrigues, Carvalho, Isis Gabriela Barbosa, Martins, Paula Maria Moreira, Picchi, Simone Cristina, Tomaz, Juarez Pires, Caserta, Raquel, Takita, Marco Aurélio, de Souza, Alessandra Alves
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8857320/
https://www.ncbi.nlm.nih.gov/pubmed/35181693
http://dx.doi.org/10.1038/s41598-022-06690-x
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author de Souza-Neto, Reinaldo Rodrigues
Carvalho, Isis Gabriela Barbosa
Martins, Paula Maria Moreira
Picchi, Simone Cristina
Tomaz, Juarez Pires
Caserta, Raquel
Takita, Marco Aurélio
de Souza, Alessandra Alves
author_facet de Souza-Neto, Reinaldo Rodrigues
Carvalho, Isis Gabriela Barbosa
Martins, Paula Maria Moreira
Picchi, Simone Cristina
Tomaz, Juarez Pires
Caserta, Raquel
Takita, Marco Aurélio
de Souza, Alessandra Alves
author_sort de Souza-Neto, Reinaldo Rodrigues
collection PubMed
description Type II toxin-antitoxin (TA) systems are widespread in bacteria and are involved in important cell features, such as cell growth inhibition and antimicrobial tolerance, through the induction of persister cells. Overall, these characteristics are associated with bacterial survival under stress conditions and represent a significant genetic mechanism to be explored for antibacterial molecules. We verified that even though Xylella fastidiosa and Xanthomonas citri subsp. citri share closely related genomes, they have different Type II TA system contents. One important difference is the absence of mqsRA in X. citri. The toxin component of this TA system has been shown to inhibit the growth of X. fastidiosa. Thus, the absence of mqsRA in X. citri led us to explore the possibility of using the MqsR toxin to impair X. citri growth. We purified MqsR and confirmed that the toxin was able to inhibit X. citri. Subsequently, transgenic citrus plants producing MqsR showed a significant reduction in citrus canker and citrus variegated chlorosis symptoms caused, respectively, by X. citri and X. fastidiosa. This study demonstrates that the use of toxins from TA systems is a promising strategy to be explored aiming bacterial control.
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spelling pubmed-88573202022-02-22 MqsR toxin as a biotechnological tool for plant pathogen bacterial control de Souza-Neto, Reinaldo Rodrigues Carvalho, Isis Gabriela Barbosa Martins, Paula Maria Moreira Picchi, Simone Cristina Tomaz, Juarez Pires Caserta, Raquel Takita, Marco Aurélio de Souza, Alessandra Alves Sci Rep Article Type II toxin-antitoxin (TA) systems are widespread in bacteria and are involved in important cell features, such as cell growth inhibition and antimicrobial tolerance, through the induction of persister cells. Overall, these characteristics are associated with bacterial survival under stress conditions and represent a significant genetic mechanism to be explored for antibacterial molecules. We verified that even though Xylella fastidiosa and Xanthomonas citri subsp. citri share closely related genomes, they have different Type II TA system contents. One important difference is the absence of mqsRA in X. citri. The toxin component of this TA system has been shown to inhibit the growth of X. fastidiosa. Thus, the absence of mqsRA in X. citri led us to explore the possibility of using the MqsR toxin to impair X. citri growth. We purified MqsR and confirmed that the toxin was able to inhibit X. citri. Subsequently, transgenic citrus plants producing MqsR showed a significant reduction in citrus canker and citrus variegated chlorosis symptoms caused, respectively, by X. citri and X. fastidiosa. This study demonstrates that the use of toxins from TA systems is a promising strategy to be explored aiming bacterial control. Nature Publishing Group UK 2022-02-18 /pmc/articles/PMC8857320/ /pubmed/35181693 http://dx.doi.org/10.1038/s41598-022-06690-x Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
de Souza-Neto, Reinaldo Rodrigues
Carvalho, Isis Gabriela Barbosa
Martins, Paula Maria Moreira
Picchi, Simone Cristina
Tomaz, Juarez Pires
Caserta, Raquel
Takita, Marco Aurélio
de Souza, Alessandra Alves
MqsR toxin as a biotechnological tool for plant pathogen bacterial control
title MqsR toxin as a biotechnological tool for plant pathogen bacterial control
title_full MqsR toxin as a biotechnological tool for plant pathogen bacterial control
title_fullStr MqsR toxin as a biotechnological tool for plant pathogen bacterial control
title_full_unstemmed MqsR toxin as a biotechnological tool for plant pathogen bacterial control
title_short MqsR toxin as a biotechnological tool for plant pathogen bacterial control
title_sort mqsr toxin as a biotechnological tool for plant pathogen bacterial control
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8857320/
https://www.ncbi.nlm.nih.gov/pubmed/35181693
http://dx.doi.org/10.1038/s41598-022-06690-x
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