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An adaptable live-cell imaging protocol to analyze organelle morphology in Saccharomyces cerevisiae
The protocol describes semiautomated live cell imaging in budding yeast. A key feature of the protocol is immobilizing cells in a culture dish, which allows for longer imaging times, changing culture media, or drug treatments. We describe steps for image acquisition and deconvolution, followed by ma...
| Autores principales: | , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Elsevier
2022
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8857511/ https://www.ncbi.nlm.nih.gov/pubmed/35243366 http://dx.doi.org/10.1016/j.xpro.2022.101124 |