LiverClear: A versatile protocol for mouse liver tissue clearing

Although there are numerous tissue clearing protocols, most are inadequate for clearing liver tissue. Here we present a flexible protocol for mouse liver tissue; we combine strategies from several previously published protocols for delipidation, decolorization, staining, and refractive index matchin...

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Detalles Bibliográficos
Autores principales: Molina, Laura M., Krutsenko, Yekaterina, Jenkins, Nathaniel E.C., Smith, Megan C., Tao, Junyan, Wheeler, Travis B., Watkins, Simon C., Watson, Alan M., Monga, Satdarshan P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8857608/
https://www.ncbi.nlm.nih.gov/pubmed/35243370
http://dx.doi.org/10.1016/j.xpro.2022.101178
Descripción
Sumario:Although there are numerous tissue clearing protocols, most are inadequate for clearing liver tissue. Here we present a flexible protocol for mouse liver tissue; we combine strategies from several previously published protocols for delipidation, decolorization, staining, and refractive index matching. LiverClear is sufficiently versatile to allow clearing of healthy and diseased mouse liver followed by immunofluorescence staining and imaging to visualize intact 3D structures such as bile ducts and hepatocyte canaliculi. We also adapted this protocol for clearing human livers. For complete details on the use and execution of this protocol, please refer to Molina et al. (2021).

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