A protocol for studying glucose homeostasis and islet function in mice

Glucose tolerance test and glucose stimulated insulin secretion are important measures to determine glucose homeostasis and islet function and assess diabetes. Here, we provide a protocol where glucose tolerance test is used to study glucose homeostasis and insulin secretion in vivo, followed by isl...

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Detalles Bibliográficos
Autores principales: Al Rijjal, Dana, Wheeler, Michael B.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8857654/
https://www.ncbi.nlm.nih.gov/pubmed/35243368
http://dx.doi.org/10.1016/j.xpro.2022.101171
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author Al Rijjal, Dana
Wheeler, Michael B.
author_facet Al Rijjal, Dana
Wheeler, Michael B.
author_sort Al Rijjal, Dana
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description Glucose tolerance test and glucose stimulated insulin secretion are important measures to determine glucose homeostasis and islet function and assess diabetes. Here, we provide a protocol where glucose tolerance test is used to study glucose homeostasis and insulin secretion in vivo, followed by islet isolation and glucose stimulated insulin secretion to determine islet function ex vivo. This protocol enables evaluation of glucose homeostasis and islets in mice which can also be applied to rat, beta cell lines and human studies. For complete details on the use and execution of this profile, please refer to Al Rijjal et al. (2021).
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spelling pubmed-88576542022-03-02 A protocol for studying glucose homeostasis and islet function in mice Al Rijjal, Dana Wheeler, Michael B. STAR Protoc Protocol Glucose tolerance test and glucose stimulated insulin secretion are important measures to determine glucose homeostasis and islet function and assess diabetes. Here, we provide a protocol where glucose tolerance test is used to study glucose homeostasis and insulin secretion in vivo, followed by islet isolation and glucose stimulated insulin secretion to determine islet function ex vivo. This protocol enables evaluation of glucose homeostasis and islets in mice which can also be applied to rat, beta cell lines and human studies. For complete details on the use and execution of this profile, please refer to Al Rijjal et al. (2021). Elsevier 2022-02-17 /pmc/articles/PMC8857654/ /pubmed/35243368 http://dx.doi.org/10.1016/j.xpro.2022.101171 Text en © 2022 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Al Rijjal, Dana
Wheeler, Michael B.
A protocol for studying glucose homeostasis and islet function in mice
title A protocol for studying glucose homeostasis and islet function in mice
title_full A protocol for studying glucose homeostasis and islet function in mice
title_fullStr A protocol for studying glucose homeostasis and islet function in mice
title_full_unstemmed A protocol for studying glucose homeostasis and islet function in mice
title_short A protocol for studying glucose homeostasis and islet function in mice
title_sort protocol for studying glucose homeostasis and islet function in mice
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8857654/
https://www.ncbi.nlm.nih.gov/pubmed/35243368
http://dx.doi.org/10.1016/j.xpro.2022.101171
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