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An integrated analysis of the competing endogenous RNA network associated of prognosis of stage I lung adenocarcinoma
BACKGROUND: Accumulating evidence indicates that long non-coding RNAs (lncRNAs) are involving in the tumorigenesis and metastasis of lung cancer. The aim of the study is to systematically characterize the lncRNA-associated competing endogenous RNA (ceRNA) network and identify key lncRNAs in the deve...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8857797/ https://www.ncbi.nlm.nih.gov/pubmed/35183135 http://dx.doi.org/10.1186/s12885-022-09290-0 |
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author | Xu, Yuan Lin, Guofu Liu, Yifei Lin, Xianbin Lin, Hai Guo, Zhifeng Xu, Yingxuan Lin, Qinhui Chen, Shaohua Yang, Jiansheng Zeng, Yiming |
author_facet | Xu, Yuan Lin, Guofu Liu, Yifei Lin, Xianbin Lin, Hai Guo, Zhifeng Xu, Yingxuan Lin, Qinhui Chen, Shaohua Yang, Jiansheng Zeng, Yiming |
author_sort | Xu, Yuan |
collection | PubMed |
description | BACKGROUND: Accumulating evidence indicates that long non-coding RNAs (lncRNAs) are involving in the tumorigenesis and metastasis of lung cancer. The aim of the study is to systematically characterize the lncRNA-associated competing endogenous RNA (ceRNA) network and identify key lncRNAs in the development of stage I lung adenocarcinoma (LUAD). METHODS: Totally, 1,955 DEmRNAs, 165 DEmiRNAs and 1,107 DElncRNAs were obtained in 10 paired normal and LUAD tissues. And a total of 8,912 paired lncRNA-miRNA-mRNA network was constructed. Using the Cancer Genome Atlas (TCGA) dataset, the module of ME turquoise was revealed to be most relevant to the progression of LUAD though Weighted Gene Co-expression Network Analysis (WGCNA). RESULTS: Of the lncRNAs identified, LINC00639, RP4-676L2.1 and FENDRR were in ceRNA network established by our RNA-sequencing dataset. Using univariate Cox regression analysis, FENDRR was a risk factor of progression free survival (PFS) of stage I LUAD patients (HRs = 1.69, 95%CI 1.07–2.68, P < .050). Subsequently, diffe rential expression of FENDRR in paired normal and LUAD tissues was detected significant by real-time quantitative (qRT-PCR) (P < 0.001). CONCLUSIONS: This study, for the first time, deciphered the regulatory role of FENDRR/miR-6815-5p axis in the progression of early-stage LUAD, which is needed to be established in vitro and in vivo. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12885-022-09290-0. |
format | Online Article Text |
id | pubmed-8857797 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-88577972022-02-23 An integrated analysis of the competing endogenous RNA network associated of prognosis of stage I lung adenocarcinoma Xu, Yuan Lin, Guofu Liu, Yifei Lin, Xianbin Lin, Hai Guo, Zhifeng Xu, Yingxuan Lin, Qinhui Chen, Shaohua Yang, Jiansheng Zeng, Yiming BMC Cancer Research BACKGROUND: Accumulating evidence indicates that long non-coding RNAs (lncRNAs) are involving in the tumorigenesis and metastasis of lung cancer. The aim of the study is to systematically characterize the lncRNA-associated competing endogenous RNA (ceRNA) network and identify key lncRNAs in the development of stage I lung adenocarcinoma (LUAD). METHODS: Totally, 1,955 DEmRNAs, 165 DEmiRNAs and 1,107 DElncRNAs were obtained in 10 paired normal and LUAD tissues. And a total of 8,912 paired lncRNA-miRNA-mRNA network was constructed. Using the Cancer Genome Atlas (TCGA) dataset, the module of ME turquoise was revealed to be most relevant to the progression of LUAD though Weighted Gene Co-expression Network Analysis (WGCNA). RESULTS: Of the lncRNAs identified, LINC00639, RP4-676L2.1 and FENDRR were in ceRNA network established by our RNA-sequencing dataset. Using univariate Cox regression analysis, FENDRR was a risk factor of progression free survival (PFS) of stage I LUAD patients (HRs = 1.69, 95%CI 1.07–2.68, P < .050). Subsequently, diffe rential expression of FENDRR in paired normal and LUAD tissues was detected significant by real-time quantitative (qRT-PCR) (P < 0.001). CONCLUSIONS: This study, for the first time, deciphered the regulatory role of FENDRR/miR-6815-5p axis in the progression of early-stage LUAD, which is needed to be established in vitro and in vivo. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12885-022-09290-0. BioMed Central 2022-02-19 /pmc/articles/PMC8857797/ /pubmed/35183135 http://dx.doi.org/10.1186/s12885-022-09290-0 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
spellingShingle | Research Xu, Yuan Lin, Guofu Liu, Yifei Lin, Xianbin Lin, Hai Guo, Zhifeng Xu, Yingxuan Lin, Qinhui Chen, Shaohua Yang, Jiansheng Zeng, Yiming An integrated analysis of the competing endogenous RNA network associated of prognosis of stage I lung adenocarcinoma |
title | An integrated analysis of the competing endogenous RNA network associated of prognosis of stage I lung adenocarcinoma |
title_full | An integrated analysis of the competing endogenous RNA network associated of prognosis of stage I lung adenocarcinoma |
title_fullStr | An integrated analysis of the competing endogenous RNA network associated of prognosis of stage I lung adenocarcinoma |
title_full_unstemmed | An integrated analysis of the competing endogenous RNA network associated of prognosis of stage I lung adenocarcinoma |
title_short | An integrated analysis of the competing endogenous RNA network associated of prognosis of stage I lung adenocarcinoma |
title_sort | integrated analysis of the competing endogenous rna network associated of prognosis of stage i lung adenocarcinoma |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8857797/ https://www.ncbi.nlm.nih.gov/pubmed/35183135 http://dx.doi.org/10.1186/s12885-022-09290-0 |
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