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Morphological characterization reveals new insights into giant cell development of Meloidogyne graminicola on rice

MAIN CONCLUSION: Three types of nematode-feeding sites (NFSs) caused by M. graminicola on rice were suggested, and the NFS polarized expansion stops before the full NFS maturation that occurs at adult female stage. ABSTRACT: Root-knot nematodes, Meloidogyne spp., secrete effectors and recruit host g...

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Autores principales: Niu, Yongrui, Xiao, Liying, de Almeida-Engler, Janice, Gheysen, Godelieve, Peng, Deliang, Xiao, Xueqiong, Huang, Wenkun, Wang, Gaofeng, Xiao, Yannong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8858295/
https://www.ncbi.nlm.nih.gov/pubmed/35184234
http://dx.doi.org/10.1007/s00425-022-03852-z
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author Niu, Yongrui
Xiao, Liying
de Almeida-Engler, Janice
Gheysen, Godelieve
Peng, Deliang
Xiao, Xueqiong
Huang, Wenkun
Wang, Gaofeng
Xiao, Yannong
author_facet Niu, Yongrui
Xiao, Liying
de Almeida-Engler, Janice
Gheysen, Godelieve
Peng, Deliang
Xiao, Xueqiong
Huang, Wenkun
Wang, Gaofeng
Xiao, Yannong
author_sort Niu, Yongrui
collection PubMed
description MAIN CONCLUSION: Three types of nematode-feeding sites (NFSs) caused by M. graminicola on rice were suggested, and the NFS polarized expansion stops before the full NFS maturation that occurs at adult female stage. ABSTRACT: Root-knot nematodes, Meloidogyne spp., secrete effectors and recruit host genes to establish their feeding sites giant cells, ensuring their nutrient acquisition. There is still a limited understanding of the mechanism underlying giant cell development. Here, the three-dimensional structures of M. graminicola-caused nematode-feeding sites (NFSs) on rice as well as changes in morphological features and cytoplasm density of the giant cells (GCs) during nematode parasitism were reconstructed and characterized by confocal microscopy and the Fiji software. Characterization of morphological features showed that three types of M. graminicola-caused NFSs, type I–III, were detected during parasitism at the second juvenile (J2), the third juvenile (J3), the fourth juvenile (J4) and adult female stages. Type I is the majority at all stages and type II develops into type I at J3 stage marked by its longitudinal growth. Meanwhile, NFSs underwent polarized expansion, where the lateral and longitudinal expansion ceased at later parasitic J2 stage and the non-feeding J4 stage, respectively. The investigation of giant cell cytoplasm density indicates that it reaches a peak at the midpoint of early parasitic J2 and adult female stages. Our data suggest the formation of three types of NFSs caused by M. graminicola on rice and the NFS polarized expansion stopping before full NFS maturation, which provides unprecedented spatio-temporal characterization of development of giant cells caused by a root-knot nematode. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s00425-022-03852-z.
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spelling pubmed-88582952022-02-23 Morphological characterization reveals new insights into giant cell development of Meloidogyne graminicola on rice Niu, Yongrui Xiao, Liying de Almeida-Engler, Janice Gheysen, Godelieve Peng, Deliang Xiao, Xueqiong Huang, Wenkun Wang, Gaofeng Xiao, Yannong Planta Original Article MAIN CONCLUSION: Three types of nematode-feeding sites (NFSs) caused by M. graminicola on rice were suggested, and the NFS polarized expansion stops before the full NFS maturation that occurs at adult female stage. ABSTRACT: Root-knot nematodes, Meloidogyne spp., secrete effectors and recruit host genes to establish their feeding sites giant cells, ensuring their nutrient acquisition. There is still a limited understanding of the mechanism underlying giant cell development. Here, the three-dimensional structures of M. graminicola-caused nematode-feeding sites (NFSs) on rice as well as changes in morphological features and cytoplasm density of the giant cells (GCs) during nematode parasitism were reconstructed and characterized by confocal microscopy and the Fiji software. Characterization of morphological features showed that three types of M. graminicola-caused NFSs, type I–III, were detected during parasitism at the second juvenile (J2), the third juvenile (J3), the fourth juvenile (J4) and adult female stages. Type I is the majority at all stages and type II develops into type I at J3 stage marked by its longitudinal growth. Meanwhile, NFSs underwent polarized expansion, where the lateral and longitudinal expansion ceased at later parasitic J2 stage and the non-feeding J4 stage, respectively. The investigation of giant cell cytoplasm density indicates that it reaches a peak at the midpoint of early parasitic J2 and adult female stages. Our data suggest the formation of three types of NFSs caused by M. graminicola on rice and the NFS polarized expansion stopping before full NFS maturation, which provides unprecedented spatio-temporal characterization of development of giant cells caused by a root-knot nematode. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s00425-022-03852-z. Springer Berlin Heidelberg 2022-02-19 2022 /pmc/articles/PMC8858295/ /pubmed/35184234 http://dx.doi.org/10.1007/s00425-022-03852-z Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Original Article
Niu, Yongrui
Xiao, Liying
de Almeida-Engler, Janice
Gheysen, Godelieve
Peng, Deliang
Xiao, Xueqiong
Huang, Wenkun
Wang, Gaofeng
Xiao, Yannong
Morphological characterization reveals new insights into giant cell development of Meloidogyne graminicola on rice
title Morphological characterization reveals new insights into giant cell development of Meloidogyne graminicola on rice
title_full Morphological characterization reveals new insights into giant cell development of Meloidogyne graminicola on rice
title_fullStr Morphological characterization reveals new insights into giant cell development of Meloidogyne graminicola on rice
title_full_unstemmed Morphological characterization reveals new insights into giant cell development of Meloidogyne graminicola on rice
title_short Morphological characterization reveals new insights into giant cell development of Meloidogyne graminicola on rice
title_sort morphological characterization reveals new insights into giant cell development of meloidogyne graminicola on rice
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8858295/
https://www.ncbi.nlm.nih.gov/pubmed/35184234
http://dx.doi.org/10.1007/s00425-022-03852-z
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