Analysis of unc-62 expression pattern in C. elegans embryonic AWC neurons

The Caenorhabditis elegans UNC-62 homothorax/Meis/TALE homeodomain protein functions sequentially to regulate general identity of the AWC olfactory neuron pair and the stochastic choice of asymmetric AWC subtypes during embryogenesis. Here we analyze the expression pattern of unc-62 during AWC devel...

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Detalles Bibliográficos
Autores principales: Hsieh, Yi-Wen, Chuang, Chiou-Fen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Caltech Library 2022
Materias:
Findings Previously Not Shown
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8858418/
https://www.ncbi.nlm.nih.gov/pubmed/35198862
http://dx.doi.org/10.17912/micropub.biology.000530
Descripción
Sumario:The Caenorhabditis elegans UNC-62 homothorax/Meis/TALE homeodomain protein functions sequentially to regulate general identity of the AWC olfactory neuron pair and the stochastic choice of asymmetric AWC subtypes during embryogenesis. Here we analyze the expression pattern of unc-62 during AWC development using an integrated unc-62::GFP fosmid rescuing transgene. UNC-62::GFP was not detected in AWC neurons in early or late embryos. These results are consistent with previous single-cell RNA sequencing data and also suggest an undetectable level of unc-62 expression and/or low stability of UNC-62 protein in AWC neurons during embryogenesis.

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