Analysis of unc-62 expression pattern in C. elegans embryonic AWC neurons

The Caenorhabditis elegans UNC-62 homothorax/Meis/TALE homeodomain protein functions sequentially to regulate general identity of the AWC olfactory neuron pair and the stochastic choice of asymmetric AWC subtypes during embryogenesis. Here we analyze the expression pattern of unc-62 during AWC devel...

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Detalles Bibliográficos
Autores principales: Hsieh, Yi-Wen, Chuang, Chiou-Fen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Caltech Library 2022
Materias:
Findings Previously Not Shown
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8858418/
https://www.ncbi.nlm.nih.gov/pubmed/35198862
http://dx.doi.org/10.17912/micropub.biology.000530
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author Hsieh, Yi-Wen
Chuang, Chiou-Fen
author_facet Hsieh, Yi-Wen
Chuang, Chiou-Fen
author_sort Hsieh, Yi-Wen
collection PubMed
description The Caenorhabditis elegans UNC-62 homothorax/Meis/TALE homeodomain protein functions sequentially to regulate general identity of the AWC olfactory neuron pair and the stochastic choice of asymmetric AWC subtypes during embryogenesis. Here we analyze the expression pattern of unc-62 during AWC development using an integrated unc-62::GFP fosmid rescuing transgene. UNC-62::GFP was not detected in AWC neurons in early or late embryos. These results are consistent with previous single-cell RNA sequencing data and also suggest an undetectable level of unc-62 expression and/or low stability of UNC-62 protein in AWC neurons during embryogenesis.
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spelling pubmed-88584182022-02-22 Analysis of unc-62 expression pattern in C. elegans embryonic AWC neurons Hsieh, Yi-Wen Chuang, Chiou-Fen MicroPubl Biol Findings Previously Not Shown The Caenorhabditis elegans UNC-62 homothorax/Meis/TALE homeodomain protein functions sequentially to regulate general identity of the AWC olfactory neuron pair and the stochastic choice of asymmetric AWC subtypes during embryogenesis. Here we analyze the expression pattern of unc-62 during AWC development using an integrated unc-62::GFP fosmid rescuing transgene. UNC-62::GFP was not detected in AWC neurons in early or late embryos. These results are consistent with previous single-cell RNA sequencing data and also suggest an undetectable level of unc-62 expression and/or low stability of UNC-62 protein in AWC neurons during embryogenesis. Caltech Library 2022-02-18 /pmc/articles/PMC8858418/ /pubmed/35198862 http://dx.doi.org/10.17912/micropub.biology.000530 Text en Copyright: © 2022 by the authors https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Findings Previously Not Shown
Hsieh, Yi-Wen
Chuang, Chiou-Fen
Analysis of unc-62 expression pattern in C. elegans embryonic AWC neurons
title Analysis of unc-62 expression pattern in C. elegans embryonic AWC neurons
title_full Analysis of unc-62 expression pattern in C. elegans embryonic AWC neurons
title_fullStr Analysis of unc-62 expression pattern in C. elegans embryonic AWC neurons
title_full_unstemmed Analysis of unc-62 expression pattern in C. elegans embryonic AWC neurons
title_short Analysis of unc-62 expression pattern in C. elegans embryonic AWC neurons
title_sort analysis of unc-62 expression pattern in c. elegans embryonic awc neurons
topic Findings Previously Not Shown
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8858418/
https://www.ncbi.nlm.nih.gov/pubmed/35198862
http://dx.doi.org/10.17912/micropub.biology.000530
work_keys_str_mv AT hsiehyiwen analysisofunc62expressionpatternincelegansembryonicawcneurons
AT chuangchioufen analysisofunc62expressionpatternincelegansembryonicawcneurons

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