Cargando…

Bacterial Genes Encoding Resistance Against Antibiotics and Metals in Well-Maintained Drinking Water Distribution Systems in Finland

Information on the co-occurrence of antibiotic resistance genes (ARGs) and metal resistance genes (MRGs) among bacterial communities in drinking water distribution systems (DWDSs) is scarce. This study characterized ARGs and MRGs in five well-maintained DWDSs in Finland. The studied DWDSs had differ...

Descripción completa

Detalles Bibliográficos
Autores principales: Tiwari, Ananda, Gomez-Alvarez, Vicente, Siponen, Sallamaari, Sarekoski, Anniina, Hokajärvi, Anna-Maria, Kauppinen, Ari, Torvinen, Eila, Miettinen, Ilkka T., Pitkänen, Tarja
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8859300/
https://www.ncbi.nlm.nih.gov/pubmed/35197945
http://dx.doi.org/10.3389/fmicb.2021.803094
_version_ 1784654426739310592
author Tiwari, Ananda
Gomez-Alvarez, Vicente
Siponen, Sallamaari
Sarekoski, Anniina
Hokajärvi, Anna-Maria
Kauppinen, Ari
Torvinen, Eila
Miettinen, Ilkka T.
Pitkänen, Tarja
author_facet Tiwari, Ananda
Gomez-Alvarez, Vicente
Siponen, Sallamaari
Sarekoski, Anniina
Hokajärvi, Anna-Maria
Kauppinen, Ari
Torvinen, Eila
Miettinen, Ilkka T.
Pitkänen, Tarja
author_sort Tiwari, Ananda
collection PubMed
description Information on the co-occurrence of antibiotic resistance genes (ARGs) and metal resistance genes (MRGs) among bacterial communities in drinking water distribution systems (DWDSs) is scarce. This study characterized ARGs and MRGs in five well-maintained DWDSs in Finland. The studied DWDSs had different raw water sources and treatment methods. Two of the waterworks employed artificially recharged groundwater (ARGW) and used no disinfection in the treatment process. The other three waterworks (two surface and one groundwater source) used UV light and chlorine during the treatment process. Ten bulk water samples (two from each DWDS) were collected, and environmental DNA was extracted and then sequenced using the Illumina HiSeq platform for high-throughput shotgun metagenome sequencing. A total of 430 ARGs were characterized among all samples with the highest diversity of ARGs identified from samples collected from non-disinfected DWDSs. Furthermore, non-disinfected DWDSs contained the highest diversity of bacterial communities. However, samples from DWDSs using disinfectants contained over double the ratio of ARG reads to 16S rRNA gene reads and most of the MRG (namely mercury and arsenic resistance genes). The total reads and types of ARGs conferring genes associated with antibiotic groups namely multidrug resistance, and bacitracin, beta-lactam, and aminoglycoside and mercury resistance genes increased in waterworks treating surface water with disinfection. The findings of this study contribute toward a comprehensive understanding of ARGs and MRGs in DWDSs. The occurrence of bacteria carrying antibiotic or metal resistance genes in drinking water causes direct exposure to people, and thus, more systematic investigation is needed to decipher the potential effect of these resistomes on human health.
format Online
Article
Text
id pubmed-8859300
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-88593002022-02-22 Bacterial Genes Encoding Resistance Against Antibiotics and Metals in Well-Maintained Drinking Water Distribution Systems in Finland Tiwari, Ananda Gomez-Alvarez, Vicente Siponen, Sallamaari Sarekoski, Anniina Hokajärvi, Anna-Maria Kauppinen, Ari Torvinen, Eila Miettinen, Ilkka T. Pitkänen, Tarja Front Microbiol Microbiology Information on the co-occurrence of antibiotic resistance genes (ARGs) and metal resistance genes (MRGs) among bacterial communities in drinking water distribution systems (DWDSs) is scarce. This study characterized ARGs and MRGs in five well-maintained DWDSs in Finland. The studied DWDSs had different raw water sources and treatment methods. Two of the waterworks employed artificially recharged groundwater (ARGW) and used no disinfection in the treatment process. The other three waterworks (two surface and one groundwater source) used UV light and chlorine during the treatment process. Ten bulk water samples (two from each DWDS) were collected, and environmental DNA was extracted and then sequenced using the Illumina HiSeq platform for high-throughput shotgun metagenome sequencing. A total of 430 ARGs were characterized among all samples with the highest diversity of ARGs identified from samples collected from non-disinfected DWDSs. Furthermore, non-disinfected DWDSs contained the highest diversity of bacterial communities. However, samples from DWDSs using disinfectants contained over double the ratio of ARG reads to 16S rRNA gene reads and most of the MRG (namely mercury and arsenic resistance genes). The total reads and types of ARGs conferring genes associated with antibiotic groups namely multidrug resistance, and bacitracin, beta-lactam, and aminoglycoside and mercury resistance genes increased in waterworks treating surface water with disinfection. The findings of this study contribute toward a comprehensive understanding of ARGs and MRGs in DWDSs. The occurrence of bacteria carrying antibiotic or metal resistance genes in drinking water causes direct exposure to people, and thus, more systematic investigation is needed to decipher the potential effect of these resistomes on human health. Frontiers Media S.A. 2022-02-07 /pmc/articles/PMC8859300/ /pubmed/35197945 http://dx.doi.org/10.3389/fmicb.2021.803094 Text en Copyright © 2022 Tiwari, Gomez-Alvarez, Siponen, Sarekoski, Hokajärvi, Kauppinen, Torvinen, Miettinen and Pitkänen. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Tiwari, Ananda
Gomez-Alvarez, Vicente
Siponen, Sallamaari
Sarekoski, Anniina
Hokajärvi, Anna-Maria
Kauppinen, Ari
Torvinen, Eila
Miettinen, Ilkka T.
Pitkänen, Tarja
Bacterial Genes Encoding Resistance Against Antibiotics and Metals in Well-Maintained Drinking Water Distribution Systems in Finland
title Bacterial Genes Encoding Resistance Against Antibiotics and Metals in Well-Maintained Drinking Water Distribution Systems in Finland
title_full Bacterial Genes Encoding Resistance Against Antibiotics and Metals in Well-Maintained Drinking Water Distribution Systems in Finland
title_fullStr Bacterial Genes Encoding Resistance Against Antibiotics and Metals in Well-Maintained Drinking Water Distribution Systems in Finland
title_full_unstemmed Bacterial Genes Encoding Resistance Against Antibiotics and Metals in Well-Maintained Drinking Water Distribution Systems in Finland
title_short Bacterial Genes Encoding Resistance Against Antibiotics and Metals in Well-Maintained Drinking Water Distribution Systems in Finland
title_sort bacterial genes encoding resistance against antibiotics and metals in well-maintained drinking water distribution systems in finland
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8859300/
https://www.ncbi.nlm.nih.gov/pubmed/35197945
http://dx.doi.org/10.3389/fmicb.2021.803094
work_keys_str_mv AT tiwariananda bacterialgenesencodingresistanceagainstantibioticsandmetalsinwellmaintaineddrinkingwaterdistributionsystemsinfinland
AT gomezalvarezvicente bacterialgenesencodingresistanceagainstantibioticsandmetalsinwellmaintaineddrinkingwaterdistributionsystemsinfinland
AT siponensallamaari bacterialgenesencodingresistanceagainstantibioticsandmetalsinwellmaintaineddrinkingwaterdistributionsystemsinfinland
AT sarekoskianniina bacterialgenesencodingresistanceagainstantibioticsandmetalsinwellmaintaineddrinkingwaterdistributionsystemsinfinland
AT hokajarviannamaria bacterialgenesencodingresistanceagainstantibioticsandmetalsinwellmaintaineddrinkingwaterdistributionsystemsinfinland
AT kauppinenari bacterialgenesencodingresistanceagainstantibioticsandmetalsinwellmaintaineddrinkingwaterdistributionsystemsinfinland
AT torvineneila bacterialgenesencodingresistanceagainstantibioticsandmetalsinwellmaintaineddrinkingwaterdistributionsystemsinfinland
AT miettinenilkkat bacterialgenesencodingresistanceagainstantibioticsandmetalsinwellmaintaineddrinkingwaterdistributionsystemsinfinland
AT pitkanentarja bacterialgenesencodingresistanceagainstantibioticsandmetalsinwellmaintaineddrinkingwaterdistributionsystemsinfinland