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RNA-Seq Technology Reveals the Mechanism of SDT Combined With Novel Nanobubbles Against HCC
Sonodynamic therapy is widely used in the treatment and research of hepatocellular carcinoma. A novel targeted nanobubble complex mediated with Hematoporphyrin monomethyl ether and Lonidamine was structured as a sensitizer, characterized the properties, and studied the therapeutic effect on hepatoce...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8859324/ https://www.ncbi.nlm.nih.gov/pubmed/35198599 http://dx.doi.org/10.3389/fmolb.2021.791331 |
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author | Shang, Haitao Chen, Yichi Wang, Chunyue Zhang, Shentao Wu, Bolin Liang, Xitian Liu, Zhao Wang, Qiucheng Cheng, Wen |
author_facet | Shang, Haitao Chen, Yichi Wang, Chunyue Zhang, Shentao Wu, Bolin Liang, Xitian Liu, Zhao Wang, Qiucheng Cheng, Wen |
author_sort | Shang, Haitao |
collection | PubMed |
description | Sonodynamic therapy is widely used in the treatment and research of hepatocellular carcinoma. A novel targeted nanobubble complex mediated with Hematoporphyrin monomethyl ether and Lonidamine was structured as a sensitizer, characterized the properties, and studied the therapeutic effect on hepatocellular carcinoma. The complexes can promote the apoptosis of hepatocellular carcinoma cells and work better in combination with sonodynamic therapy. The differential expression of multiple types of RNA in hepatocellular carcinoma with sonodynamic therapy can be identified accurately with high-throughput RNA sequencing. The differential expressions of mRNA, lncRNA, and circRNA were analyzed by RNA-Seq. The enrichment analyses (Gene Ontology and KEGG) prompted the meaningful genes and pathways in the process of sonodynamic therapy in hepatocellular carcinoma cells. HMME-LND@C(3)F(8)-NBs conjugated with ultrasound is confirmed efficiently for inhibiting the development of hepatocellular carcinoma cells, and it is a combination of multiple genes and mechanisms. |
format | Online Article Text |
id | pubmed-8859324 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-88593242022-02-22 RNA-Seq Technology Reveals the Mechanism of SDT Combined With Novel Nanobubbles Against HCC Shang, Haitao Chen, Yichi Wang, Chunyue Zhang, Shentao Wu, Bolin Liang, Xitian Liu, Zhao Wang, Qiucheng Cheng, Wen Front Mol Biosci Molecular Biosciences Sonodynamic therapy is widely used in the treatment and research of hepatocellular carcinoma. A novel targeted nanobubble complex mediated with Hematoporphyrin monomethyl ether and Lonidamine was structured as a sensitizer, characterized the properties, and studied the therapeutic effect on hepatocellular carcinoma. The complexes can promote the apoptosis of hepatocellular carcinoma cells and work better in combination with sonodynamic therapy. The differential expression of multiple types of RNA in hepatocellular carcinoma with sonodynamic therapy can be identified accurately with high-throughput RNA sequencing. The differential expressions of mRNA, lncRNA, and circRNA were analyzed by RNA-Seq. The enrichment analyses (Gene Ontology and KEGG) prompted the meaningful genes and pathways in the process of sonodynamic therapy in hepatocellular carcinoma cells. HMME-LND@C(3)F(8)-NBs conjugated with ultrasound is confirmed efficiently for inhibiting the development of hepatocellular carcinoma cells, and it is a combination of multiple genes and mechanisms. Frontiers Media S.A. 2022-02-07 /pmc/articles/PMC8859324/ /pubmed/35198599 http://dx.doi.org/10.3389/fmolb.2021.791331 Text en Copyright © 2022 Shang, Chen, Wang, Zhang, Wu, Liang, Liu, Wang and Cheng. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Molecular Biosciences Shang, Haitao Chen, Yichi Wang, Chunyue Zhang, Shentao Wu, Bolin Liang, Xitian Liu, Zhao Wang, Qiucheng Cheng, Wen RNA-Seq Technology Reveals the Mechanism of SDT Combined With Novel Nanobubbles Against HCC |
title | RNA-Seq Technology Reveals the Mechanism of SDT Combined With Novel Nanobubbles Against HCC |
title_full | RNA-Seq Technology Reveals the Mechanism of SDT Combined With Novel Nanobubbles Against HCC |
title_fullStr | RNA-Seq Technology Reveals the Mechanism of SDT Combined With Novel Nanobubbles Against HCC |
title_full_unstemmed | RNA-Seq Technology Reveals the Mechanism of SDT Combined With Novel Nanobubbles Against HCC |
title_short | RNA-Seq Technology Reveals the Mechanism of SDT Combined With Novel Nanobubbles Against HCC |
title_sort | rna-seq technology reveals the mechanism of sdt combined with novel nanobubbles against hcc |
topic | Molecular Biosciences |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8859324/ https://www.ncbi.nlm.nih.gov/pubmed/35198599 http://dx.doi.org/10.3389/fmolb.2021.791331 |
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