Cargando…

Demographic study of 366 cases of oral leukoplakia and immunohistochemical analysis – An institutional study

BACKGROUND: It has been reported that oral squamous cell carcinoma (OSCC) is associated with the presence of potentially malignant disorders (PMDs) in 15%–48% of cases. Among PMDs, oral leukoplakia (OL) is the most common, with 16%–62% of cases associated with OSCC. Hence, in the present study, we h...

Descripción completa

Detalles Bibliográficos
Autores principales: Ahire, Manisha Sardar, D′Souza, Zaneta Ivy, Chettiankandy, Tabita Joy, Nagar, Saurabh R., Sinha, Anuradha, Tupkari, Jagdish V.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Medknow Publications & Media Pvt Ltd 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8859585/
https://www.ncbi.nlm.nih.gov/pubmed/35281146
http://dx.doi.org/10.4103/jomfp.jomfp_228_21
_version_ 1784654494469980160
author Ahire, Manisha Sardar
D′Souza, Zaneta Ivy
Chettiankandy, Tabita Joy
Nagar, Saurabh R.
Sinha, Anuradha
Tupkari, Jagdish V.
author_facet Ahire, Manisha Sardar
D′Souza, Zaneta Ivy
Chettiankandy, Tabita Joy
Nagar, Saurabh R.
Sinha, Anuradha
Tupkari, Jagdish V.
author_sort Ahire, Manisha Sardar
collection PubMed
description BACKGROUND: It has been reported that oral squamous cell carcinoma (OSCC) is associated with the presence of potentially malignant disorders (PMDs) in 15%–48% of cases. Among PMDs, oral leukoplakia (OL) is the most common, with 16%–62% of cases associated with OSCC. Hence, in the present study, we have analyzed demographic data and re-evaluated immunohistochemical (IHC) data of OL cases and aimed to correlate the clinical, histopathological and IHC aspects of OL. MATERIALS AND METHODS: The data of histopathologically diagnosed cases of OL were retrieved from the archives. These data were further evaluated for age, gender, duration, site, size, side, habits, clinical staging and histopathological grading. IHC re-evaluation of OL tissues was done using epithelial cadherin (E-cadherin), n = 20; human MutL homolog 1 (hMLH1), n = 30; CD1a (n = 30); vimentin (n = 30); Ki-67 (n = 30); heat shock protein-70 (HSP-70), n = 30; p16(INK4), n = 20; and mucin-1 (MUC1), n = 30. All the results and observations were subjected to descriptive statistical analysis. RESULTS: The male: female ratio was 7.5:1; right side and buccal mucosa were more commonly affected. The duration of the lesion ranged from 1 to 30 years. One hundred and twelve patients were habituated to tobacco chewing, while 171 patients came with a combined habit of smoke and smokeless tobacco usage. Clinically, most of the lesions were of stage 2 while histopathologically they were of mild dysplasia. There was a decrease in the immunoexpression of E-cadherin, hMLH1 and CD1a, while there was an increase in the immunoexpression of vimentin, Ki-67, HSP-70, MUC1 and p16(INK4). CONCLUSION: The study of different biomarkers such as cytoplasmic, membranous and nuclear in OL will help in better understanding and application of a reliable marker for diagnostic and prognostic purpose.
format Online
Article
Text
id pubmed-8859585
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher Medknow Publications & Media Pvt Ltd
record_format MEDLINE/PubMed
spelling pubmed-88595852022-03-10 Demographic study of 366 cases of oral leukoplakia and immunohistochemical analysis – An institutional study Ahire, Manisha Sardar D′Souza, Zaneta Ivy Chettiankandy, Tabita Joy Nagar, Saurabh R. Sinha, Anuradha Tupkari, Jagdish V. J Oral Maxillofac Pathol Original Article BACKGROUND: It has been reported that oral squamous cell carcinoma (OSCC) is associated with the presence of potentially malignant disorders (PMDs) in 15%–48% of cases. Among PMDs, oral leukoplakia (OL) is the most common, with 16%–62% of cases associated with OSCC. Hence, in the present study, we have analyzed demographic data and re-evaluated immunohistochemical (IHC) data of OL cases and aimed to correlate the clinical, histopathological and IHC aspects of OL. MATERIALS AND METHODS: The data of histopathologically diagnosed cases of OL were retrieved from the archives. These data were further evaluated for age, gender, duration, site, size, side, habits, clinical staging and histopathological grading. IHC re-evaluation of OL tissues was done using epithelial cadherin (E-cadherin), n = 20; human MutL homolog 1 (hMLH1), n = 30; CD1a (n = 30); vimentin (n = 30); Ki-67 (n = 30); heat shock protein-70 (HSP-70), n = 30; p16(INK4), n = 20; and mucin-1 (MUC1), n = 30. All the results and observations were subjected to descriptive statistical analysis. RESULTS: The male: female ratio was 7.5:1; right side and buccal mucosa were more commonly affected. The duration of the lesion ranged from 1 to 30 years. One hundred and twelve patients were habituated to tobacco chewing, while 171 patients came with a combined habit of smoke and smokeless tobacco usage. Clinically, most of the lesions were of stage 2 while histopathologically they were of mild dysplasia. There was a decrease in the immunoexpression of E-cadherin, hMLH1 and CD1a, while there was an increase in the immunoexpression of vimentin, Ki-67, HSP-70, MUC1 and p16(INK4). CONCLUSION: The study of different biomarkers such as cytoplasmic, membranous and nuclear in OL will help in better understanding and application of a reliable marker for diagnostic and prognostic purpose. Medknow Publications & Media Pvt Ltd 2021 2022-01-11 /pmc/articles/PMC8859585/ /pubmed/35281146 http://dx.doi.org/10.4103/jomfp.jomfp_228_21 Text en Copyright: © 2022 Journal of Oral and Maxillofacial Pathology https://creativecommons.org/licenses/by-nc-sa/4.0/This is an open access journal, and articles are distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as appropriate credit is given and the new creations are licensed under the identical terms.
spellingShingle Original Article
Ahire, Manisha Sardar
D′Souza, Zaneta Ivy
Chettiankandy, Tabita Joy
Nagar, Saurabh R.
Sinha, Anuradha
Tupkari, Jagdish V.
Demographic study of 366 cases of oral leukoplakia and immunohistochemical analysis – An institutional study
title Demographic study of 366 cases of oral leukoplakia and immunohistochemical analysis – An institutional study
title_full Demographic study of 366 cases of oral leukoplakia and immunohistochemical analysis – An institutional study
title_fullStr Demographic study of 366 cases of oral leukoplakia and immunohistochemical analysis – An institutional study
title_full_unstemmed Demographic study of 366 cases of oral leukoplakia and immunohistochemical analysis – An institutional study
title_short Demographic study of 366 cases of oral leukoplakia and immunohistochemical analysis – An institutional study
title_sort demographic study of 366 cases of oral leukoplakia and immunohistochemical analysis – an institutional study
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8859585/
https://www.ncbi.nlm.nih.gov/pubmed/35281146
http://dx.doi.org/10.4103/jomfp.jomfp_228_21
work_keys_str_mv AT ahiremanishasardar demographicstudyof366casesoforalleukoplakiaandimmunohistochemicalanalysisaninstitutionalstudy
AT dsouzazanetaivy demographicstudyof366casesoforalleukoplakiaandimmunohistochemicalanalysisaninstitutionalstudy
AT chettiankandytabitajoy demographicstudyof366casesoforalleukoplakiaandimmunohistochemicalanalysisaninstitutionalstudy
AT nagarsaurabhr demographicstudyof366casesoforalleukoplakiaandimmunohistochemicalanalysisaninstitutionalstudy
AT sinhaanuradha demographicstudyof366casesoforalleukoplakiaandimmunohistochemicalanalysisaninstitutionalstudy
AT tupkarijagdishv demographicstudyof366casesoforalleukoplakiaandimmunohistochemicalanalysisaninstitutionalstudy