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Assessment of podoplanin lymphatic vessel density in oral epithelial dysplasia

BACKGROUND: Oral squamous cell carcinoma (OSCC) can be preceded by the appearance of lesions which have the potential to develop into cancer. Recently, it was suggested that the tumor-associated lymphatic vessels formation plays an active role in tumor progression and metastasis of several human mal...

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Autores principales: Lunawat, Snehal Dilip, Prakash, Nilima, Pradeep, G. L., Chaware, Smita Jembu, Chaudhari, Nayana Rajendra, Salunkhe, Vaibhavi Parikshit
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Medknow Publications & Media Pvt Ltd 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8859614/
https://www.ncbi.nlm.nih.gov/pubmed/35281144
http://dx.doi.org/10.4103/jomfp.JOMFP_103_20
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author Lunawat, Snehal Dilip
Prakash, Nilima
Pradeep, G. L.
Chaware, Smita Jembu
Chaudhari, Nayana Rajendra
Salunkhe, Vaibhavi Parikshit
author_facet Lunawat, Snehal Dilip
Prakash, Nilima
Pradeep, G. L.
Chaware, Smita Jembu
Chaudhari, Nayana Rajendra
Salunkhe, Vaibhavi Parikshit
author_sort Lunawat, Snehal Dilip
collection PubMed
description BACKGROUND: Oral squamous cell carcinoma (OSCC) can be preceded by the appearance of lesions which have the potential to develop into cancer. Recently, it was suggested that the tumor-associated lymphatic vessels formation plays an active role in tumor progression and metastasis of several human malignancies including OSCC. There is the view that, in an individual lesion, the more severe the dysplasia, the greater the likelihood is of progression to malignancy. AIM: This study is aimed to investigate podoplanin (PDPN) immunoexpression in lymphatic vessels of oral epithelial dysplasia (OED) and to assess the lymphatic vessel density (LVD) in histologic grades of OED. MATERIALS AND METHODS: The study group comprised thirty histopathologically diagnosed cases of OED with various grades of differentiation and thirty cases of clinically normal oral mucosa. After immunohistochemical staining, cases of OED were immunohistochemically analyzed quantitatively for PDPN (D2-40) LVD. STATISTICAL ANALYSIS: The statistical analysis was done using Kruskal Wallis analysis of variance; pair-wise Tukey's post hoc test was applied to evaluate the significant differences among the mean values in different groups. Results with “P < 0.05” were considered to be statistically significant at 95% of confidence level. RESULTS: PDPN LVD scores increased with increasing grades of dysplasia. Pair-wise comparisons of the PDPN LVD scores and the histopathologic grades of OED were found to be statistically significant (P < 0.05). CONCLUSIONS: Increase in PDPN LVD in OED represents a promising tool for more wide spread studies of tumor lymphangiogenesis and its role in progression of dysplastic lesion to human cancer.
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spelling pubmed-88596142022-03-10 Assessment of podoplanin lymphatic vessel density in oral epithelial dysplasia Lunawat, Snehal Dilip Prakash, Nilima Pradeep, G. L. Chaware, Smita Jembu Chaudhari, Nayana Rajendra Salunkhe, Vaibhavi Parikshit J Oral Maxillofac Pathol Original Article BACKGROUND: Oral squamous cell carcinoma (OSCC) can be preceded by the appearance of lesions which have the potential to develop into cancer. Recently, it was suggested that the tumor-associated lymphatic vessels formation plays an active role in tumor progression and metastasis of several human malignancies including OSCC. There is the view that, in an individual lesion, the more severe the dysplasia, the greater the likelihood is of progression to malignancy. AIM: This study is aimed to investigate podoplanin (PDPN) immunoexpression in lymphatic vessels of oral epithelial dysplasia (OED) and to assess the lymphatic vessel density (LVD) in histologic grades of OED. MATERIALS AND METHODS: The study group comprised thirty histopathologically diagnosed cases of OED with various grades of differentiation and thirty cases of clinically normal oral mucosa. After immunohistochemical staining, cases of OED were immunohistochemically analyzed quantitatively for PDPN (D2-40) LVD. STATISTICAL ANALYSIS: The statistical analysis was done using Kruskal Wallis analysis of variance; pair-wise Tukey's post hoc test was applied to evaluate the significant differences among the mean values in different groups. Results with “P < 0.05” were considered to be statistically significant at 95% of confidence level. RESULTS: PDPN LVD scores increased with increasing grades of dysplasia. Pair-wise comparisons of the PDPN LVD scores and the histopathologic grades of OED were found to be statistically significant (P < 0.05). CONCLUSIONS: Increase in PDPN LVD in OED represents a promising tool for more wide spread studies of tumor lymphangiogenesis and its role in progression of dysplastic lesion to human cancer. Medknow Publications & Media Pvt Ltd 2021 2022-01-11 /pmc/articles/PMC8859614/ /pubmed/35281144 http://dx.doi.org/10.4103/jomfp.JOMFP_103_20 Text en Copyright: © 2022 Journal of Oral and Maxillofacial Pathology https://creativecommons.org/licenses/by-nc-sa/4.0/This is an open access journal, and articles are distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as appropriate credit is given and the new creations are licensed under the identical terms.
spellingShingle Original Article
Lunawat, Snehal Dilip
Prakash, Nilima
Pradeep, G. L.
Chaware, Smita Jembu
Chaudhari, Nayana Rajendra
Salunkhe, Vaibhavi Parikshit
Assessment of podoplanin lymphatic vessel density in oral epithelial dysplasia
title Assessment of podoplanin lymphatic vessel density in oral epithelial dysplasia
title_full Assessment of podoplanin lymphatic vessel density in oral epithelial dysplasia
title_fullStr Assessment of podoplanin lymphatic vessel density in oral epithelial dysplasia
title_full_unstemmed Assessment of podoplanin lymphatic vessel density in oral epithelial dysplasia
title_short Assessment of podoplanin lymphatic vessel density in oral epithelial dysplasia
title_sort assessment of podoplanin lymphatic vessel density in oral epithelial dysplasia
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8859614/
https://www.ncbi.nlm.nih.gov/pubmed/35281144
http://dx.doi.org/10.4103/jomfp.JOMFP_103_20
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