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Immunohistochemical evaluation of myofibroblasts in oral epithelial dysplasia and oral squamous cell carcinoma
AIM: The aim of the study is to evaluate the presence of myofibroblasts quantitatively in oral epithelial dysplasia, oral squamous cell carcinoma (OSCC). MATERIALS AND METHODS: Formalin-fixed, paraffin-embedded blocks were retrieved from the institutional archives. The sample size is 35 and included...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Medknow Publications & Media Pvt Ltd
2021
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8859618/ https://www.ncbi.nlm.nih.gov/pubmed/35281169 http://dx.doi.org/10.4103/jomfp.jomfp_505_20 |
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author | Pinisetti, Soujanya Tadi, Durgaprasad Manyam, Ravikanth Alla, Ramya |
author_facet | Pinisetti, Soujanya Tadi, Durgaprasad Manyam, Ravikanth Alla, Ramya |
author_sort | Pinisetti, Soujanya |
collection | PubMed |
description | AIM: The aim of the study is to evaluate the presence of myofibroblasts quantitatively in oral epithelial dysplasia, oral squamous cell carcinoma (OSCC). MATERIALS AND METHODS: Formalin-fixed, paraffin-embedded blocks were retrieved from the institutional archives. The sample size is 35 and included 15 cases of oral epithelial dysplasia (n = 15), 15 cases of squamous cell carcinoma (n = 15) and 5 cases of normal oral mucosa which served as the control (n = 5). Histologic sections were subjected to immunohistochemical analysis using alpha-smooth muscle actin, and the mean number of myofibroblasts was evaluated. RESULTS: There were no myofibroblasts in the stroma of normal oral mucosa and oral epithelial dysplasia. Whereas all cases of OSCC showed myofibroblasts (mean ± standard deviation: 21.49 ± 9.76). This difference of myofibroblasts between OSCC and oral epithelial dysplasia was statistically significant with a P < 0.05. There was no statistically significant difference in the mean number of Myofibroblasts(MF) between 3 histologic grades of OSCC. CONCLUSION: The presence of myofibroblasts in the stroma of OSCC and their absence in normal oral mucosa and epithelial dysplasia reveals that these cells may play a role in cancer cell invasion and progression so the treatment strategies targeting the myofibroblasts and their by products may be beneficial in OSCC patients. |
format | Online Article Text |
id | pubmed-8859618 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Medknow Publications & Media Pvt Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-88596182022-03-10 Immunohistochemical evaluation of myofibroblasts in oral epithelial dysplasia and oral squamous cell carcinoma Pinisetti, Soujanya Tadi, Durgaprasad Manyam, Ravikanth Alla, Ramya J Oral Maxillofac Pathol Original Article AIM: The aim of the study is to evaluate the presence of myofibroblasts quantitatively in oral epithelial dysplasia, oral squamous cell carcinoma (OSCC). MATERIALS AND METHODS: Formalin-fixed, paraffin-embedded blocks were retrieved from the institutional archives. The sample size is 35 and included 15 cases of oral epithelial dysplasia (n = 15), 15 cases of squamous cell carcinoma (n = 15) and 5 cases of normal oral mucosa which served as the control (n = 5). Histologic sections were subjected to immunohistochemical analysis using alpha-smooth muscle actin, and the mean number of myofibroblasts was evaluated. RESULTS: There were no myofibroblasts in the stroma of normal oral mucosa and oral epithelial dysplasia. Whereas all cases of OSCC showed myofibroblasts (mean ± standard deviation: 21.49 ± 9.76). This difference of myofibroblasts between OSCC and oral epithelial dysplasia was statistically significant with a P < 0.05. There was no statistically significant difference in the mean number of Myofibroblasts(MF) between 3 histologic grades of OSCC. CONCLUSION: The presence of myofibroblasts in the stroma of OSCC and their absence in normal oral mucosa and epithelial dysplasia reveals that these cells may play a role in cancer cell invasion and progression so the treatment strategies targeting the myofibroblasts and their by products may be beneficial in OSCC patients. Medknow Publications & Media Pvt Ltd 2021 2022-01-11 /pmc/articles/PMC8859618/ /pubmed/35281169 http://dx.doi.org/10.4103/jomfp.jomfp_505_20 Text en Copyright: © 2022 Journal of Oral and Maxillofacial Pathology https://creativecommons.org/licenses/by-nc-sa/4.0/This is an open access journal, and articles are distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as appropriate credit is given and the new creations are licensed under the identical terms. |
spellingShingle | Original Article Pinisetti, Soujanya Tadi, Durgaprasad Manyam, Ravikanth Alla, Ramya Immunohistochemical evaluation of myofibroblasts in oral epithelial dysplasia and oral squamous cell carcinoma |
title | Immunohistochemical evaluation of myofibroblasts in oral epithelial dysplasia and oral squamous cell carcinoma |
title_full | Immunohistochemical evaluation of myofibroblasts in oral epithelial dysplasia and oral squamous cell carcinoma |
title_fullStr | Immunohistochemical evaluation of myofibroblasts in oral epithelial dysplasia and oral squamous cell carcinoma |
title_full_unstemmed | Immunohistochemical evaluation of myofibroblasts in oral epithelial dysplasia and oral squamous cell carcinoma |
title_short | Immunohistochemical evaluation of myofibroblasts in oral epithelial dysplasia and oral squamous cell carcinoma |
title_sort | immunohistochemical evaluation of myofibroblasts in oral epithelial dysplasia and oral squamous cell carcinoma |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8859618/ https://www.ncbi.nlm.nih.gov/pubmed/35281169 http://dx.doi.org/10.4103/jomfp.jomfp_505_20 |
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