Interaction of Periplasmic Fab Production and Intracellular Redox Balance in Escherichia coli Affects Product Yield

[Image: see text] Antibody fragments such as Fab’s require the formation of disulfide bonds to achieve a proper folding state. During their recombinant, periplasmic expression in Escherichia coli, oxidative folding is mediated by the DsbA/DsbB system in concert with ubiquinone. Thereby, overexpressi...

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Detalles Bibliográficos
Autores principales: Vazulka, Sophie, Schiavinato, Matteo, Wagenknecht, Martin, Cserjan-Puschmann, Monika, Striedner, Gerald
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2022
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8859853/
https://www.ncbi.nlm.nih.gov/pubmed/35041397
http://dx.doi.org/10.1021/acssynbio.1c00502

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8859853/
https://www.ncbi.nlm.nih.gov/pubmed/35041397
http://dx.doi.org/10.1021/acssynbio.1c00502

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