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ANKRD24 organizes TRIOBP to reinforce stereocilia insertion points
The stereocilia rootlet is a key structure in vertebrate hair cells, anchoring stereocilia firmly into the cell’s cuticular plate and protecting them from overstimulation. Using superresolution microscopy, we show that the ankyrin-repeat protein ANKRD24 concentrates at the stereocilia insertion poin...
Autores principales: | , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Rockefeller University Press
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8859912/ https://www.ncbi.nlm.nih.gov/pubmed/35175278 http://dx.doi.org/10.1083/jcb.202109134 |
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author | Krey, Jocelyn F. Liu, Chang Belyantseva, Inna A. Bateschell, Michael Dumont, Rachel A. Goldsmith, Jennifer Chatterjee, Paroma Morrill, Rachel S. Fedorov, Lev M. Foster, Sarah Kim, Jinkyung Nuttall, Alfred L. Jones, Sherri M. Choi, Dongseok Friedman, Thomas B. Ricci, Anthony J. Zhao, Bo Barr-Gillespie, Peter G. |
author_facet | Krey, Jocelyn F. Liu, Chang Belyantseva, Inna A. Bateschell, Michael Dumont, Rachel A. Goldsmith, Jennifer Chatterjee, Paroma Morrill, Rachel S. Fedorov, Lev M. Foster, Sarah Kim, Jinkyung Nuttall, Alfred L. Jones, Sherri M. Choi, Dongseok Friedman, Thomas B. Ricci, Anthony J. Zhao, Bo Barr-Gillespie, Peter G. |
author_sort | Krey, Jocelyn F. |
collection | PubMed |
description | The stereocilia rootlet is a key structure in vertebrate hair cells, anchoring stereocilia firmly into the cell’s cuticular plate and protecting them from overstimulation. Using superresolution microscopy, we show that the ankyrin-repeat protein ANKRD24 concentrates at the stereocilia insertion point, forming a ring at the junction between the lower and upper rootlets. Annular ANKRD24 continues into the lower rootlet, where it surrounds and binds TRIOBP-5, which itself bundles rootlet F-actin. TRIOBP-5 is mislocalized in Ankrd24(KO/KO) hair cells, and ANKRD24 no longer localizes with rootlets in mice lacking TRIOBP-5; exogenous DsRed–TRIOBP-5 restores endogenous ANKRD24 to rootlets in these mice. Ankrd24(KO/KO) mice show progressive hearing loss and diminished recovery of auditory function after noise damage, as well as increased susceptibility to overstimulation of the hair bundle. We propose that ANKRD24 bridges the apical plasma membrane with the lower rootlet, maintaining a normal distribution of TRIOBP-5. Together with TRIOBP-5, ANKRD24 organizes rootlets to enable hearing with long-term resilience. |
format | Online Article Text |
id | pubmed-8859912 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-88599122022-02-23 ANKRD24 organizes TRIOBP to reinforce stereocilia insertion points Krey, Jocelyn F. Liu, Chang Belyantseva, Inna A. Bateschell, Michael Dumont, Rachel A. Goldsmith, Jennifer Chatterjee, Paroma Morrill, Rachel S. Fedorov, Lev M. Foster, Sarah Kim, Jinkyung Nuttall, Alfred L. Jones, Sherri M. Choi, Dongseok Friedman, Thomas B. Ricci, Anthony J. Zhao, Bo Barr-Gillespie, Peter G. J Cell Biol Article The stereocilia rootlet is a key structure in vertebrate hair cells, anchoring stereocilia firmly into the cell’s cuticular plate and protecting them from overstimulation. Using superresolution microscopy, we show that the ankyrin-repeat protein ANKRD24 concentrates at the stereocilia insertion point, forming a ring at the junction between the lower and upper rootlets. Annular ANKRD24 continues into the lower rootlet, where it surrounds and binds TRIOBP-5, which itself bundles rootlet F-actin. TRIOBP-5 is mislocalized in Ankrd24(KO/KO) hair cells, and ANKRD24 no longer localizes with rootlets in mice lacking TRIOBP-5; exogenous DsRed–TRIOBP-5 restores endogenous ANKRD24 to rootlets in these mice. Ankrd24(KO/KO) mice show progressive hearing loss and diminished recovery of auditory function after noise damage, as well as increased susceptibility to overstimulation of the hair bundle. We propose that ANKRD24 bridges the apical plasma membrane with the lower rootlet, maintaining a normal distribution of TRIOBP-5. Together with TRIOBP-5, ANKRD24 organizes rootlets to enable hearing with long-term resilience. Rockefeller University Press 2022-02-17 /pmc/articles/PMC8859912/ /pubmed/35175278 http://dx.doi.org/10.1083/jcb.202109134 Text en © 2022 Krey et al. https://creativecommons.org/licenses/by/4.0/This article is available under a Creative Commons License (Attribution 4.0 International, as described at https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Krey, Jocelyn F. Liu, Chang Belyantseva, Inna A. Bateschell, Michael Dumont, Rachel A. Goldsmith, Jennifer Chatterjee, Paroma Morrill, Rachel S. Fedorov, Lev M. Foster, Sarah Kim, Jinkyung Nuttall, Alfred L. Jones, Sherri M. Choi, Dongseok Friedman, Thomas B. Ricci, Anthony J. Zhao, Bo Barr-Gillespie, Peter G. ANKRD24 organizes TRIOBP to reinforce stereocilia insertion points |
title | ANKRD24 organizes TRIOBP to reinforce stereocilia insertion points |
title_full | ANKRD24 organizes TRIOBP to reinforce stereocilia insertion points |
title_fullStr | ANKRD24 organizes TRIOBP to reinforce stereocilia insertion points |
title_full_unstemmed | ANKRD24 organizes TRIOBP to reinforce stereocilia insertion points |
title_short | ANKRD24 organizes TRIOBP to reinforce stereocilia insertion points |
title_sort | ankrd24 organizes triobp to reinforce stereocilia insertion points |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8859912/ https://www.ncbi.nlm.nih.gov/pubmed/35175278 http://dx.doi.org/10.1083/jcb.202109134 |
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