Cargando…
The P4 Study: Postpartum Maternal and Infant Faecal Microbiome 6 Months After Hypertensive Versus Normotensive Pregnancy
OBJECTIVE/HYPOTHESIS: To explore potential differences in faecal microbiome between women, and their infants, who had normotensive pregnancies (NP) and those who had a hypertensive pregnancy (HP), either gestational hypertension (GH) or preeclampsia (PE). METHODS: This is a sub study of P4 (Postpart...
Autores principales: | , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8860159/ https://www.ncbi.nlm.nih.gov/pubmed/35198457 http://dx.doi.org/10.3389/fcimb.2022.646165 |
_version_ | 1784654609348820992 |
---|---|
author | Susic, Daniella Frances Wang, Leanne Roberts, Lynne Margaret Bai, Michelle Gia, Andrew McGovern, Emily Jiang, Xiao-Tao Davis, Gregory K. El-Omar, Emad Henry, Amanda |
author_facet | Susic, Daniella Frances Wang, Leanne Roberts, Lynne Margaret Bai, Michelle Gia, Andrew McGovern, Emily Jiang, Xiao-Tao Davis, Gregory K. El-Omar, Emad Henry, Amanda |
author_sort | Susic, Daniella Frances |
collection | PubMed |
description | OBJECTIVE/HYPOTHESIS: To explore potential differences in faecal microbiome between women, and their infants, who had normotensive pregnancies (NP) and those who had a hypertensive pregnancy (HP), either gestational hypertension (GH) or preeclampsia (PE). METHODS: This is a sub study of P4 (Postpartum Physiology, Psychology, and Paediatrics Study) and includes 18 mother-infant pairs: 10 NP and 8 HP (HP as defined by blood pressure > 140/90mmHg; of which 6 had PE, and 2 GH), six months postpartum. The participating mothers collected stool samples from themselves and their infants. 16S rRNA V3-V4 amplicons were used to study the faecal microbiome. RESULTS: The sample of women and their infants were mostly primiparous (n =16) with vaginal birth (n = 14). At the time of faecal sampling 8 women were using hormonal contraception, and one HP woman remained on an antihypertensive. All women had blood pressure < 130/80mmHg, and 10 had high BMI (> 30). All infants had started solids, 8 were exclusively breastfed, 1 exclusively formula fed and 9 both. Three infants had been exposed to a course of antibiotics. Six months postpartum, there were no significant differences in alpha or beta diversity between the gut microbiota of HP and NP women (P > 0.05). However, a statistically significant difference was detected in alpha diversity between infants following HP and NP, with lower diversity levels in HP infants (P < 0.05). It was also found that at a genus and species level, the gut microbiota of HP women was enriched with Bifidobacterium and Bifidobacterium sp. and depleted in Barnesiella and Barnesiella intestinihominis when compared to NP women (P < 0.05). Similarly, the gut microbiota of infants born from HP was enriched in Streptococcus infantis and depleted in Sutterella, Sutterella sp., Bacteroides sp. and Clostridium aldenense compared to infants born from NP (P < 0.05). DISCUSSION: While our findings are at best preliminary, due to the very small sample size, they do suggest that the presence of hypertension in pregnancy may adversely affect the maternal microbiota postpartum, and that of their infants. Further analysis of postpartum microbiome data from future studies will be important to validate these early findings and provide further evidence about the changes in the microbiota in the offspring of women following hypertensive disorders of pregnancy (HDP), including possible links to the causes of long-term cardiovascular disease, the prevalence of which is increased in women who have experienced HDP. |
format | Online Article Text |
id | pubmed-8860159 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-88601592022-02-22 The P4 Study: Postpartum Maternal and Infant Faecal Microbiome 6 Months After Hypertensive Versus Normotensive Pregnancy Susic, Daniella Frances Wang, Leanne Roberts, Lynne Margaret Bai, Michelle Gia, Andrew McGovern, Emily Jiang, Xiao-Tao Davis, Gregory K. El-Omar, Emad Henry, Amanda Front Cell Infect Microbiol Cellular and Infection Microbiology OBJECTIVE/HYPOTHESIS: To explore potential differences in faecal microbiome between women, and their infants, who had normotensive pregnancies (NP) and those who had a hypertensive pregnancy (HP), either gestational hypertension (GH) or preeclampsia (PE). METHODS: This is a sub study of P4 (Postpartum Physiology, Psychology, and Paediatrics Study) and includes 18 mother-infant pairs: 10 NP and 8 HP (HP as defined by blood pressure > 140/90mmHg; of which 6 had PE, and 2 GH), six months postpartum. The participating mothers collected stool samples from themselves and their infants. 16S rRNA V3-V4 amplicons were used to study the faecal microbiome. RESULTS: The sample of women and their infants were mostly primiparous (n =16) with vaginal birth (n = 14). At the time of faecal sampling 8 women were using hormonal contraception, and one HP woman remained on an antihypertensive. All women had blood pressure < 130/80mmHg, and 10 had high BMI (> 30). All infants had started solids, 8 were exclusively breastfed, 1 exclusively formula fed and 9 both. Three infants had been exposed to a course of antibiotics. Six months postpartum, there were no significant differences in alpha or beta diversity between the gut microbiota of HP and NP women (P > 0.05). However, a statistically significant difference was detected in alpha diversity between infants following HP and NP, with lower diversity levels in HP infants (P < 0.05). It was also found that at a genus and species level, the gut microbiota of HP women was enriched with Bifidobacterium and Bifidobacterium sp. and depleted in Barnesiella and Barnesiella intestinihominis when compared to NP women (P < 0.05). Similarly, the gut microbiota of infants born from HP was enriched in Streptococcus infantis and depleted in Sutterella, Sutterella sp., Bacteroides sp. and Clostridium aldenense compared to infants born from NP (P < 0.05). DISCUSSION: While our findings are at best preliminary, due to the very small sample size, they do suggest that the presence of hypertension in pregnancy may adversely affect the maternal microbiota postpartum, and that of their infants. Further analysis of postpartum microbiome data from future studies will be important to validate these early findings and provide further evidence about the changes in the microbiota in the offspring of women following hypertensive disorders of pregnancy (HDP), including possible links to the causes of long-term cardiovascular disease, the prevalence of which is increased in women who have experienced HDP. Frontiers Media S.A. 2022-01-31 /pmc/articles/PMC8860159/ /pubmed/35198457 http://dx.doi.org/10.3389/fcimb.2022.646165 Text en Copyright © 2022 Susic, Wang, Roberts, Bai, Gia, McGovern, Jiang, Davis, El-Omar and Henry https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Cellular and Infection Microbiology Susic, Daniella Frances Wang, Leanne Roberts, Lynne Margaret Bai, Michelle Gia, Andrew McGovern, Emily Jiang, Xiao-Tao Davis, Gregory K. El-Omar, Emad Henry, Amanda The P4 Study: Postpartum Maternal and Infant Faecal Microbiome 6 Months After Hypertensive Versus Normotensive Pregnancy |
title | The P4 Study: Postpartum Maternal and Infant Faecal Microbiome 6 Months After Hypertensive Versus Normotensive Pregnancy |
title_full | The P4 Study: Postpartum Maternal and Infant Faecal Microbiome 6 Months After Hypertensive Versus Normotensive Pregnancy |
title_fullStr | The P4 Study: Postpartum Maternal and Infant Faecal Microbiome 6 Months After Hypertensive Versus Normotensive Pregnancy |
title_full_unstemmed | The P4 Study: Postpartum Maternal and Infant Faecal Microbiome 6 Months After Hypertensive Versus Normotensive Pregnancy |
title_short | The P4 Study: Postpartum Maternal and Infant Faecal Microbiome 6 Months After Hypertensive Versus Normotensive Pregnancy |
title_sort | p4 study: postpartum maternal and infant faecal microbiome 6 months after hypertensive versus normotensive pregnancy |
topic | Cellular and Infection Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8860159/ https://www.ncbi.nlm.nih.gov/pubmed/35198457 http://dx.doi.org/10.3389/fcimb.2022.646165 |
work_keys_str_mv | AT susicdaniellafrances thep4studypostpartummaternalandinfantfaecalmicrobiome6monthsafterhypertensiveversusnormotensivepregnancy AT wangleanne thep4studypostpartummaternalandinfantfaecalmicrobiome6monthsafterhypertensiveversusnormotensivepregnancy AT robertslynnemargaret thep4studypostpartummaternalandinfantfaecalmicrobiome6monthsafterhypertensiveversusnormotensivepregnancy AT baimichelle thep4studypostpartummaternalandinfantfaecalmicrobiome6monthsafterhypertensiveversusnormotensivepregnancy AT giaandrew thep4studypostpartummaternalandinfantfaecalmicrobiome6monthsafterhypertensiveversusnormotensivepregnancy AT mcgovernemily thep4studypostpartummaternalandinfantfaecalmicrobiome6monthsafterhypertensiveversusnormotensivepregnancy AT jiangxiaotao thep4studypostpartummaternalandinfantfaecalmicrobiome6monthsafterhypertensiveversusnormotensivepregnancy AT davisgregoryk thep4studypostpartummaternalandinfantfaecalmicrobiome6monthsafterhypertensiveversusnormotensivepregnancy AT elomaremad thep4studypostpartummaternalandinfantfaecalmicrobiome6monthsafterhypertensiveversusnormotensivepregnancy AT henryamanda thep4studypostpartummaternalandinfantfaecalmicrobiome6monthsafterhypertensiveversusnormotensivepregnancy AT susicdaniellafrances p4studypostpartummaternalandinfantfaecalmicrobiome6monthsafterhypertensiveversusnormotensivepregnancy AT wangleanne p4studypostpartummaternalandinfantfaecalmicrobiome6monthsafterhypertensiveversusnormotensivepregnancy AT robertslynnemargaret p4studypostpartummaternalandinfantfaecalmicrobiome6monthsafterhypertensiveversusnormotensivepregnancy AT baimichelle p4studypostpartummaternalandinfantfaecalmicrobiome6monthsafterhypertensiveversusnormotensivepregnancy AT giaandrew p4studypostpartummaternalandinfantfaecalmicrobiome6monthsafterhypertensiveversusnormotensivepregnancy AT mcgovernemily p4studypostpartummaternalandinfantfaecalmicrobiome6monthsafterhypertensiveversusnormotensivepregnancy AT jiangxiaotao p4studypostpartummaternalandinfantfaecalmicrobiome6monthsafterhypertensiveversusnormotensivepregnancy AT davisgregoryk p4studypostpartummaternalandinfantfaecalmicrobiome6monthsafterhypertensiveversusnormotensivepregnancy AT elomaremad p4studypostpartummaternalandinfantfaecalmicrobiome6monthsafterhypertensiveversusnormotensivepregnancy AT henryamanda p4studypostpartummaternalandinfantfaecalmicrobiome6monthsafterhypertensiveversusnormotensivepregnancy |