Cargando…
A liquid fraction of extracellular matrix inhibits glioma cell viability in vitro and in vivo
Suppressive effects of extracellular matrix (ECM) upon various cancers have been reported. Glioblastoma multiforme has poor prognosis and new therapies are desired. This work investigated the effects of a saline-soluble fraction of urinary bladder ECM (ECM-SF) upon glioma cells. Viability at 24 hour...
Autores principales: | , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Impact Journals LLC
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8860176/ https://www.ncbi.nlm.nih.gov/pubmed/35198102 http://dx.doi.org/10.18632/oncotarget.28203 |
_version_ | 1784654612810170368 |
---|---|
author | Murdock, Mark H. Hussey, George S. Chang, Jordan T. Hill, Ryan C. Nascari, David G. Rao, Aparna V. Hansen, Kirk C. Foley, Lesley M. Hitchens, T. Kevin Amankulor, Nduka M. Badylak, Stephen F. |
author_facet | Murdock, Mark H. Hussey, George S. Chang, Jordan T. Hill, Ryan C. Nascari, David G. Rao, Aparna V. Hansen, Kirk C. Foley, Lesley M. Hitchens, T. Kevin Amankulor, Nduka M. Badylak, Stephen F. |
author_sort | Murdock, Mark H. |
collection | PubMed |
description | Suppressive effects of extracellular matrix (ECM) upon various cancers have been reported. Glioblastoma multiforme has poor prognosis and new therapies are desired. This work investigated the effects of a saline-soluble fraction of urinary bladder ECM (ECM-SF) upon glioma cells. Viability at 24 hours in 1, 5, or 10 mg/mL ECM-SF-spiked media was evaluated in primary glioma cells (0319, 1015, 1119), glioma cell lines (A172, T98G, U87MG, C6), and brain cell lines (HCN-2, HMC3). Viability universally decreased at 5 and 10 mg/mL with U87MG, HCN-2, and HCM3 being least sensitive. Apoptosis in 0319 and 1119 cells was confirmed via NucView 488. Bi-weekly intravenous injection of ECM-SF (120 mg/kg) for 10 weeks in Sprague-Dawley rats did not affect weight, temperature, complete blood count, or multi-organ histology (N = 5). Intratumoral injection of ECM-SF (10 uL of 30 mg/mL) at weeks 2–4 post C6 inoculation in Wistar rats increased median survival from 24.5 to 51 days (hazard ratio for death 0.22) and decreased average tumor volume at time of death from 349 mm(3) to 90 mm(3) over 10 weeks (N = 6). Mass spectrometry identified 2,562 protein species in ECM-SF, parent ECM, and originating tissue. These results demonstrate the suppressive effects of ECM on glioma and warrant further study. |
format | Online Article Text |
id | pubmed-8860176 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Impact Journals LLC |
record_format | MEDLINE/PubMed |
spelling | pubmed-88601762022-02-22 A liquid fraction of extracellular matrix inhibits glioma cell viability in vitro and in vivo Murdock, Mark H. Hussey, George S. Chang, Jordan T. Hill, Ryan C. Nascari, David G. Rao, Aparna V. Hansen, Kirk C. Foley, Lesley M. Hitchens, T. Kevin Amankulor, Nduka M. Badylak, Stephen F. Oncotarget Research Paper Suppressive effects of extracellular matrix (ECM) upon various cancers have been reported. Glioblastoma multiforme has poor prognosis and new therapies are desired. This work investigated the effects of a saline-soluble fraction of urinary bladder ECM (ECM-SF) upon glioma cells. Viability at 24 hours in 1, 5, or 10 mg/mL ECM-SF-spiked media was evaluated in primary glioma cells (0319, 1015, 1119), glioma cell lines (A172, T98G, U87MG, C6), and brain cell lines (HCN-2, HMC3). Viability universally decreased at 5 and 10 mg/mL with U87MG, HCN-2, and HCM3 being least sensitive. Apoptosis in 0319 and 1119 cells was confirmed via NucView 488. Bi-weekly intravenous injection of ECM-SF (120 mg/kg) for 10 weeks in Sprague-Dawley rats did not affect weight, temperature, complete blood count, or multi-organ histology (N = 5). Intratumoral injection of ECM-SF (10 uL of 30 mg/mL) at weeks 2–4 post C6 inoculation in Wistar rats increased median survival from 24.5 to 51 days (hazard ratio for death 0.22) and decreased average tumor volume at time of death from 349 mm(3) to 90 mm(3) over 10 weeks (N = 6). Mass spectrometry identified 2,562 protein species in ECM-SF, parent ECM, and originating tissue. These results demonstrate the suppressive effects of ECM on glioma and warrant further study. Impact Journals LLC 2022-02-21 /pmc/articles/PMC8860176/ /pubmed/35198102 http://dx.doi.org/10.18632/oncotarget.28203 Text en Copyright: © 2022 Murdock et al. https://creativecommons.org/licenses/by/3.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/3.0/) (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Paper Murdock, Mark H. Hussey, George S. Chang, Jordan T. Hill, Ryan C. Nascari, David G. Rao, Aparna V. Hansen, Kirk C. Foley, Lesley M. Hitchens, T. Kevin Amankulor, Nduka M. Badylak, Stephen F. A liquid fraction of extracellular matrix inhibits glioma cell viability in vitro and in vivo |
title | A liquid fraction of extracellular matrix inhibits glioma cell viability in vitro and in vivo
|
title_full | A liquid fraction of extracellular matrix inhibits glioma cell viability in vitro and in vivo
|
title_fullStr | A liquid fraction of extracellular matrix inhibits glioma cell viability in vitro and in vivo
|
title_full_unstemmed | A liquid fraction of extracellular matrix inhibits glioma cell viability in vitro and in vivo
|
title_short | A liquid fraction of extracellular matrix inhibits glioma cell viability in vitro and in vivo
|
title_sort | liquid fraction of extracellular matrix inhibits glioma cell viability in vitro and in vivo |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8860176/ https://www.ncbi.nlm.nih.gov/pubmed/35198102 http://dx.doi.org/10.18632/oncotarget.28203 |
work_keys_str_mv | AT murdockmarkh aliquidfractionofextracellularmatrixinhibitsgliomacellviabilityinvitroandinvivo AT husseygeorges aliquidfractionofextracellularmatrixinhibitsgliomacellviabilityinvitroandinvivo AT changjordant aliquidfractionofextracellularmatrixinhibitsgliomacellviabilityinvitroandinvivo AT hillryanc aliquidfractionofextracellularmatrixinhibitsgliomacellviabilityinvitroandinvivo AT nascaridavidg aliquidfractionofextracellularmatrixinhibitsgliomacellviabilityinvitroandinvivo AT raoaparnav aliquidfractionofextracellularmatrixinhibitsgliomacellviabilityinvitroandinvivo AT hansenkirkc aliquidfractionofextracellularmatrixinhibitsgliomacellviabilityinvitroandinvivo AT foleylesleym aliquidfractionofextracellularmatrixinhibitsgliomacellviabilityinvitroandinvivo AT hitchenstkevin aliquidfractionofextracellularmatrixinhibitsgliomacellviabilityinvitroandinvivo AT amankulorndukam aliquidfractionofextracellularmatrixinhibitsgliomacellviabilityinvitroandinvivo AT badylakstephenf aliquidfractionofextracellularmatrixinhibitsgliomacellviabilityinvitroandinvivo AT murdockmarkh liquidfractionofextracellularmatrixinhibitsgliomacellviabilityinvitroandinvivo AT husseygeorges liquidfractionofextracellularmatrixinhibitsgliomacellviabilityinvitroandinvivo AT changjordant liquidfractionofextracellularmatrixinhibitsgliomacellviabilityinvitroandinvivo AT hillryanc liquidfractionofextracellularmatrixinhibitsgliomacellviabilityinvitroandinvivo AT nascaridavidg liquidfractionofextracellularmatrixinhibitsgliomacellviabilityinvitroandinvivo AT raoaparnav liquidfractionofextracellularmatrixinhibitsgliomacellviabilityinvitroandinvivo AT hansenkirkc liquidfractionofextracellularmatrixinhibitsgliomacellviabilityinvitroandinvivo AT foleylesleym liquidfractionofextracellularmatrixinhibitsgliomacellviabilityinvitroandinvivo AT hitchenstkevin liquidfractionofextracellularmatrixinhibitsgliomacellviabilityinvitroandinvivo AT amankulorndukam liquidfractionofextracellularmatrixinhibitsgliomacellviabilityinvitroandinvivo AT badylakstephenf liquidfractionofextracellularmatrixinhibitsgliomacellviabilityinvitroandinvivo |