A cell-based system combined with flow cytometry to evaluate antibody responses against SARS-CoV-2 transmembrane proteins in patients with COVID-19

This protocol describes a flow cytometry approach to evaluate antibody responses against SARS-CoV-2 transmembrane proteins in COVID-19-positive patient sera samples without the need of specific laboratory facilities for viral infection. We developed a human-cell-based system using spike-expressing H...

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Autores principales: Martin, Sophie, Jégou, Gwénaële, Nicolas, Aurore, Le Gallo, Matthieu, Chevet, Éric, Godey, Florence, Avril, Tony
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8860709/
https://www.ncbi.nlm.nih.gov/pubmed/35287269
http://dx.doi.org/10.1016/j.xpro.2022.101229
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author Martin, Sophie
Jégou, Gwénaële
Nicolas, Aurore
Le Gallo, Matthieu
Chevet, Éric
Godey, Florence
Avril, Tony
author_facet Martin, Sophie
Jégou, Gwénaële
Nicolas, Aurore
Le Gallo, Matthieu
Chevet, Éric
Godey, Florence
Avril, Tony
author_sort Martin, Sophie
collection PubMed
description This protocol describes a flow cytometry approach to evaluate antibody responses against SARS-CoV-2 transmembrane proteins in COVID-19-positive patient sera samples without the need of specific laboratory facilities for viral infection. We developed a human-cell-based system using spike-expressing HEK293T cells that mimics membrane insertion and N-glycosylation of viral integral membrane proteins in host cells. This assay represents a powerful tool to test antibody responses against SARS-CoV-2 variants and vaccine effectiveness. For complete details on the use and execution of this protocol, please refer to Martin et al. (2021).
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spelling pubmed-88607092022-02-22 A cell-based system combined with flow cytometry to evaluate antibody responses against SARS-CoV-2 transmembrane proteins in patients with COVID-19 Martin, Sophie Jégou, Gwénaële Nicolas, Aurore Le Gallo, Matthieu Chevet, Éric Godey, Florence Avril, Tony STAR Protoc Protocol This protocol describes a flow cytometry approach to evaluate antibody responses against SARS-CoV-2 transmembrane proteins in COVID-19-positive patient sera samples without the need of specific laboratory facilities for viral infection. We developed a human-cell-based system using spike-expressing HEK293T cells that mimics membrane insertion and N-glycosylation of viral integral membrane proteins in host cells. This assay represents a powerful tool to test antibody responses against SARS-CoV-2 variants and vaccine effectiveness. For complete details on the use and execution of this protocol, please refer to Martin et al. (2021). Elsevier 2022-02-22 /pmc/articles/PMC8860709/ /pubmed/35287269 http://dx.doi.org/10.1016/j.xpro.2022.101229 Text en © 2022 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Martin, Sophie
Jégou, Gwénaële
Nicolas, Aurore
Le Gallo, Matthieu
Chevet, Éric
Godey, Florence
Avril, Tony
A cell-based system combined with flow cytometry to evaluate antibody responses against SARS-CoV-2 transmembrane proteins in patients with COVID-19
title A cell-based system combined with flow cytometry to evaluate antibody responses against SARS-CoV-2 transmembrane proteins in patients with COVID-19
title_full A cell-based system combined with flow cytometry to evaluate antibody responses against SARS-CoV-2 transmembrane proteins in patients with COVID-19
title_fullStr A cell-based system combined with flow cytometry to evaluate antibody responses against SARS-CoV-2 transmembrane proteins in patients with COVID-19
title_full_unstemmed A cell-based system combined with flow cytometry to evaluate antibody responses against SARS-CoV-2 transmembrane proteins in patients with COVID-19
title_short A cell-based system combined with flow cytometry to evaluate antibody responses against SARS-CoV-2 transmembrane proteins in patients with COVID-19
title_sort cell-based system combined with flow cytometry to evaluate antibody responses against sars-cov-2 transmembrane proteins in patients with covid-19
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8860709/
https://www.ncbi.nlm.nih.gov/pubmed/35287269
http://dx.doi.org/10.1016/j.xpro.2022.101229
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