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Changes in Brassica oleracea Leaves Infected With Xanthomonas campestris pv. campestris by Proteomics Analysis

Understanding plant’s response mechanisms against pathogenesis is fundamental for the development of resistant crop varieties and more productive agriculture. In this regard, “omic” approaches are heralded as valuable technologies. In this work, combining isobaric tags for relative and absolute quan...

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Autores principales: Tortosa, María, Velasco, Pablo, Rodríguez, Víctor Manuel, Cartea, María Elena
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8860909/
https://www.ncbi.nlm.nih.gov/pubmed/35211128
http://dx.doi.org/10.3389/fpls.2021.781984
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author Tortosa, María
Velasco, Pablo
Rodríguez, Víctor Manuel
Cartea, María Elena
author_facet Tortosa, María
Velasco, Pablo
Rodríguez, Víctor Manuel
Cartea, María Elena
author_sort Tortosa, María
collection PubMed
description Understanding plant’s response mechanisms against pathogenesis is fundamental for the development of resistant crop varieties and more productive agriculture. In this regard, “omic” approaches are heralded as valuable technologies. In this work, combining isobaric tags for relative and absolute quantification (iTRAQ) technology with mass spectrometry, the proteomes from leaves of Brassica oleracea plants infected with Xanthomonas campestris pv. campestris (Xcc), and control plants at two different post-infection times were compared. Stronger proteomic changes were obtained at 12 days post-infection in comparison with 3 days. The responses observed involved different cell processes, from primary metabolism, such as photosynthesis or photorespiration, to other complex processes such as redox homeostasis, hormone signaling, or defense mechanisms. Most of the proteins decreased in the earlier response were involved in energetic metabolism, whereas later response was characterized by a recovery of primary metabolism. Furthermore, our results indicated that proteolysis machinery and reactive oxygen species (ROS) homeostasis could be key processes during this plant–pathogen interaction. Current data provide new insights into molecular mechanisms that may be involved in defense responses of B. oleracea to Xcc.
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spelling pubmed-88609092022-02-23 Changes in Brassica oleracea Leaves Infected With Xanthomonas campestris pv. campestris by Proteomics Analysis Tortosa, María Velasco, Pablo Rodríguez, Víctor Manuel Cartea, María Elena Front Plant Sci Plant Science Understanding plant’s response mechanisms against pathogenesis is fundamental for the development of resistant crop varieties and more productive agriculture. In this regard, “omic” approaches are heralded as valuable technologies. In this work, combining isobaric tags for relative and absolute quantification (iTRAQ) technology with mass spectrometry, the proteomes from leaves of Brassica oleracea plants infected with Xanthomonas campestris pv. campestris (Xcc), and control plants at two different post-infection times were compared. Stronger proteomic changes were obtained at 12 days post-infection in comparison with 3 days. The responses observed involved different cell processes, from primary metabolism, such as photosynthesis or photorespiration, to other complex processes such as redox homeostasis, hormone signaling, or defense mechanisms. Most of the proteins decreased in the earlier response were involved in energetic metabolism, whereas later response was characterized by a recovery of primary metabolism. Furthermore, our results indicated that proteolysis machinery and reactive oxygen species (ROS) homeostasis could be key processes during this plant–pathogen interaction. Current data provide new insights into molecular mechanisms that may be involved in defense responses of B. oleracea to Xcc. Frontiers Media S.A. 2022-02-08 /pmc/articles/PMC8860909/ /pubmed/35211128 http://dx.doi.org/10.3389/fpls.2021.781984 Text en Copyright © 2022 Tortosa, Velasco, Rodríguez and Cartea. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Tortosa, María
Velasco, Pablo
Rodríguez, Víctor Manuel
Cartea, María Elena
Changes in Brassica oleracea Leaves Infected With Xanthomonas campestris pv. campestris by Proteomics Analysis
title Changes in Brassica oleracea Leaves Infected With Xanthomonas campestris pv. campestris by Proteomics Analysis
title_full Changes in Brassica oleracea Leaves Infected With Xanthomonas campestris pv. campestris by Proteomics Analysis
title_fullStr Changes in Brassica oleracea Leaves Infected With Xanthomonas campestris pv. campestris by Proteomics Analysis
title_full_unstemmed Changes in Brassica oleracea Leaves Infected With Xanthomonas campestris pv. campestris by Proteomics Analysis
title_short Changes in Brassica oleracea Leaves Infected With Xanthomonas campestris pv. campestris by Proteomics Analysis
title_sort changes in brassica oleracea leaves infected with xanthomonas campestris pv. campestris by proteomics analysis
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8860909/
https://www.ncbi.nlm.nih.gov/pubmed/35211128
http://dx.doi.org/10.3389/fpls.2021.781984
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