Cargando…
An Improved and Simplified Propagation System for Pollen-Free Sugi (Cryptomeria japonica) via Somatic Embryogenesis
Sugi (Japanese cedar, Cryptomeria japonica) is the most important forestry tree species in Japan, covering 44% of the total artificial forest area. Large amounts of pollen released from these forests each spring cause allergic reactions in approximately 40% of the population, which are a serious soc...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8861444/ https://www.ncbi.nlm.nih.gov/pubmed/35211140 http://dx.doi.org/10.3389/fpls.2022.825340 |
_version_ | 1784654888553152512 |
---|---|
author | Maruyama, Tsuyoshi E. Tsuruta, Momi Ueno, Saneyoshi Kawakami, Kiyohisa Bamba, Yukiko Moriguchi, Yoshinari |
author_facet | Maruyama, Tsuyoshi E. Tsuruta, Momi Ueno, Saneyoshi Kawakami, Kiyohisa Bamba, Yukiko Moriguchi, Yoshinari |
author_sort | Maruyama, Tsuyoshi E. |
collection | PubMed |
description | Sugi (Japanese cedar, Cryptomeria japonica) is the most important forestry tree species in Japan, covering 44% of the total artificial forest area. Large amounts of pollen released from these forests each spring cause allergic reactions in approximately 40% of the population, which are a serious social and public health problem in Japan. As a countermeasure, there is an urgent need to reforest using male-sterile plants (MSPs; pollen-free plants); however, the production of MSPs via conventional methods is inefficient, time consuming, and requires considerable resources in terms of labor and space. In the present paper, we described an improved and simplified methodology for the efficient propagation of pollen-free Japanese cedar, combining the use of genetic markers (marker-assisted selection or marker-aided selection) for the early selection of male-sterile genotypes and the use of somatic embryogenesis (SE) for the clonal mass propagation of seedlings. We describe all the stages involved in the production process of somatic seedlings. Our results demonstrated that this methodology easily and efficiently produces MSPs with a discrimination rate of 100% in a short period of time. Production of 243.6 ± 163.6 cotyledonary embryos per plate, somatic embryo germination, and plantlet conversion frequencies of 87.1 ± 11.9% and 84.8 ± 12.6%, respectively, and a 77.6 ± 12.1% survival rate after ex vitro acclimatization was achieved. Moreover, we also describe an easy method for the collection of somatic embryos prior to germination, as well as an efficient and practical method for their storage at 5°C. Finally, a representative schedule for the propagation of pollen-free sugi somatic seedlings is presented as a reference for practical uses. This methodology will definitively help to accelerate the production of C. japonica MSPs across Japan. |
format | Online Article Text |
id | pubmed-8861444 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-88614442022-02-23 An Improved and Simplified Propagation System for Pollen-Free Sugi (Cryptomeria japonica) via Somatic Embryogenesis Maruyama, Tsuyoshi E. Tsuruta, Momi Ueno, Saneyoshi Kawakami, Kiyohisa Bamba, Yukiko Moriguchi, Yoshinari Front Plant Sci Plant Science Sugi (Japanese cedar, Cryptomeria japonica) is the most important forestry tree species in Japan, covering 44% of the total artificial forest area. Large amounts of pollen released from these forests each spring cause allergic reactions in approximately 40% of the population, which are a serious social and public health problem in Japan. As a countermeasure, there is an urgent need to reforest using male-sterile plants (MSPs; pollen-free plants); however, the production of MSPs via conventional methods is inefficient, time consuming, and requires considerable resources in terms of labor and space. In the present paper, we described an improved and simplified methodology for the efficient propagation of pollen-free Japanese cedar, combining the use of genetic markers (marker-assisted selection or marker-aided selection) for the early selection of male-sterile genotypes and the use of somatic embryogenesis (SE) for the clonal mass propagation of seedlings. We describe all the stages involved in the production process of somatic seedlings. Our results demonstrated that this methodology easily and efficiently produces MSPs with a discrimination rate of 100% in a short period of time. Production of 243.6 ± 163.6 cotyledonary embryos per plate, somatic embryo germination, and plantlet conversion frequencies of 87.1 ± 11.9% and 84.8 ± 12.6%, respectively, and a 77.6 ± 12.1% survival rate after ex vitro acclimatization was achieved. Moreover, we also describe an easy method for the collection of somatic embryos prior to germination, as well as an efficient and practical method for their storage at 5°C. Finally, a representative schedule for the propagation of pollen-free sugi somatic seedlings is presented as a reference for practical uses. This methodology will definitively help to accelerate the production of C. japonica MSPs across Japan. Frontiers Media S.A. 2022-02-08 /pmc/articles/PMC8861444/ /pubmed/35211140 http://dx.doi.org/10.3389/fpls.2022.825340 Text en Copyright © 2022 Maruyama, Tsuruta, Ueno, Kawakami, Bamba and Moriguchi. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Plant Science Maruyama, Tsuyoshi E. Tsuruta, Momi Ueno, Saneyoshi Kawakami, Kiyohisa Bamba, Yukiko Moriguchi, Yoshinari An Improved and Simplified Propagation System for Pollen-Free Sugi (Cryptomeria japonica) via Somatic Embryogenesis |
title | An Improved and Simplified Propagation System for Pollen-Free Sugi (Cryptomeria japonica) via Somatic Embryogenesis |
title_full | An Improved and Simplified Propagation System for Pollen-Free Sugi (Cryptomeria japonica) via Somatic Embryogenesis |
title_fullStr | An Improved and Simplified Propagation System for Pollen-Free Sugi (Cryptomeria japonica) via Somatic Embryogenesis |
title_full_unstemmed | An Improved and Simplified Propagation System for Pollen-Free Sugi (Cryptomeria japonica) via Somatic Embryogenesis |
title_short | An Improved and Simplified Propagation System for Pollen-Free Sugi (Cryptomeria japonica) via Somatic Embryogenesis |
title_sort | improved and simplified propagation system for pollen-free sugi (cryptomeria japonica) via somatic embryogenesis |
topic | Plant Science |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8861444/ https://www.ncbi.nlm.nih.gov/pubmed/35211140 http://dx.doi.org/10.3389/fpls.2022.825340 |
work_keys_str_mv | AT maruyamatsuyoshie animprovedandsimplifiedpropagationsystemforpollenfreesugicryptomeriajaponicaviasomaticembryogenesis AT tsurutamomi animprovedandsimplifiedpropagationsystemforpollenfreesugicryptomeriajaponicaviasomaticembryogenesis AT uenosaneyoshi animprovedandsimplifiedpropagationsystemforpollenfreesugicryptomeriajaponicaviasomaticembryogenesis AT kawakamikiyohisa animprovedandsimplifiedpropagationsystemforpollenfreesugicryptomeriajaponicaviasomaticembryogenesis AT bambayukiko animprovedandsimplifiedpropagationsystemforpollenfreesugicryptomeriajaponicaviasomaticembryogenesis AT moriguchiyoshinari animprovedandsimplifiedpropagationsystemforpollenfreesugicryptomeriajaponicaviasomaticembryogenesis AT maruyamatsuyoshie improvedandsimplifiedpropagationsystemforpollenfreesugicryptomeriajaponicaviasomaticembryogenesis AT tsurutamomi improvedandsimplifiedpropagationsystemforpollenfreesugicryptomeriajaponicaviasomaticembryogenesis AT uenosaneyoshi improvedandsimplifiedpropagationsystemforpollenfreesugicryptomeriajaponicaviasomaticembryogenesis AT kawakamikiyohisa improvedandsimplifiedpropagationsystemforpollenfreesugicryptomeriajaponicaviasomaticembryogenesis AT bambayukiko improvedandsimplifiedpropagationsystemforpollenfreesugicryptomeriajaponicaviasomaticembryogenesis AT moriguchiyoshinari improvedandsimplifiedpropagationsystemforpollenfreesugicryptomeriajaponicaviasomaticembryogenesis |