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A plant receptor domain with functional analogies to animal malectin disables ER stress responses upon infection

Malectins from the oligosaccharyltransferase (OST) complex in the endoplasmic reticulum (ER) of animal cells are involved in ER quality control and contribute to the Unfolded Protein Response (UPR). Malectins are not found in plant cells, but malectin-like domains (MLDs) are constituents of many mem...

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Autores principales: Giordano, Laïla, Allasia, Valérie, Cremades, Alexandra, Hok, Sophie, Panabières, Franck, Bailly-Maître, Béatrice, Keller, Harald
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8861646/
https://www.ncbi.nlm.nih.gov/pubmed/35243239
http://dx.doi.org/10.1016/j.isci.2022.103877
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author Giordano, Laïla
Allasia, Valérie
Cremades, Alexandra
Hok, Sophie
Panabières, Franck
Bailly-Maître, Béatrice
Keller, Harald
author_facet Giordano, Laïla
Allasia, Valérie
Cremades, Alexandra
Hok, Sophie
Panabières, Franck
Bailly-Maître, Béatrice
Keller, Harald
author_sort Giordano, Laïla
collection PubMed
description Malectins from the oligosaccharyltransferase (OST) complex in the endoplasmic reticulum (ER) of animal cells are involved in ER quality control and contribute to the Unfolded Protein Response (UPR). Malectins are not found in plant cells, but malectin-like domains (MLDs) are constituents of many membrane-bound receptors. In Arabidopsis thaliana, the MLD-containing receptor IOS1 promotes successful infection by filamentous plant pathogens. We show that the MLD of its exodomain retains IOS1 in the ER of plant cells and attenuates the infection-induced UPR. Expression of the MLD in the ios1-1 knockout background is sufficient to complement infection-related phenotypes of the mutant, such as increased UPR and reduced disease susceptibility. IOS1 interacts with the ER membrane-associated ribophorin HAP6 from the OST complex, and hap6 mutants show decreased pathogen-responsive UPR and increased disease susceptibility. Altogether, this study revealed a previously uncharacterized role of a plant receptor domain in the regulation of ER stress during infection.
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spelling pubmed-88616462022-03-02 A plant receptor domain with functional analogies to animal malectin disables ER stress responses upon infection Giordano, Laïla Allasia, Valérie Cremades, Alexandra Hok, Sophie Panabières, Franck Bailly-Maître, Béatrice Keller, Harald iScience Article Malectins from the oligosaccharyltransferase (OST) complex in the endoplasmic reticulum (ER) of animal cells are involved in ER quality control and contribute to the Unfolded Protein Response (UPR). Malectins are not found in plant cells, but malectin-like domains (MLDs) are constituents of many membrane-bound receptors. In Arabidopsis thaliana, the MLD-containing receptor IOS1 promotes successful infection by filamentous plant pathogens. We show that the MLD of its exodomain retains IOS1 in the ER of plant cells and attenuates the infection-induced UPR. Expression of the MLD in the ios1-1 knockout background is sufficient to complement infection-related phenotypes of the mutant, such as increased UPR and reduced disease susceptibility. IOS1 interacts with the ER membrane-associated ribophorin HAP6 from the OST complex, and hap6 mutants show decreased pathogen-responsive UPR and increased disease susceptibility. Altogether, this study revealed a previously uncharacterized role of a plant receptor domain in the regulation of ER stress during infection. Elsevier 2022-02-05 /pmc/articles/PMC8861646/ /pubmed/35243239 http://dx.doi.org/10.1016/j.isci.2022.103877 Text en © 2022 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Article
Giordano, Laïla
Allasia, Valérie
Cremades, Alexandra
Hok, Sophie
Panabières, Franck
Bailly-Maître, Béatrice
Keller, Harald
A plant receptor domain with functional analogies to animal malectin disables ER stress responses upon infection
title A plant receptor domain with functional analogies to animal malectin disables ER stress responses upon infection
title_full A plant receptor domain with functional analogies to animal malectin disables ER stress responses upon infection
title_fullStr A plant receptor domain with functional analogies to animal malectin disables ER stress responses upon infection
title_full_unstemmed A plant receptor domain with functional analogies to animal malectin disables ER stress responses upon infection
title_short A plant receptor domain with functional analogies to animal malectin disables ER stress responses upon infection
title_sort plant receptor domain with functional analogies to animal malectin disables er stress responses upon infection
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8861646/
https://www.ncbi.nlm.nih.gov/pubmed/35243239
http://dx.doi.org/10.1016/j.isci.2022.103877
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