scGR-seq: Integrated analysis of glycan and RNA in single cells

Glycans are structurally diverse molecules found on the surface of living cells. The protocol details a system developed for combined analysis of glycan and RNA in single cells (scGR-seq) using human induced pluripotent stem cells (hiPSCs) and hiPSC-derived neural progenitor cells (NPCs). scGR-seq c...

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Detalles Bibliográficos
Autores principales: Odaka, Haruki, Ozaki, Haruka, Tateno, Hiroaki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8861808/
https://www.ncbi.nlm.nih.gov/pubmed/35243371
http://dx.doi.org/10.1016/j.xpro.2022.101179
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author Odaka, Haruki
Ozaki, Haruka
Tateno, Hiroaki
author_facet Odaka, Haruki
Ozaki, Haruka
Tateno, Hiroaki
author_sort Odaka, Haruki
collection PubMed
description Glycans are structurally diverse molecules found on the surface of living cells. The protocol details a system developed for combined analysis of glycan and RNA in single cells (scGR-seq) using human induced pluripotent stem cells (hiPSCs) and hiPSC-derived neural progenitor cells (NPCs). scGR-seq consists of DNA-barcoded lectin-based glycan profiling by sequencing (scGlycan-seq) and single-cell transcriptome profiling (scRNA-seq). scGR-seq will be an essential technique to delineate the cellular heterogeneity of glycans across multicellular systems. For complete details on the use and execution of this profile, please refer to Minoshima et al. (2021).
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spelling pubmed-88618082022-03-02 scGR-seq: Integrated analysis of glycan and RNA in single cells Odaka, Haruki Ozaki, Haruka Tateno, Hiroaki STAR Protoc Protocol Glycans are structurally diverse molecules found on the surface of living cells. The protocol details a system developed for combined analysis of glycan and RNA in single cells (scGR-seq) using human induced pluripotent stem cells (hiPSCs) and hiPSC-derived neural progenitor cells (NPCs). scGR-seq consists of DNA-barcoded lectin-based glycan profiling by sequencing (scGlycan-seq) and single-cell transcriptome profiling (scRNA-seq). scGR-seq will be an essential technique to delineate the cellular heterogeneity of glycans across multicellular systems. For complete details on the use and execution of this profile, please refer to Minoshima et al. (2021). Elsevier 2022-02-18 /pmc/articles/PMC8861808/ /pubmed/35243371 http://dx.doi.org/10.1016/j.xpro.2022.101179 Text en © 2022 The Author(s) https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Protocol
Odaka, Haruki
Ozaki, Haruka
Tateno, Hiroaki
scGR-seq: Integrated analysis of glycan and RNA in single cells
title scGR-seq: Integrated analysis of glycan and RNA in single cells
title_full scGR-seq: Integrated analysis of glycan and RNA in single cells
title_fullStr scGR-seq: Integrated analysis of glycan and RNA in single cells
title_full_unstemmed scGR-seq: Integrated analysis of glycan and RNA in single cells
title_short scGR-seq: Integrated analysis of glycan and RNA in single cells
title_sort scgr-seq: integrated analysis of glycan and rna in single cells
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8861808/
https://www.ncbi.nlm.nih.gov/pubmed/35243371
http://dx.doi.org/10.1016/j.xpro.2022.101179
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