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Genome-scale modeling of yeast metabolism: retrospectives and perspectives
Yeasts have been widely used for production of bread, beer and wine, as well as for production of bioethanol, but they have also been designed as cell factories to produce various chemicals, advanced biofuels and recombinant proteins. To systematically understand and rationally engineer yeast metabo...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8862083/ https://www.ncbi.nlm.nih.gov/pubmed/35094064 http://dx.doi.org/10.1093/femsyr/foac003 |
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author | Chen, Yu Li, Feiran Nielsen, Jens |
author_facet | Chen, Yu Li, Feiran Nielsen, Jens |
author_sort | Chen, Yu |
collection | PubMed |
description | Yeasts have been widely used for production of bread, beer and wine, as well as for production of bioethanol, but they have also been designed as cell factories to produce various chemicals, advanced biofuels and recombinant proteins. To systematically understand and rationally engineer yeast metabolism, genome-scale metabolic models (GEMs) have been reconstructed for the model yeast Saccharomyces cerevisiae and nonconventional yeasts. Here, we review the historical development of yeast GEMs together with their recent applications, including metabolic flux prediction, cell factory design, culture condition optimization and multi-yeast comparative analysis. Furthermore, we present an emerging effort, namely the integration of proteome constraints into yeast GEMs, resulting in models with improved performance. At last, we discuss challenges and perspectives on the development of yeast GEMs and the integration of proteome constraints. |
format | Online Article Text |
id | pubmed-8862083 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-88620832022-02-23 Genome-scale modeling of yeast metabolism: retrospectives and perspectives Chen, Yu Li, Feiran Nielsen, Jens FEMS Yeast Res Minireview Yeasts have been widely used for production of bread, beer and wine, as well as for production of bioethanol, but they have also been designed as cell factories to produce various chemicals, advanced biofuels and recombinant proteins. To systematically understand and rationally engineer yeast metabolism, genome-scale metabolic models (GEMs) have been reconstructed for the model yeast Saccharomyces cerevisiae and nonconventional yeasts. Here, we review the historical development of yeast GEMs together with their recent applications, including metabolic flux prediction, cell factory design, culture condition optimization and multi-yeast comparative analysis. Furthermore, we present an emerging effort, namely the integration of proteome constraints into yeast GEMs, resulting in models with improved performance. At last, we discuss challenges and perspectives on the development of yeast GEMs and the integration of proteome constraints. Oxford University Press 2022-01-29 /pmc/articles/PMC8862083/ /pubmed/35094064 http://dx.doi.org/10.1093/femsyr/foac003 Text en © The Author(s) 2022. Published by Oxford University Press on behalf of FEMS. https://creativecommons.org/licenses/by-nc/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (https://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com |
spellingShingle | Minireview Chen, Yu Li, Feiran Nielsen, Jens Genome-scale modeling of yeast metabolism: retrospectives and perspectives |
title | Genome-scale modeling of yeast metabolism: retrospectives and perspectives |
title_full | Genome-scale modeling of yeast metabolism: retrospectives and perspectives |
title_fullStr | Genome-scale modeling of yeast metabolism: retrospectives and perspectives |
title_full_unstemmed | Genome-scale modeling of yeast metabolism: retrospectives and perspectives |
title_short | Genome-scale modeling of yeast metabolism: retrospectives and perspectives |
title_sort | genome-scale modeling of yeast metabolism: retrospectives and perspectives |
topic | Minireview |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8862083/ https://www.ncbi.nlm.nih.gov/pubmed/35094064 http://dx.doi.org/10.1093/femsyr/foac003 |
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