Cargando…

Biosynthesis of Silver Nanoparticles by Marine Actinobacterium Nocardiopsis dassonvillei and Exploring Their Therapeutic Potentials

Nanoparticles have recently emerged as a popular research topic. Because of their potential applications in therapeutic applications, biosynthesized silver nanoparticles (Bio-AgNPs) have gained much attention in recent years. Cell-free extracts (CFE) from a marine culture of actinobacteria and silve...

Descripción completa

Detalles Bibliográficos
Autores principales: Khalil, Maha A., El-Shanshoury, Abd El-Raheem R., Alghamdi, Maha A., Alsalmi, Fatin A., Mohamed, Samia F., Sun, Jianzhong, Ali, Sameh S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8862148/
https://www.ncbi.nlm.nih.gov/pubmed/35211096
http://dx.doi.org/10.3389/fmicb.2021.705673
_version_ 1784655003884978176
author Khalil, Maha A.
El-Shanshoury, Abd El-Raheem R.
Alghamdi, Maha A.
Alsalmi, Fatin A.
Mohamed, Samia F.
Sun, Jianzhong
Ali, Sameh S.
author_facet Khalil, Maha A.
El-Shanshoury, Abd El-Raheem R.
Alghamdi, Maha A.
Alsalmi, Fatin A.
Mohamed, Samia F.
Sun, Jianzhong
Ali, Sameh S.
author_sort Khalil, Maha A.
collection PubMed
description Nanoparticles have recently emerged as a popular research topic. Because of their potential applications in therapeutic applications, biosynthesized silver nanoparticles (Bio-AgNPs) have gained much attention in recent years. Cell-free extracts (CFE) from a marine culture of actinobacteria and silver nitrate were used to reduce Ag(+) ions and create Bio-AgNPs. Nocardiopsis dasonvillei KY772427, a new silver-tolerant actinomycete strain, was isolated from marine water and used to synthesize AgNPs. In order to characterize Bio-AgNPs, UV-Vis spectral analysis, Fourier transform infrared (FTIR), transmission electron microscopy (TEM), and dynamic light scattering spectroscopy (DLS) were all utilized. Using UV–Vis spectroscopy, a peak in the surface plasmon resonance (SPR) spectrum at 430 nm revealed the presence of Bio-AgNPs. The TEM revealed spherical AgNPs with a diameter of 29.28 nm. DLS determined that Bio-AgNPs have a diameter of 56.1 nm and a negative surface charge (−1.46 mV). The minimum inhibitory concentration (MIC) of Bio-AgNPs was determined against microbial strains. Using resazurin-based microtiter dilution, the synergistic effect of Bio-AgNPs with antimicrobials was investigated. Pseudomonas aeruginosa had the lowest MIC of Bio-AgNPs (4 μg/ml). Surprisingly, the combination of antimicrobials and Bio-AgNPs had a significant synergistic effect on the tested strains. The insecticidal activity of Bio-AgNPs (200 μg/ml) against Macrosiphum rosae was found to be maximal after 36 h. Additionally, Bio-AgNPs demonstrated significant scavenging activity against 2,2′-diphenyl-1-picrylhydrazyl (DPPH) and hydroxyl (OH(–)) radicals, with IC(50) values of 4.08 and 8.9 g/ml, respectively. In vitro studies using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay revealed a concentration-dependent decrease in cell viability when CaCo2 cells were exposed to Bio-AgNPs. With the decrease in cell viability, lactate dehydrogenase leakage (LDH) increased. The findings of this study open up a new avenue for the use of marine Nocardiopsis dasonvillei to produce Bio-AgNPs, which have significant antimicrobial, antioxidant, insecticidal, and anticancer potential.
format Online
Article
Text
id pubmed-8862148
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-88621482022-02-23 Biosynthesis of Silver Nanoparticles by Marine Actinobacterium Nocardiopsis dassonvillei and Exploring Their Therapeutic Potentials Khalil, Maha A. El-Shanshoury, Abd El-Raheem R. Alghamdi, Maha A. Alsalmi, Fatin A. Mohamed, Samia F. Sun, Jianzhong Ali, Sameh S. Front Microbiol Microbiology Nanoparticles have recently emerged as a popular research topic. Because of their potential applications in therapeutic applications, biosynthesized silver nanoparticles (Bio-AgNPs) have gained much attention in recent years. Cell-free extracts (CFE) from a marine culture of actinobacteria and silver nitrate were used to reduce Ag(+) ions and create Bio-AgNPs. Nocardiopsis dasonvillei KY772427, a new silver-tolerant actinomycete strain, was isolated from marine water and used to synthesize AgNPs. In order to characterize Bio-AgNPs, UV-Vis spectral analysis, Fourier transform infrared (FTIR), transmission electron microscopy (TEM), and dynamic light scattering spectroscopy (DLS) were all utilized. Using UV–Vis spectroscopy, a peak in the surface plasmon resonance (SPR) spectrum at 430 nm revealed the presence of Bio-AgNPs. The TEM revealed spherical AgNPs with a diameter of 29.28 nm. DLS determined that Bio-AgNPs have a diameter of 56.1 nm and a negative surface charge (−1.46 mV). The minimum inhibitory concentration (MIC) of Bio-AgNPs was determined against microbial strains. Using resazurin-based microtiter dilution, the synergistic effect of Bio-AgNPs with antimicrobials was investigated. Pseudomonas aeruginosa had the lowest MIC of Bio-AgNPs (4 μg/ml). Surprisingly, the combination of antimicrobials and Bio-AgNPs had a significant synergistic effect on the tested strains. The insecticidal activity of Bio-AgNPs (200 μg/ml) against Macrosiphum rosae was found to be maximal after 36 h. Additionally, Bio-AgNPs demonstrated significant scavenging activity against 2,2′-diphenyl-1-picrylhydrazyl (DPPH) and hydroxyl (OH(–)) radicals, with IC(50) values of 4.08 and 8.9 g/ml, respectively. In vitro studies using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay revealed a concentration-dependent decrease in cell viability when CaCo2 cells were exposed to Bio-AgNPs. With the decrease in cell viability, lactate dehydrogenase leakage (LDH) increased. The findings of this study open up a new avenue for the use of marine Nocardiopsis dasonvillei to produce Bio-AgNPs, which have significant antimicrobial, antioxidant, insecticidal, and anticancer potential. Frontiers Media S.A. 2022-02-03 /pmc/articles/PMC8862148/ /pubmed/35211096 http://dx.doi.org/10.3389/fmicb.2021.705673 Text en Copyright © 2022 Khalil, El-Shanshoury, Alghamdi, Alsalmi, Mohamed, Sun and Ali. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Khalil, Maha A.
El-Shanshoury, Abd El-Raheem R.
Alghamdi, Maha A.
Alsalmi, Fatin A.
Mohamed, Samia F.
Sun, Jianzhong
Ali, Sameh S.
Biosynthesis of Silver Nanoparticles by Marine Actinobacterium Nocardiopsis dassonvillei and Exploring Their Therapeutic Potentials
title Biosynthesis of Silver Nanoparticles by Marine Actinobacterium Nocardiopsis dassonvillei and Exploring Their Therapeutic Potentials
title_full Biosynthesis of Silver Nanoparticles by Marine Actinobacterium Nocardiopsis dassonvillei and Exploring Their Therapeutic Potentials
title_fullStr Biosynthesis of Silver Nanoparticles by Marine Actinobacterium Nocardiopsis dassonvillei and Exploring Their Therapeutic Potentials
title_full_unstemmed Biosynthesis of Silver Nanoparticles by Marine Actinobacterium Nocardiopsis dassonvillei and Exploring Their Therapeutic Potentials
title_short Biosynthesis of Silver Nanoparticles by Marine Actinobacterium Nocardiopsis dassonvillei and Exploring Their Therapeutic Potentials
title_sort biosynthesis of silver nanoparticles by marine actinobacterium nocardiopsis dassonvillei and exploring their therapeutic potentials
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8862148/
https://www.ncbi.nlm.nih.gov/pubmed/35211096
http://dx.doi.org/10.3389/fmicb.2021.705673
work_keys_str_mv AT khalilmahaa biosynthesisofsilvernanoparticlesbymarineactinobacteriumnocardiopsisdassonvilleiandexploringtheirtherapeuticpotentials
AT elshanshouryabdelraheemr biosynthesisofsilvernanoparticlesbymarineactinobacteriumnocardiopsisdassonvilleiandexploringtheirtherapeuticpotentials
AT alghamdimahaa biosynthesisofsilvernanoparticlesbymarineactinobacteriumnocardiopsisdassonvilleiandexploringtheirtherapeuticpotentials
AT alsalmifatina biosynthesisofsilvernanoparticlesbymarineactinobacteriumnocardiopsisdassonvilleiandexploringtheirtherapeuticpotentials
AT mohamedsamiaf biosynthesisofsilvernanoparticlesbymarineactinobacteriumnocardiopsisdassonvilleiandexploringtheirtherapeuticpotentials
AT sunjianzhong biosynthesisofsilvernanoparticlesbymarineactinobacteriumnocardiopsisdassonvilleiandexploringtheirtherapeuticpotentials
AT alisamehs biosynthesisofsilvernanoparticlesbymarineactinobacteriumnocardiopsisdassonvilleiandexploringtheirtherapeuticpotentials