Rapid directed molecular evolution of fluorescent proteins in mammalian cells

In vivo imaging of model organisms is heavily reliant on fluorescent proteins with high intracellular brightness. Here we describe a practical method for rapid optimization of fluorescent proteins via directed molecular evolution in cultured mammalian cells. Using this method, we were able to perfor...

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Detalles Bibliográficos
Autores principales: Babakhanova, Siranush, Jung, Erica E., Namikawa, Kazuhiko, Zhang, Hanbin, Wang, Yangdong, Subach, Oksana M., Korzhenevskiy, Dmitry A., Rakitina, Tatiana V., Xiao, Xian, Wang, Wenjing, Shi, Jing, Drobizhev, Mikhail, Park, Demian, Eisenhard, Lea, Tang, Hongyun, Köster, Reinhard W., Subach, Fedor V., Boyden, Edward S., Piatkevich, Kiryl D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley & Sons, Inc. 2021
Materias:
Methods and Applications
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8862398/
https://www.ncbi.nlm.nih.gov/pubmed/34913537
http://dx.doi.org/10.1002/pro.4261

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