Misidentification of the SARS-CoV-2 Mu variant using commercial mutation screening assays

Detection of mutations by multiplex real-time RT-PCR is a widely used method for the screening of SARS-CoV-2 variants, but this method has several limitations. We describe three cases in which a Mu strain containing the mutation K417N was initially misclassified as the Beta variant. We recommend the...

Descripción completa

Detalles Bibliográficos
Autores principales: Alejo-Cancho, Izaskun, Gual-de-Torrella, Ana, Gallego, Mikel, Urrutikoetxea-Gutierrez, Mikel, Lejarraga, Clara, López de Goikoetxea, Maria José
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Vienna 2022
Materias:
Brief Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8863091/
https://www.ncbi.nlm.nih.gov/pubmed/35194675
http://dx.doi.org/10.1007/s00705-022-05395-w
_version_ 1784655166972100608
author Alejo-Cancho, Izaskun
Gual-de-Torrella, Ana
Gallego, Mikel
Urrutikoetxea-Gutierrez, Mikel
Lejarraga, Clara
López de Goikoetxea, Maria José
author_facet Alejo-Cancho, Izaskun
Gual-de-Torrella, Ana
Gallego, Mikel
Urrutikoetxea-Gutierrez, Mikel
Lejarraga, Clara
López de Goikoetxea, Maria José
author_sort Alejo-Cancho, Izaskun
collection PubMed
description Detection of mutations by multiplex real-time RT-PCR is a widely used method for the screening of SARS-CoV-2 variants, but this method has several limitations. We describe three cases in which a Mu strain containing the mutation K417N was initially misclassified as the Beta variant. We recommend the detection of P681H to distinguish between these two variants. Our experience highlights the importance of keeping track of new variants and mutations in order to adapt the current workflows.
format Online
Article
Text
id pubmed-8863091
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Springer Vienna
record_format MEDLINE/PubMed
spelling pubmed-88630912022-02-23 Misidentification of the SARS-CoV-2 Mu variant using commercial mutation screening assays Alejo-Cancho, Izaskun Gual-de-Torrella, Ana Gallego, Mikel Urrutikoetxea-Gutierrez, Mikel Lejarraga, Clara López de Goikoetxea, Maria José Arch Virol Brief Report Detection of mutations by multiplex real-time RT-PCR is a widely used method for the screening of SARS-CoV-2 variants, but this method has several limitations. We describe three cases in which a Mu strain containing the mutation K417N was initially misclassified as the Beta variant. We recommend the detection of P681H to distinguish between these two variants. Our experience highlights the importance of keeping track of new variants and mutations in order to adapt the current workflows. Springer Vienna 2022-02-22 2022 /pmc/articles/PMC8863091/ /pubmed/35194675 http://dx.doi.org/10.1007/s00705-022-05395-w Text en © The Author(s), under exclusive licence to Springer-Verlag GmbH Austria, part of Springer Nature 2022 This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic.
spellingShingle Brief Report
Alejo-Cancho, Izaskun
Gual-de-Torrella, Ana
Gallego, Mikel
Urrutikoetxea-Gutierrez, Mikel
Lejarraga, Clara
López de Goikoetxea, Maria José
Misidentification of the SARS-CoV-2 Mu variant using commercial mutation screening assays
title Misidentification of the SARS-CoV-2 Mu variant using commercial mutation screening assays
title_full Misidentification of the SARS-CoV-2 Mu variant using commercial mutation screening assays
title_fullStr Misidentification of the SARS-CoV-2 Mu variant using commercial mutation screening assays
title_full_unstemmed Misidentification of the SARS-CoV-2 Mu variant using commercial mutation screening assays
title_short Misidentification of the SARS-CoV-2 Mu variant using commercial mutation screening assays
title_sort misidentification of the sars-cov-2 mu variant using commercial mutation screening assays
topic Brief Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8863091/
https://www.ncbi.nlm.nih.gov/pubmed/35194675
http://dx.doi.org/10.1007/s00705-022-05395-w
work_keys_str_mv AT alejocanchoizaskun misidentificationofthesarscov2muvariantusingcommercialmutationscreeningassays
AT gualdetorrellaana misidentificationofthesarscov2muvariantusingcommercialmutationscreeningassays
AT gallegomikel misidentificationofthesarscov2muvariantusingcommercialmutationscreeningassays
AT urrutikoetxeagutierrezmikel misidentificationofthesarscov2muvariantusingcommercialmutationscreeningassays
AT lejarragaclara misidentificationofthesarscov2muvariantusingcommercialmutationscreeningassays
AT lopezdegoikoetxeamariajose misidentificationofthesarscov2muvariantusingcommercialmutationscreeningassays

Ejemplares similares