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SHMT2 Drives the Progression of Colorectal Cancer by Regulating UHRF1 Expression
INTRODUCTION: Serine hydroxymethyltransferase 2 (SHMT2) has a critical role in serine-glycine metabolism to drive cancer cell proliferation. Yet, the function of SHMT2 in tumorigenesis, especially in human colorectal cancer (CRC) progression, remains largely unclear. MATERIALS AND METHODS: CRC and p...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8863481/ https://www.ncbi.nlm.nih.gov/pubmed/35211429 http://dx.doi.org/10.1155/2022/3758697 |
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author | Cui, Ximao Cui, Yanfen Du, Tao Jiang, Xiaohua Song, Chun Zhang, Shun Ma, Chiye Liu, Yun Ni, Qing Gao, Yuzhe Wang, Guanghui |
author_facet | Cui, Ximao Cui, Yanfen Du, Tao Jiang, Xiaohua Song, Chun Zhang, Shun Ma, Chiye Liu, Yun Ni, Qing Gao, Yuzhe Wang, Guanghui |
author_sort | Cui, Ximao |
collection | PubMed |
description | INTRODUCTION: Serine hydroxymethyltransferase 2 (SHMT2) has a critical role in serine-glycine metabolism to drive cancer cell proliferation. Yet, the function of SHMT2 in tumorigenesis, especially in human colorectal cancer (CRC) progression, remains largely unclear. MATERIALS AND METHODS: CRC and paired normal samples were collected in the Department of Colorectal Surgery, Xinhua Hospital, Shanghai Jiao Tong University School of Medicine, and assessed by real-time polymerase chain reaction (qPCR) analysis, western blot (WB), and immunohistochemistry (IHC). Moreover, SHMT2 expression in human CRC cells was identified by qPCR and WB. The CRC cell proliferation, migration, and invasion after SHMT2 knockdown were explored through in vitro and in vivo assays. mRNA-seq assays were used to investigate the underlying mechanisms behind the SHMT2 function. RESULTS: It was found that SHMT2 mRNA and protein were overexpressed in CRC tissue compared to the levels in normal mucosa. Positive expression of SHMT2 was significantly correlated with TNM stage and lymph node metastasis, and elevated expression of SHMT2 resulted as an independent prognostic factor in patients with CRC. SHMT2 knockdown impaired the proliferation of CRC in vitro and in vivo and induced cell cycle arrest by regulating UHRF1 expression. CONCLUSION: Taken together, our findings reveal that UHRF1 is a novel target gene of SHMT2, which can be used as a potential therapeutic strategy for CRC therapy. |
format | Online Article Text |
id | pubmed-8863481 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Hindawi |
record_format | MEDLINE/PubMed |
spelling | pubmed-88634812022-02-23 SHMT2 Drives the Progression of Colorectal Cancer by Regulating UHRF1 Expression Cui, Ximao Cui, Yanfen Du, Tao Jiang, Xiaohua Song, Chun Zhang, Shun Ma, Chiye Liu, Yun Ni, Qing Gao, Yuzhe Wang, Guanghui Can J Gastroenterol Hepatol Research Article INTRODUCTION: Serine hydroxymethyltransferase 2 (SHMT2) has a critical role in serine-glycine metabolism to drive cancer cell proliferation. Yet, the function of SHMT2 in tumorigenesis, especially in human colorectal cancer (CRC) progression, remains largely unclear. MATERIALS AND METHODS: CRC and paired normal samples were collected in the Department of Colorectal Surgery, Xinhua Hospital, Shanghai Jiao Tong University School of Medicine, and assessed by real-time polymerase chain reaction (qPCR) analysis, western blot (WB), and immunohistochemistry (IHC). Moreover, SHMT2 expression in human CRC cells was identified by qPCR and WB. The CRC cell proliferation, migration, and invasion after SHMT2 knockdown were explored through in vitro and in vivo assays. mRNA-seq assays were used to investigate the underlying mechanisms behind the SHMT2 function. RESULTS: It was found that SHMT2 mRNA and protein were overexpressed in CRC tissue compared to the levels in normal mucosa. Positive expression of SHMT2 was significantly correlated with TNM stage and lymph node metastasis, and elevated expression of SHMT2 resulted as an independent prognostic factor in patients with CRC. SHMT2 knockdown impaired the proliferation of CRC in vitro and in vivo and induced cell cycle arrest by regulating UHRF1 expression. CONCLUSION: Taken together, our findings reveal that UHRF1 is a novel target gene of SHMT2, which can be used as a potential therapeutic strategy for CRC therapy. Hindawi 2022-02-15 /pmc/articles/PMC8863481/ /pubmed/35211429 http://dx.doi.org/10.1155/2022/3758697 Text en Copyright © 2022 Ximao Cui et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Cui, Ximao Cui, Yanfen Du, Tao Jiang, Xiaohua Song, Chun Zhang, Shun Ma, Chiye Liu, Yun Ni, Qing Gao, Yuzhe Wang, Guanghui SHMT2 Drives the Progression of Colorectal Cancer by Regulating UHRF1 Expression |
title | SHMT2 Drives the Progression of Colorectal Cancer by Regulating UHRF1 Expression |
title_full | SHMT2 Drives the Progression of Colorectal Cancer by Regulating UHRF1 Expression |
title_fullStr | SHMT2 Drives the Progression of Colorectal Cancer by Regulating UHRF1 Expression |
title_full_unstemmed | SHMT2 Drives the Progression of Colorectal Cancer by Regulating UHRF1 Expression |
title_short | SHMT2 Drives the Progression of Colorectal Cancer by Regulating UHRF1 Expression |
title_sort | shmt2 drives the progression of colorectal cancer by regulating uhrf1 expression |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8863481/ https://www.ncbi.nlm.nih.gov/pubmed/35211429 http://dx.doi.org/10.1155/2022/3758697 |
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