A multiparametric fluorescence assay for screening aptamer–protein interactions based on microbeads

For improving aptamer-ligand binding we have developed a screening system that defines optimal binding buffer composition. Using multiplex assays, one buffer system is needed which guarantees the specific binding of all aptamers. We investigated nine peer-reviewed DNA aptamers. Non-specific binding...

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Detalles Bibliográficos
Autores principales: Schmidt, Carsten, Kammel, Anne, Tanner, Julian A., Kinghorn, Andrew B., Khan, Muhammad Moman, Lehmann, Werner, Menger, Marcus, Schedler, Uwe, Schierack, Peter, Rödiger, Stefan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8863788/
https://www.ncbi.nlm.nih.gov/pubmed/35194086
http://dx.doi.org/10.1038/s41598-022-06817-0

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