Screening methods for enzyme-mediated alcohol oxidation

Alcohol oxidation for the generation of carbonyl groups, is an essential reaction for the preparation of fine chemicals. Although a number of chemical procedures have been reported, biocatalysis is a promising alternative for more sustainable and selective processes. To speed up the discovery of nov...

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Autores principales: Contente, Martina L., Marzuoli, Irene, Iding, Hans, Wetzl, Dennis, Puentener, Kurt, Hanlon, Steven P., Paradisi, Francesca
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8864024/
https://www.ncbi.nlm.nih.gov/pubmed/35194101
http://dx.doi.org/10.1038/s41598-022-07008-7
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author Contente, Martina L.
Marzuoli, Irene
Iding, Hans
Wetzl, Dennis
Puentener, Kurt
Hanlon, Steven P.
Paradisi, Francesca
author_facet Contente, Martina L.
Marzuoli, Irene
Iding, Hans
Wetzl, Dennis
Puentener, Kurt
Hanlon, Steven P.
Paradisi, Francesca
author_sort Contente, Martina L.
collection PubMed
description Alcohol oxidation for the generation of carbonyl groups, is an essential reaction for the preparation of fine chemicals. Although a number of chemical procedures have been reported, biocatalysis is a promising alternative for more sustainable and selective processes. To speed up the discovery of novel (bio)catalysts for industrial applications, efficient screening approaches need to be established. Here, we report on an enzyme-mediated alcohol oxidation screening platform to rapidly detect the activities and selectivities of three classes of biocatalysts; ketoreductases (KREDs), alcohol oxidases (AlcOXs) and laccase-mediator systems (LMSs) with diverse substrates.
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spelling pubmed-88640242022-02-23 Screening methods for enzyme-mediated alcohol oxidation Contente, Martina L. Marzuoli, Irene Iding, Hans Wetzl, Dennis Puentener, Kurt Hanlon, Steven P. Paradisi, Francesca Sci Rep Article Alcohol oxidation for the generation of carbonyl groups, is an essential reaction for the preparation of fine chemicals. Although a number of chemical procedures have been reported, biocatalysis is a promising alternative for more sustainable and selective processes. To speed up the discovery of novel (bio)catalysts for industrial applications, efficient screening approaches need to be established. Here, we report on an enzyme-mediated alcohol oxidation screening platform to rapidly detect the activities and selectivities of three classes of biocatalysts; ketoreductases (KREDs), alcohol oxidases (AlcOXs) and laccase-mediator systems (LMSs) with diverse substrates. Nature Publishing Group UK 2022-02-22 /pmc/articles/PMC8864024/ /pubmed/35194101 http://dx.doi.org/10.1038/s41598-022-07008-7 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Contente, Martina L.
Marzuoli, Irene
Iding, Hans
Wetzl, Dennis
Puentener, Kurt
Hanlon, Steven P.
Paradisi, Francesca
Screening methods for enzyme-mediated alcohol oxidation
title Screening methods for enzyme-mediated alcohol oxidation
title_full Screening methods for enzyme-mediated alcohol oxidation
title_fullStr Screening methods for enzyme-mediated alcohol oxidation
title_full_unstemmed Screening methods for enzyme-mediated alcohol oxidation
title_short Screening methods for enzyme-mediated alcohol oxidation
title_sort screening methods for enzyme-mediated alcohol oxidation
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8864024/
https://www.ncbi.nlm.nih.gov/pubmed/35194101
http://dx.doi.org/10.1038/s41598-022-07008-7
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