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moc-6/MOCS2A is necessary for molybdenum cofactor synthesis in C. elegans

Molybdenum cofactor (Moco) is an essential prosthetic group that mediates the activity of 4 animal oxidases and is required for viability. Humans with mutations in the genes encoding Moco-biosynthetic enzymes suffer from Moco deficiency, a neonatal lethal inborn error of metabolism. Caenorhabditis e...

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Autores principales: Snoozy, Jennifer, Breen, Peter C, Ruvkun, Gary, Warnhoff, Kurt
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Caltech Library 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8864482/
https://www.ncbi.nlm.nih.gov/pubmed/35224462
http://dx.doi.org/10.17912/micropub.biology.000531
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author Snoozy, Jennifer
Breen, Peter C
Ruvkun, Gary
Warnhoff, Kurt
author_facet Snoozy, Jennifer
Breen, Peter C
Ruvkun, Gary
Warnhoff, Kurt
author_sort Snoozy, Jennifer
collection PubMed
description Molybdenum cofactor (Moco) is an essential prosthetic group that mediates the activity of 4 animal oxidases and is required for viability. Humans with mutations in the genes encoding Moco-biosynthetic enzymes suffer from Moco deficiency, a neonatal lethal inborn error of metabolism. Caenorhabditis elegans has recently emerged as a useful and tractable genetic discovery engine for Moco biology. Here, we identify and characterize K10D2.7/moc-6, the C. elegans ortholog of human MOCS2A, a sulfur-carrier protein essential for Moco synthesis. Using CRISPR/Cas9 gene editing, we generate 3 null mutations in K10D2.7/moc-6 and with these alleles genetically demonstrate that K10D2.7/moc-6 is necessary for endogenous Moco synthesis in C. elegans.
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spelling pubmed-88644822022-02-24 moc-6/MOCS2A is necessary for molybdenum cofactor synthesis in C. elegans Snoozy, Jennifer Breen, Peter C Ruvkun, Gary Warnhoff, Kurt MicroPubl Biol Materials and Reagents Molybdenum cofactor (Moco) is an essential prosthetic group that mediates the activity of 4 animal oxidases and is required for viability. Humans with mutations in the genes encoding Moco-biosynthetic enzymes suffer from Moco deficiency, a neonatal lethal inborn error of metabolism. Caenorhabditis elegans has recently emerged as a useful and tractable genetic discovery engine for Moco biology. Here, we identify and characterize K10D2.7/moc-6, the C. elegans ortholog of human MOCS2A, a sulfur-carrier protein essential for Moco synthesis. Using CRISPR/Cas9 gene editing, we generate 3 null mutations in K10D2.7/moc-6 and with these alleles genetically demonstrate that K10D2.7/moc-6 is necessary for endogenous Moco synthesis in C. elegans. Caltech Library 2022-02-22 /pmc/articles/PMC8864482/ /pubmed/35224462 http://dx.doi.org/10.17912/micropub.biology.000531 Text en Copyright: © 2022 by the authors https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Materials and Reagents
Snoozy, Jennifer
Breen, Peter C
Ruvkun, Gary
Warnhoff, Kurt
moc-6/MOCS2A is necessary for molybdenum cofactor synthesis in C. elegans
title moc-6/MOCS2A is necessary for molybdenum cofactor synthesis in C. elegans
title_full moc-6/MOCS2A is necessary for molybdenum cofactor synthesis in C. elegans
title_fullStr moc-6/MOCS2A is necessary for molybdenum cofactor synthesis in C. elegans
title_full_unstemmed moc-6/MOCS2A is necessary for molybdenum cofactor synthesis in C. elegans
title_short moc-6/MOCS2A is necessary for molybdenum cofactor synthesis in C. elegans
title_sort moc-6/mocs2a is necessary for molybdenum cofactor synthesis in c. elegans
topic Materials and Reagents
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8864482/
https://www.ncbi.nlm.nih.gov/pubmed/35224462
http://dx.doi.org/10.17912/micropub.biology.000531
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