mNG-tagged mls-2 knock-in alleles in C. elegans

The Caenorhabditis elegans HMX/NKX MLS-2 transcription factor was previously shown to play sequential roles in AWC general identity and the stochastic AWC(ON)/AWC(OFF) subtype choice during embryogenesis. Here we analyze the expression pattern of endogenous mls-2 during AWC development using mNeonGr...

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Detalles Bibliográficos
Autores principales: Xiong, Rui, Hsieh, Yi-Wen, Chuang, Chiou-Fen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Caltech Library 2022
Materias:
Findings Previously Not Shown
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8864483/
https://www.ncbi.nlm.nih.gov/pubmed/35224463
http://dx.doi.org/10.17912/micropub.biology.000529
Descripción
Sumario:The Caenorhabditis elegans HMX/NKX MLS-2 transcription factor was previously shown to play sequential roles in AWC general identity and the stochastic AWC(ON)/AWC(OFF) subtype choice during embryogenesis. Here we analyze the expression pattern of endogenous mls-2 during AWC development using mNeonGreen (mNG) knock-in strains. Similar to transgenic GFP::MLS-2, functional mNG::MLS-2 knock-in displayed nuclear localization in AWC precursor cells but was not observed in AWC during the later embryonic stage. These results suggest that the expression of mls-2 is below the detectable level and/or the stability of MLS-2 protein is very low in AWC cells.

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