Cargando…
Vimentin Intermediate Filaments Mediate Cell Morphology on Viscoelastic Substrates
[Image: see text] The ability of cells to take and change shape is a fundamental feature underlying development, wound repair, and tissue maintenance. Central to this process is physical and signaling interactions between the three cytoskeletal polymeric networks: F-actin, microtubules, and intermed...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Chemical Society
2022
|
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8864613/ https://www.ncbi.nlm.nih.gov/pubmed/34995457 http://dx.doi.org/10.1021/acsabm.1c01046 |
_version_ | 1784655495090405376 |
---|---|
author | Swoger, Maxx Gupta, Sarthak Charrier, Elisabeth E. Bates, Michael Hehnly, Heidi Patteson, Alison E. |
author_facet | Swoger, Maxx Gupta, Sarthak Charrier, Elisabeth E. Bates, Michael Hehnly, Heidi Patteson, Alison E. |
author_sort | Swoger, Maxx |
collection | PubMed |
description | [Image: see text] The ability of cells to take and change shape is a fundamental feature underlying development, wound repair, and tissue maintenance. Central to this process is physical and signaling interactions between the three cytoskeletal polymeric networks: F-actin, microtubules, and intermediate filaments (IFs). Vimentin is an IF protein that is essential to the mechanical resilience of cells and regulates cross-talk among the cytoskeleton, but its role in how cells sense and respond to the surrounding extracellular matrix is largely unclear. To investigate vimentin’s role in substrate sensing, we designed polyacrylamide hydrogels that mimic the elastic and viscoelastic nature of in vivo tissues. Using wild-type and vimentin-null mouse embryonic fibroblasts, we show that vimentin enhances cell spreading on viscoelastic substrates, even though it has little effect in the limit of purely elastic substrates. Our results provide compelling evidence that vimentin modulates how cells sense and respond to their environment and thus plays a key role in cell mechanosensing. |
format | Online Article Text |
id | pubmed-8864613 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | American Chemical Society |
record_format | MEDLINE/PubMed |
spelling | pubmed-88646132022-02-23 Vimentin Intermediate Filaments Mediate Cell Morphology on Viscoelastic Substrates Swoger, Maxx Gupta, Sarthak Charrier, Elisabeth E. Bates, Michael Hehnly, Heidi Patteson, Alison E. ACS Appl Bio Mater [Image: see text] The ability of cells to take and change shape is a fundamental feature underlying development, wound repair, and tissue maintenance. Central to this process is physical and signaling interactions between the three cytoskeletal polymeric networks: F-actin, microtubules, and intermediate filaments (IFs). Vimentin is an IF protein that is essential to the mechanical resilience of cells and regulates cross-talk among the cytoskeleton, but its role in how cells sense and respond to the surrounding extracellular matrix is largely unclear. To investigate vimentin’s role in substrate sensing, we designed polyacrylamide hydrogels that mimic the elastic and viscoelastic nature of in vivo tissues. Using wild-type and vimentin-null mouse embryonic fibroblasts, we show that vimentin enhances cell spreading on viscoelastic substrates, even though it has little effect in the limit of purely elastic substrates. Our results provide compelling evidence that vimentin modulates how cells sense and respond to their environment and thus plays a key role in cell mechanosensing. American Chemical Society 2022-01-07 2022-02-21 /pmc/articles/PMC8864613/ /pubmed/34995457 http://dx.doi.org/10.1021/acsabm.1c01046 Text en © 2022 The Authors. Published by American Chemical Society https://creativecommons.org/licenses/by/4.0/Permits the broadest form of re-use including for commercial purposes, provided that author attribution and integrity are maintained (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Swoger, Maxx Gupta, Sarthak Charrier, Elisabeth E. Bates, Michael Hehnly, Heidi Patteson, Alison E. Vimentin Intermediate Filaments Mediate Cell Morphology on Viscoelastic Substrates |
title | Vimentin Intermediate Filaments Mediate Cell Morphology
on Viscoelastic Substrates |
title_full | Vimentin Intermediate Filaments Mediate Cell Morphology
on Viscoelastic Substrates |
title_fullStr | Vimentin Intermediate Filaments Mediate Cell Morphology
on Viscoelastic Substrates |
title_full_unstemmed | Vimentin Intermediate Filaments Mediate Cell Morphology
on Viscoelastic Substrates |
title_short | Vimentin Intermediate Filaments Mediate Cell Morphology
on Viscoelastic Substrates |
title_sort | vimentin intermediate filaments mediate cell morphology
on viscoelastic substrates |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8864613/ https://www.ncbi.nlm.nih.gov/pubmed/34995457 http://dx.doi.org/10.1021/acsabm.1c01046 |
work_keys_str_mv | AT swogermaxx vimentinintermediatefilamentsmediatecellmorphologyonviscoelasticsubstrates AT guptasarthak vimentinintermediatefilamentsmediatecellmorphologyonviscoelasticsubstrates AT charrierelisabethe vimentinintermediatefilamentsmediatecellmorphologyonviscoelasticsubstrates AT batesmichael vimentinintermediatefilamentsmediatecellmorphologyonviscoelasticsubstrates AT hehnlyheidi vimentinintermediatefilamentsmediatecellmorphologyonviscoelasticsubstrates AT pattesonalisone vimentinintermediatefilamentsmediatecellmorphologyonviscoelasticsubstrates |