Physical activity specifically evokes release of cell-free DNA from granulocytes thereby affecting liquid biopsy

Physical activity impacts immune homeostasis and leads to rapid and marked increase in cell-free DNA (cfDNA). However, the origin of cfDNA during exercise remains elusive and it is unknown if physical activity could improve or interfere with methylation based liquid biopsy. We analyzed the methylati...

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Autores principales: Neuberger, Elmo W. I., Sontag, Stephanie, Brahmer, Alexandra, Philippi, Keito F. A., Radsak, Markus P., Wagner, Wolfgang, Simon, Perikles
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2022
Materias:
Short Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8864902/
https://www.ncbi.nlm.nih.gov/pubmed/35193681
http://dx.doi.org/10.1186/s13148-022-01245-3
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author Neuberger, Elmo W. I.
Sontag, Stephanie
Brahmer, Alexandra
Philippi, Keito F. A.
Radsak, Markus P.
Wagner, Wolfgang
Simon, Perikles
author_facet Neuberger, Elmo W. I.
Sontag, Stephanie
Brahmer, Alexandra
Philippi, Keito F. A.
Radsak, Markus P.
Wagner, Wolfgang
Simon, Perikles
author_sort Neuberger, Elmo W. I.
collection PubMed
description Physical activity impacts immune homeostasis and leads to rapid and marked increase in cell-free DNA (cfDNA). However, the origin of cfDNA during exercise remains elusive and it is unknown if physical activity could improve or interfere with methylation based liquid biopsy. We analyzed the methylation levels of four validated CpGs representing cfDNA from granulocytes, lymphocytes, monocytes, and non-hematopoietic cells, in healthy individuals in response to exercise, and in patients with hematological malignancies under resting conditions. The analysis revealed that physical activity almost exclusively triggered DNA release from granulocytes, highlighting the relevance as a pre-analytical variable which could compromise diagnostic accuracy. GRAPHICAL ABSTRACT: [Image: see text] SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s13148-022-01245-3.
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spelling pubmed-88649022022-02-28 Physical activity specifically evokes release of cell-free DNA from granulocytes thereby affecting liquid biopsy Neuberger, Elmo W. I. Sontag, Stephanie Brahmer, Alexandra Philippi, Keito F. A. Radsak, Markus P. Wagner, Wolfgang Simon, Perikles Clin Epigenetics Short Report Physical activity impacts immune homeostasis and leads to rapid and marked increase in cell-free DNA (cfDNA). However, the origin of cfDNA during exercise remains elusive and it is unknown if physical activity could improve or interfere with methylation based liquid biopsy. We analyzed the methylation levels of four validated CpGs representing cfDNA from granulocytes, lymphocytes, monocytes, and non-hematopoietic cells, in healthy individuals in response to exercise, and in patients with hematological malignancies under resting conditions. The analysis revealed that physical activity almost exclusively triggered DNA release from granulocytes, highlighting the relevance as a pre-analytical variable which could compromise diagnostic accuracy. GRAPHICAL ABSTRACT: [Image: see text] SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s13148-022-01245-3. BioMed Central 2022-02-22 /pmc/articles/PMC8864902/ /pubmed/35193681 http://dx.doi.org/10.1186/s13148-022-01245-3 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Short Report
Neuberger, Elmo W. I.
Sontag, Stephanie
Brahmer, Alexandra
Philippi, Keito F. A.
Radsak, Markus P.
Wagner, Wolfgang
Simon, Perikles
Physical activity specifically evokes release of cell-free DNA from granulocytes thereby affecting liquid biopsy
title Physical activity specifically evokes release of cell-free DNA from granulocytes thereby affecting liquid biopsy
title_full Physical activity specifically evokes release of cell-free DNA from granulocytes thereby affecting liquid biopsy
title_fullStr Physical activity specifically evokes release of cell-free DNA from granulocytes thereby affecting liquid biopsy
title_full_unstemmed Physical activity specifically evokes release of cell-free DNA from granulocytes thereby affecting liquid biopsy
title_short Physical activity specifically evokes release of cell-free DNA from granulocytes thereby affecting liquid biopsy
title_sort physical activity specifically evokes release of cell-free dna from granulocytes thereby affecting liquid biopsy
topic Short Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8864902/
https://www.ncbi.nlm.nih.gov/pubmed/35193681
http://dx.doi.org/10.1186/s13148-022-01245-3
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