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Metabolic engineering of Escherichia coli for efficient biosynthesis of butyl acetate
BACKGROUND: Butyl acetate is a versatile compound that is widely used in the chemical and food industry. The conventional butyl acetate synthesis via Fischer esterification of butanol and acetic acid using catalytic strong acids under high temperature is not environmentally benign. Alternative lipas...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8864926/ https://www.ncbi.nlm.nih.gov/pubmed/35193559 http://dx.doi.org/10.1186/s12934-022-01755-y |
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author | Ku, Jason T. Chen, Arvin Y. Lan, Ethan I. |
author_facet | Ku, Jason T. Chen, Arvin Y. Lan, Ethan I. |
author_sort | Ku, Jason T. |
collection | PubMed |
description | BACKGROUND: Butyl acetate is a versatile compound that is widely used in the chemical and food industry. The conventional butyl acetate synthesis via Fischer esterification of butanol and acetic acid using catalytic strong acids under high temperature is not environmentally benign. Alternative lipase-catalyzed ester formation requires a significant amount of organic solvent which also presents another environmental challenge. Therefore, a microbial cell factory capable of producing butyl acetate through fermentation of renewable resources would provide a greener approach to butyl acetate production. RESULT: Here, we developed a metabolically engineered strain of Escherichia coli that efficiently converts glucose to butyl acetate. A modified Clostridium CoA-dependent butanol production pathway was used to synthesize butanol which was then condensed with acetyl-CoA through an alcohol acetyltransferase. Optimization of alcohol acetyltransferase expression and redox balance with auto-inducible fermentative controlled gene expression led to an effective titer of 22.8 ± 1.8 g/L butyl acetate produced in a bench-top bioreactor. CONCLUSION: Building on the well-developed Clostridium CoA-dependent butanol biosynthetic pathway, expression of an alcohol acetyltransferase converts the butanol produced into butyl acetate. The results from this study provided a strain of E. coli capable of directly producing butyl acetate from renewable resources at ambient conditions. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12934-022-01755-y. |
format | Online Article Text |
id | pubmed-8864926 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-88649262022-02-28 Metabolic engineering of Escherichia coli for efficient biosynthesis of butyl acetate Ku, Jason T. Chen, Arvin Y. Lan, Ethan I. Microb Cell Fact Research BACKGROUND: Butyl acetate is a versatile compound that is widely used in the chemical and food industry. The conventional butyl acetate synthesis via Fischer esterification of butanol and acetic acid using catalytic strong acids under high temperature is not environmentally benign. Alternative lipase-catalyzed ester formation requires a significant amount of organic solvent which also presents another environmental challenge. Therefore, a microbial cell factory capable of producing butyl acetate through fermentation of renewable resources would provide a greener approach to butyl acetate production. RESULT: Here, we developed a metabolically engineered strain of Escherichia coli that efficiently converts glucose to butyl acetate. A modified Clostridium CoA-dependent butanol production pathway was used to synthesize butanol which was then condensed with acetyl-CoA through an alcohol acetyltransferase. Optimization of alcohol acetyltransferase expression and redox balance with auto-inducible fermentative controlled gene expression led to an effective titer of 22.8 ± 1.8 g/L butyl acetate produced in a bench-top bioreactor. CONCLUSION: Building on the well-developed Clostridium CoA-dependent butanol biosynthetic pathway, expression of an alcohol acetyltransferase converts the butanol produced into butyl acetate. The results from this study provided a strain of E. coli capable of directly producing butyl acetate from renewable resources at ambient conditions. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12934-022-01755-y. BioMed Central 2022-02-22 /pmc/articles/PMC8864926/ /pubmed/35193559 http://dx.doi.org/10.1186/s12934-022-01755-y Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
spellingShingle | Research Ku, Jason T. Chen, Arvin Y. Lan, Ethan I. Metabolic engineering of Escherichia coli for efficient biosynthesis of butyl acetate |
title | Metabolic engineering of Escherichia coli for efficient biosynthesis of butyl acetate |
title_full | Metabolic engineering of Escherichia coli for efficient biosynthesis of butyl acetate |
title_fullStr | Metabolic engineering of Escherichia coli for efficient biosynthesis of butyl acetate |
title_full_unstemmed | Metabolic engineering of Escherichia coli for efficient biosynthesis of butyl acetate |
title_short | Metabolic engineering of Escherichia coli for efficient biosynthesis of butyl acetate |
title_sort | metabolic engineering of escherichia coli for efficient biosynthesis of butyl acetate |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8864926/ https://www.ncbi.nlm.nih.gov/pubmed/35193559 http://dx.doi.org/10.1186/s12934-022-01755-y |
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