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The potential of fecal microbiota and amino acids to detect and monitor patients with adenoma

The risk of recurrent dysplastic colonic lesions is increased following polypectomy. Yield of endoscopic surveillance after adenoma removal is low, while interval colorectal cancers occur. To longitudinally assess the dynamics of fecal microbiota and amino acids in the presence of adenomatous lesion...

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Autores principales: Bosch, Sofie, Acharjee, Animesh, Quraishi, Mohammed N, Rojas, Patricia, Bakkali, Abdellatif, Jansen, Erwin EW, Brizzio Brentar, Marina, Kuijvenhoven, Johan, Stokkers, Pieter, Struys, Eduard, Beggs, Andrew D, Gkoutos, Georgios V, de Meij, Tim GJ, de Boer, Nanne KH
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taylor & Francis 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8865277/
https://www.ncbi.nlm.nih.gov/pubmed/35188868
http://dx.doi.org/10.1080/19490976.2022.2038863
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author Bosch, Sofie
Acharjee, Animesh
Quraishi, Mohammed N
Rojas, Patricia
Bakkali, Abdellatif
Jansen, Erwin EW
Brizzio Brentar, Marina
Kuijvenhoven, Johan
Stokkers, Pieter
Struys, Eduard
Beggs, Andrew D
Gkoutos, Georgios V
de Meij, Tim GJ
de Boer, Nanne KH
author_facet Bosch, Sofie
Acharjee, Animesh
Quraishi, Mohammed N
Rojas, Patricia
Bakkali, Abdellatif
Jansen, Erwin EW
Brizzio Brentar, Marina
Kuijvenhoven, Johan
Stokkers, Pieter
Struys, Eduard
Beggs, Andrew D
Gkoutos, Georgios V
de Meij, Tim GJ
de Boer, Nanne KH
author_sort Bosch, Sofie
collection PubMed
description The risk of recurrent dysplastic colonic lesions is increased following polypectomy. Yield of endoscopic surveillance after adenoma removal is low, while interval colorectal cancers occur. To longitudinally assess the dynamics of fecal microbiota and amino acids in the presence of adenomatous lesions and after their endoscopic removal. In this longitudinal case–control study, patients collected fecal samples prior to bowel preparation before scheduled colonoscopy and 3 months after this intervention. Based on colonoscopy outcomes, patients with advanced adenomas and nonadvanced adenomas (0.5–1.0 cm) who underwent polypectomy during endoscopy (n = 19) were strictly matched on age, body-mass index, and smoking habits to controls without endoscopic abnormalities (n = 19). Microbial taxa were measured by 16S RNA sequencing, and amino acids (AA) were measured by high-performance liquid chromatography (HPLC). Adenoma patients were discriminated from controls based on AA and microbial composition. Levels of proline (p = .001), ornithine (p = .02) and serine (p = .02) were increased in adenoma patients compared to controls but decreased to resemble those of controls after adenoma removal. These AAs were combined as a potential adenoma-specific panel (AUC 0.79(0.64–0.94)). For bacterial taxa, differences between patients with adenomas and controls were found (Bifidobacterium spp.↓, Anaerostipes spp.↓, Butyricimonas spp.↑, Faecalitalea spp.↑ and Catenibacterium spp.↑), but no alterations in relative abundance were observed after polypectomy. Furthermore, Faecalitalea spp. and Butyricimonas spp. were significantly correlated with adenoma-specific amino acids. We selected an amino acid panel specifically increased in the presence of adenomas and a microbial signature present in adenoma patients, irrespective of polypectomy. Upon validation, these panels may improve the effectiveness of the surveillance program by detection of high-risk individuals and determination of surveillance endoscopy timing, leading to less unnecessary endoscopies and less interval cancer.
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spelling pubmed-88652772022-02-24 The potential of fecal microbiota and amino acids to detect and monitor patients with adenoma Bosch, Sofie Acharjee, Animesh Quraishi, Mohammed N Rojas, Patricia Bakkali, Abdellatif Jansen, Erwin EW Brizzio Brentar, Marina Kuijvenhoven, Johan Stokkers, Pieter Struys, Eduard Beggs, Andrew D Gkoutos, Georgios V de Meij, Tim GJ de Boer, Nanne KH Gut Microbes Research Paper The risk of recurrent dysplastic colonic lesions is increased following polypectomy. Yield of endoscopic surveillance after adenoma removal is low, while interval colorectal cancers occur. To longitudinally assess the dynamics of fecal microbiota and amino acids in the presence of adenomatous lesions and after their endoscopic removal. In this longitudinal case–control study, patients collected fecal samples prior to bowel preparation before scheduled colonoscopy and 3 months after this intervention. Based on colonoscopy outcomes, patients with advanced adenomas and nonadvanced adenomas (0.5–1.0 cm) who underwent polypectomy during endoscopy (n = 19) were strictly matched on age, body-mass index, and smoking habits to controls without endoscopic abnormalities (n = 19). Microbial taxa were measured by 16S RNA sequencing, and amino acids (AA) were measured by high-performance liquid chromatography (HPLC). Adenoma patients were discriminated from controls based on AA and microbial composition. Levels of proline (p = .001), ornithine (p = .02) and serine (p = .02) were increased in adenoma patients compared to controls but decreased to resemble those of controls after adenoma removal. These AAs were combined as a potential adenoma-specific panel (AUC 0.79(0.64–0.94)). For bacterial taxa, differences between patients with adenomas and controls were found (Bifidobacterium spp.↓, Anaerostipes spp.↓, Butyricimonas spp.↑, Faecalitalea spp.↑ and Catenibacterium spp.↑), but no alterations in relative abundance were observed after polypectomy. Furthermore, Faecalitalea spp. and Butyricimonas spp. were significantly correlated with adenoma-specific amino acids. We selected an amino acid panel specifically increased in the presence of adenomas and a microbial signature present in adenoma patients, irrespective of polypectomy. Upon validation, these panels may improve the effectiveness of the surveillance program by detection of high-risk individuals and determination of surveillance endoscopy timing, leading to less unnecessary endoscopies and less interval cancer. Taylor & Francis 2022-02-21 /pmc/articles/PMC8865277/ /pubmed/35188868 http://dx.doi.org/10.1080/19490976.2022.2038863 Text en © 2022 The Author(s). Published with license by Taylor & Francis Group, LLC. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Paper
Bosch, Sofie
Acharjee, Animesh
Quraishi, Mohammed N
Rojas, Patricia
Bakkali, Abdellatif
Jansen, Erwin EW
Brizzio Brentar, Marina
Kuijvenhoven, Johan
Stokkers, Pieter
Struys, Eduard
Beggs, Andrew D
Gkoutos, Georgios V
de Meij, Tim GJ
de Boer, Nanne KH
The potential of fecal microbiota and amino acids to detect and monitor patients with adenoma
title The potential of fecal microbiota and amino acids to detect and monitor patients with adenoma
title_full The potential of fecal microbiota and amino acids to detect and monitor patients with adenoma
title_fullStr The potential of fecal microbiota and amino acids to detect and monitor patients with adenoma
title_full_unstemmed The potential of fecal microbiota and amino acids to detect and monitor patients with adenoma
title_short The potential of fecal microbiota and amino acids to detect and monitor patients with adenoma
title_sort potential of fecal microbiota and amino acids to detect and monitor patients with adenoma
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8865277/
https://www.ncbi.nlm.nih.gov/pubmed/35188868
http://dx.doi.org/10.1080/19490976.2022.2038863
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