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Decay and damage of therapeutic phage OMKO1 by environmental stressors

Antibiotic resistant bacterial pathogens are increasingly prevalent, driving the need for alternative approaches to chemical antibiotics when treating infections. One such approach is bacteriophage therapy: the use of bacteria-specific viruses that lyse (kill) their host cells. Just as the effect of...

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Autores principales: Blazanin, Michael, Lam, Wai Tin, Vasen, Emma, Chan, Benjamin K., Turner, Paul E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8865689/
https://www.ncbi.nlm.nih.gov/pubmed/35196336
http://dx.doi.org/10.1371/journal.pone.0263887
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author Blazanin, Michael
Lam, Wai Tin
Vasen, Emma
Chan, Benjamin K.
Turner, Paul E.
author_facet Blazanin, Michael
Lam, Wai Tin
Vasen, Emma
Chan, Benjamin K.
Turner, Paul E.
author_sort Blazanin, Michael
collection PubMed
description Antibiotic resistant bacterial pathogens are increasingly prevalent, driving the need for alternative approaches to chemical antibiotics when treating infections. One such approach is bacteriophage therapy: the use of bacteria-specific viruses that lyse (kill) their host cells. Just as the effect of environmental conditions (e.g. elevated temperature) on antibiotic efficacy is well-studied, the effect of environmental stressors on the potency of phage therapy candidates demands examination. Therapeutic phage OMKO1 infects and kills the opportunistic human pathogen Pseudomonas aeruginosa. Here, we used phage OMKO1 as a model to test how environmental stressors can lead to damage and decay of virus particles. We assessed the effects of elevated temperatures, saline concentrations, and urea concentrations. We observed that OMKO1 particles were highly tolerant to different saline concentrations, but decayed more rapidly at elevated temperatures and under high concentrations of urea. Additionally, we found that exposure to elevated temperature reduced the ability of surviving phage particles to suppress the growth of P. aeruginosa, suggesting a temperature-induced damage. Our findings demonstrate that OMKO1 is highly tolerant to a range of conditions that could be experienced inside and outside the human body, while also showing the need for careful characterization of therapeutic phages to ensure that environmental exposure does not compromise their expected potency, dosing, and pharmacokinetics.
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spelling pubmed-88656892022-02-24 Decay and damage of therapeutic phage OMKO1 by environmental stressors Blazanin, Michael Lam, Wai Tin Vasen, Emma Chan, Benjamin K. Turner, Paul E. PLoS One Research Article Antibiotic resistant bacterial pathogens are increasingly prevalent, driving the need for alternative approaches to chemical antibiotics when treating infections. One such approach is bacteriophage therapy: the use of bacteria-specific viruses that lyse (kill) their host cells. Just as the effect of environmental conditions (e.g. elevated temperature) on antibiotic efficacy is well-studied, the effect of environmental stressors on the potency of phage therapy candidates demands examination. Therapeutic phage OMKO1 infects and kills the opportunistic human pathogen Pseudomonas aeruginosa. Here, we used phage OMKO1 as a model to test how environmental stressors can lead to damage and decay of virus particles. We assessed the effects of elevated temperatures, saline concentrations, and urea concentrations. We observed that OMKO1 particles were highly tolerant to different saline concentrations, but decayed more rapidly at elevated temperatures and under high concentrations of urea. Additionally, we found that exposure to elevated temperature reduced the ability of surviving phage particles to suppress the growth of P. aeruginosa, suggesting a temperature-induced damage. Our findings demonstrate that OMKO1 is highly tolerant to a range of conditions that could be experienced inside and outside the human body, while also showing the need for careful characterization of therapeutic phages to ensure that environmental exposure does not compromise their expected potency, dosing, and pharmacokinetics. Public Library of Science 2022-02-23 /pmc/articles/PMC8865689/ /pubmed/35196336 http://dx.doi.org/10.1371/journal.pone.0263887 Text en © 2022 Blazanin et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Blazanin, Michael
Lam, Wai Tin
Vasen, Emma
Chan, Benjamin K.
Turner, Paul E.
Decay and damage of therapeutic phage OMKO1 by environmental stressors
title Decay and damage of therapeutic phage OMKO1 by environmental stressors
title_full Decay and damage of therapeutic phage OMKO1 by environmental stressors
title_fullStr Decay and damage of therapeutic phage OMKO1 by environmental stressors
title_full_unstemmed Decay and damage of therapeutic phage OMKO1 by environmental stressors
title_short Decay and damage of therapeutic phage OMKO1 by environmental stressors
title_sort decay and damage of therapeutic phage omko1 by environmental stressors
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8865689/
https://www.ncbi.nlm.nih.gov/pubmed/35196336
http://dx.doi.org/10.1371/journal.pone.0263887
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