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Decay and damage of therapeutic phage OMKO1 by environmental stressors
Antibiotic resistant bacterial pathogens are increasingly prevalent, driving the need for alternative approaches to chemical antibiotics when treating infections. One such approach is bacteriophage therapy: the use of bacteria-specific viruses that lyse (kill) their host cells. Just as the effect of...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8865689/ https://www.ncbi.nlm.nih.gov/pubmed/35196336 http://dx.doi.org/10.1371/journal.pone.0263887 |
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author | Blazanin, Michael Lam, Wai Tin Vasen, Emma Chan, Benjamin K. Turner, Paul E. |
author_facet | Blazanin, Michael Lam, Wai Tin Vasen, Emma Chan, Benjamin K. Turner, Paul E. |
author_sort | Blazanin, Michael |
collection | PubMed |
description | Antibiotic resistant bacterial pathogens are increasingly prevalent, driving the need for alternative approaches to chemical antibiotics when treating infections. One such approach is bacteriophage therapy: the use of bacteria-specific viruses that lyse (kill) their host cells. Just as the effect of environmental conditions (e.g. elevated temperature) on antibiotic efficacy is well-studied, the effect of environmental stressors on the potency of phage therapy candidates demands examination. Therapeutic phage OMKO1 infects and kills the opportunistic human pathogen Pseudomonas aeruginosa. Here, we used phage OMKO1 as a model to test how environmental stressors can lead to damage and decay of virus particles. We assessed the effects of elevated temperatures, saline concentrations, and urea concentrations. We observed that OMKO1 particles were highly tolerant to different saline concentrations, but decayed more rapidly at elevated temperatures and under high concentrations of urea. Additionally, we found that exposure to elevated temperature reduced the ability of surviving phage particles to suppress the growth of P. aeruginosa, suggesting a temperature-induced damage. Our findings demonstrate that OMKO1 is highly tolerant to a range of conditions that could be experienced inside and outside the human body, while also showing the need for careful characterization of therapeutic phages to ensure that environmental exposure does not compromise their expected potency, dosing, and pharmacokinetics. |
format | Online Article Text |
id | pubmed-8865689 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-88656892022-02-24 Decay and damage of therapeutic phage OMKO1 by environmental stressors Blazanin, Michael Lam, Wai Tin Vasen, Emma Chan, Benjamin K. Turner, Paul E. PLoS One Research Article Antibiotic resistant bacterial pathogens are increasingly prevalent, driving the need for alternative approaches to chemical antibiotics when treating infections. One such approach is bacteriophage therapy: the use of bacteria-specific viruses that lyse (kill) their host cells. Just as the effect of environmental conditions (e.g. elevated temperature) on antibiotic efficacy is well-studied, the effect of environmental stressors on the potency of phage therapy candidates demands examination. Therapeutic phage OMKO1 infects and kills the opportunistic human pathogen Pseudomonas aeruginosa. Here, we used phage OMKO1 as a model to test how environmental stressors can lead to damage and decay of virus particles. We assessed the effects of elevated temperatures, saline concentrations, and urea concentrations. We observed that OMKO1 particles were highly tolerant to different saline concentrations, but decayed more rapidly at elevated temperatures and under high concentrations of urea. Additionally, we found that exposure to elevated temperature reduced the ability of surviving phage particles to suppress the growth of P. aeruginosa, suggesting a temperature-induced damage. Our findings demonstrate that OMKO1 is highly tolerant to a range of conditions that could be experienced inside and outside the human body, while also showing the need for careful characterization of therapeutic phages to ensure that environmental exposure does not compromise their expected potency, dosing, and pharmacokinetics. Public Library of Science 2022-02-23 /pmc/articles/PMC8865689/ /pubmed/35196336 http://dx.doi.org/10.1371/journal.pone.0263887 Text en © 2022 Blazanin et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Blazanin, Michael Lam, Wai Tin Vasen, Emma Chan, Benjamin K. Turner, Paul E. Decay and damage of therapeutic phage OMKO1 by environmental stressors |
title | Decay and damage of therapeutic phage OMKO1 by environmental stressors |
title_full | Decay and damage of therapeutic phage OMKO1 by environmental stressors |
title_fullStr | Decay and damage of therapeutic phage OMKO1 by environmental stressors |
title_full_unstemmed | Decay and damage of therapeutic phage OMKO1 by environmental stressors |
title_short | Decay and damage of therapeutic phage OMKO1 by environmental stressors |
title_sort | decay and damage of therapeutic phage omko1 by environmental stressors |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8865689/ https://www.ncbi.nlm.nih.gov/pubmed/35196336 http://dx.doi.org/10.1371/journal.pone.0263887 |
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