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Modeling the temporal dynamics of master regulators and CtrA proteolysis in Caulobacter crescentus cell cycle

The cell cycle of Caulobacter crescentus involves the polar morphogenesis and an asymmetric cell division driven by precise interactions and regulations of proteins, which makes Caulobacter an ideal model organism for investigating bacterial cell development and differentiation. The abundance of mol...

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Autores principales: Xu, Chunrui, Hollis, Henry, Dai, Michelle, Yao, Xiangyu, Watson, Layne T., Cao, Yang, Chen, Minghan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8865702/
https://www.ncbi.nlm.nih.gov/pubmed/35089921
http://dx.doi.org/10.1371/journal.pcbi.1009847
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author Xu, Chunrui
Hollis, Henry
Dai, Michelle
Yao, Xiangyu
Watson, Layne T.
Cao, Yang
Chen, Minghan
author_facet Xu, Chunrui
Hollis, Henry
Dai, Michelle
Yao, Xiangyu
Watson, Layne T.
Cao, Yang
Chen, Minghan
author_sort Xu, Chunrui
collection PubMed
description The cell cycle of Caulobacter crescentus involves the polar morphogenesis and an asymmetric cell division driven by precise interactions and regulations of proteins, which makes Caulobacter an ideal model organism for investigating bacterial cell development and differentiation. The abundance of molecular data accumulated on Caulobacter motivates system biologists to analyze the complex regulatory network of cell cycle via quantitative modeling. In this paper, We propose a comprehensive model to accurately characterize the underlying mechanisms of cell cycle regulation based on the study of: a) chromosome replication and methylation; b) interactive pathways of five master regulatory proteins including DnaA, GcrA, CcrM, CtrA, and SciP, as well as novel consideration of their corresponding mRNAs; c) cell cycle-dependent proteolysis of CtrA through hierarchical protease complexes. The temporal dynamics of our simulation results are able to closely replicate an extensive set of experimental observations and capture the main phenotype of seven mutant strains of Caulobacter crescentus. Collectively, the proposed model can be used to predict phenotypes of other mutant cases, especially for nonviable strains which are hard to cultivate and observe. Moreover, the module of cyclic proteolysis is an efficient tool to study the metabolism of proteins with similar mechanisms.
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spelling pubmed-88657022022-02-24 Modeling the temporal dynamics of master regulators and CtrA proteolysis in Caulobacter crescentus cell cycle Xu, Chunrui Hollis, Henry Dai, Michelle Yao, Xiangyu Watson, Layne T. Cao, Yang Chen, Minghan PLoS Comput Biol Research Article The cell cycle of Caulobacter crescentus involves the polar morphogenesis and an asymmetric cell division driven by precise interactions and regulations of proteins, which makes Caulobacter an ideal model organism for investigating bacterial cell development and differentiation. The abundance of molecular data accumulated on Caulobacter motivates system biologists to analyze the complex regulatory network of cell cycle via quantitative modeling. In this paper, We propose a comprehensive model to accurately characterize the underlying mechanisms of cell cycle regulation based on the study of: a) chromosome replication and methylation; b) interactive pathways of five master regulatory proteins including DnaA, GcrA, CcrM, CtrA, and SciP, as well as novel consideration of their corresponding mRNAs; c) cell cycle-dependent proteolysis of CtrA through hierarchical protease complexes. The temporal dynamics of our simulation results are able to closely replicate an extensive set of experimental observations and capture the main phenotype of seven mutant strains of Caulobacter crescentus. Collectively, the proposed model can be used to predict phenotypes of other mutant cases, especially for nonviable strains which are hard to cultivate and observe. Moreover, the module of cyclic proteolysis is an efficient tool to study the metabolism of proteins with similar mechanisms. Public Library of Science 2022-01-28 /pmc/articles/PMC8865702/ /pubmed/35089921 http://dx.doi.org/10.1371/journal.pcbi.1009847 Text en © 2022 Xu et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Xu, Chunrui
Hollis, Henry
Dai, Michelle
Yao, Xiangyu
Watson, Layne T.
Cao, Yang
Chen, Minghan
Modeling the temporal dynamics of master regulators and CtrA proteolysis in Caulobacter crescentus cell cycle
title Modeling the temporal dynamics of master regulators and CtrA proteolysis in Caulobacter crescentus cell cycle
title_full Modeling the temporal dynamics of master regulators and CtrA proteolysis in Caulobacter crescentus cell cycle
title_fullStr Modeling the temporal dynamics of master regulators and CtrA proteolysis in Caulobacter crescentus cell cycle
title_full_unstemmed Modeling the temporal dynamics of master regulators and CtrA proteolysis in Caulobacter crescentus cell cycle
title_short Modeling the temporal dynamics of master regulators and CtrA proteolysis in Caulobacter crescentus cell cycle
title_sort modeling the temporal dynamics of master regulators and ctra proteolysis in caulobacter crescentus cell cycle
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8865702/
https://www.ncbi.nlm.nih.gov/pubmed/35089921
http://dx.doi.org/10.1371/journal.pcbi.1009847
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