An efficient miRNA knockout approach using CRISPR-Cas9 in Xenopus()

In recent years CRISPR-Cas9 knockouts (KO) have become increasingly ultilised to study gene function. MicroRNAs (miRNAs) are short non-coding RNAs, 20–22 nucleotides long, which affect gene expression through post-transcriptional repression. We previously identified miRNAs-196a and −219 as implicate...

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Detalles Bibliográficos
Autores principales: Godden, Alice M., Antonaci, Marco, Ward, Nicole J., van der Lee, Michael, Abu-Daya, Anita, Guille, Matthew, Wheeler, Grant N.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8865746/
https://www.ncbi.nlm.nih.gov/pubmed/34968443
http://dx.doi.org/10.1016/j.ydbio.2021.12.015

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