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Pharmacological Mechanisms of Shangke Huangshui against Skin and Soft Tissue Infection

BACKGROUND: Skin and soft tissue infections (SSTIs) are a group of common diseases, usually caused by bacteria. Shangke Huangshui (SK) has been widely used to treat various SSTIs diseases for many years, but its mechanism is unclear. Therefore, this study was designed to investigate the anti-infecti...

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Autores principales: Chen, Yanfen, Li, Mengqiu, Zheng, Fanghao, Jiang, Wei, Lei, Kaijun, Li, Huaiguo, Liu, Dongwen, Zhang, Bairong, He, Mingfeng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8865977/
https://www.ncbi.nlm.nih.gov/pubmed/35222679
http://dx.doi.org/10.1155/2022/9312611
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author Chen, Yanfen
Li, Mengqiu
Zheng, Fanghao
Jiang, Wei
Lei, Kaijun
Li, Huaiguo
Liu, Dongwen
Zhang, Bairong
He, Mingfeng
author_facet Chen, Yanfen
Li, Mengqiu
Zheng, Fanghao
Jiang, Wei
Lei, Kaijun
Li, Huaiguo
Liu, Dongwen
Zhang, Bairong
He, Mingfeng
author_sort Chen, Yanfen
collection PubMed
description BACKGROUND: Skin and soft tissue infections (SSTIs) are a group of common diseases, usually caused by bacteria. Shangke Huangshui (SK) has been widely used to treat various SSTIs diseases for many years, but its mechanism is unclear. Therefore, this study was designed to investigate the anti-infective effect of SK on different skin and soft tissue infection diseases and to explore its underlying mechanism. METHODS: The subcutaneous abscess mouse model, the dermal ulcer rat model, and the infectious soft tissue injury rat model were established by injection of Staphylococcus aureus to evaluate the anti-inflammatory and antibacterial effects of SK. Abscess volume, local appearance score and histological changes, bacterial contents, and hydroxyproline concentration in the skin or soft tissue were analyzed. The levels of NO, TNF-α, IL-1β, and IL-8 in the serum and tissue were determined by ELISA method. The mRNA expression levels of TLR2, MyD88, TAK1, NF-κB, AP-1, and other genes were measured with qRT-PCR method, and the protein expression of TLR2, MyD88, TAK1, NF-κB, and AP-1 was detected by western blot method. RESULTS: SK had an obvious therapeutic effect on skin and soft tissue infections. It reduced the volume of abscess and promoted the healing of skin ulcer, improved pathological phenomena, such as inflammatory infiltration of skin and soft tissue, reduced the levels of white blood cells and NO in the blood, decreased bacteria contents in the skin and soft tissue. Furthermore, SK decreased the mRNA expression of TLR2, MyD88, TAK1, NF-κB and AP-1, and other related genes and also downregulated the protein expression of TLR2, MyD88, TAK1, NF-κB, and AP-1. CONCLUSION: The experiments provide evidence that SK can treat skin and soft tissue infection diseases based on its comprehensive antibacterial and anti-inflammatory effects. The therapeutic mechanism may be associated with the inhibition of TLR2/MyD88/NF-κB signaling pathway.
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spelling pubmed-88659772022-02-24 Pharmacological Mechanisms of Shangke Huangshui against Skin and Soft Tissue Infection Chen, Yanfen Li, Mengqiu Zheng, Fanghao Jiang, Wei Lei, Kaijun Li, Huaiguo Liu, Dongwen Zhang, Bairong He, Mingfeng Evid Based Complement Alternat Med Research Article BACKGROUND: Skin and soft tissue infections (SSTIs) are a group of common diseases, usually caused by bacteria. Shangke Huangshui (SK) has been widely used to treat various SSTIs diseases for many years, but its mechanism is unclear. Therefore, this study was designed to investigate the anti-infective effect of SK on different skin and soft tissue infection diseases and to explore its underlying mechanism. METHODS: The subcutaneous abscess mouse model, the dermal ulcer rat model, and the infectious soft tissue injury rat model were established by injection of Staphylococcus aureus to evaluate the anti-inflammatory and antibacterial effects of SK. Abscess volume, local appearance score and histological changes, bacterial contents, and hydroxyproline concentration in the skin or soft tissue were analyzed. The levels of NO, TNF-α, IL-1β, and IL-8 in the serum and tissue were determined by ELISA method. The mRNA expression levels of TLR2, MyD88, TAK1, NF-κB, AP-1, and other genes were measured with qRT-PCR method, and the protein expression of TLR2, MyD88, TAK1, NF-κB, and AP-1 was detected by western blot method. RESULTS: SK had an obvious therapeutic effect on skin and soft tissue infections. It reduced the volume of abscess and promoted the healing of skin ulcer, improved pathological phenomena, such as inflammatory infiltration of skin and soft tissue, reduced the levels of white blood cells and NO in the blood, decreased bacteria contents in the skin and soft tissue. Furthermore, SK decreased the mRNA expression of TLR2, MyD88, TAK1, NF-κB and AP-1, and other related genes and also downregulated the protein expression of TLR2, MyD88, TAK1, NF-κB, and AP-1. CONCLUSION: The experiments provide evidence that SK can treat skin and soft tissue infection diseases based on its comprehensive antibacterial and anti-inflammatory effects. The therapeutic mechanism may be associated with the inhibition of TLR2/MyD88/NF-κB signaling pathway. Hindawi 2022-02-16 /pmc/articles/PMC8865977/ /pubmed/35222679 http://dx.doi.org/10.1155/2022/9312611 Text en Copyright © 2022 Yanfen Chen et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Chen, Yanfen
Li, Mengqiu
Zheng, Fanghao
Jiang, Wei
Lei, Kaijun
Li, Huaiguo
Liu, Dongwen
Zhang, Bairong
He, Mingfeng
Pharmacological Mechanisms of Shangke Huangshui against Skin and Soft Tissue Infection
title Pharmacological Mechanisms of Shangke Huangshui against Skin and Soft Tissue Infection
title_full Pharmacological Mechanisms of Shangke Huangshui against Skin and Soft Tissue Infection
title_fullStr Pharmacological Mechanisms of Shangke Huangshui against Skin and Soft Tissue Infection
title_full_unstemmed Pharmacological Mechanisms of Shangke Huangshui against Skin and Soft Tissue Infection
title_short Pharmacological Mechanisms of Shangke Huangshui against Skin and Soft Tissue Infection
title_sort pharmacological mechanisms of shangke huangshui against skin and soft tissue infection
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8865977/
https://www.ncbi.nlm.nih.gov/pubmed/35222679
http://dx.doi.org/10.1155/2022/9312611
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