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Protocol to identify and monitor key mutations of broadly neutralizing antibody lineages following sequential immunization of Ig-humanized mice

Using the VRC01-class of anti-HIV-1 broadly neutralizing antibodies (bnAbs) elicited in sequentially immunized Ig-humanized mice as an example, we describe a protocol to identify key mutations for bnAb function by point mutagenesis and antibody binding and neutralization assays. We also describe ste...

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Detalles Bibliográficos
Autores principales:	Chen, Xuejun, Schmidt, Stephen D., Duan, Hongying, Doria-Rose, Nicole A., Mascola, John R.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Elsevier 2022
Materias:	Protocol
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8866138/ https://www.ncbi.nlm.nih.gov/pubmed/35243372 http://dx.doi.org/10.1016/j.xpro.2022.101180

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