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Sulforaphane Exerts Beneficial Immunomodulatory Effects on Liver Tissue via a Nrf2 Pathway-Related Mechanism in a Murine Model of Hemorrhagic Shock and Resuscitation

Our research explores the immunomodulatory effects of sulforaphane (SFN), a well-known nuclear factor erythroid 2-related factor 2 (Nrf2) pathway agonist, on the sterile inflammation of and ischemia-reperfusion injuries to the liver after hemorrhagic shock (HS) followed by resuscitation (R). Male C5...

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Autores principales: Liang, Weiqiang, Greven, Johannes, Qin, Kang, Fragoulis, Athanassios, Horst, Klemens, Bläsius, Felix, Wruck, Christoph, Pufe, Thomas, Kobbe, Philipp, Hildebrand, Frank, Lichte, Philipp
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8866169/
https://www.ncbi.nlm.nih.gov/pubmed/35222401
http://dx.doi.org/10.3389/fimmu.2022.822895
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author Liang, Weiqiang
Greven, Johannes
Qin, Kang
Fragoulis, Athanassios
Horst, Klemens
Bläsius, Felix
Wruck, Christoph
Pufe, Thomas
Kobbe, Philipp
Hildebrand, Frank
Lichte, Philipp
author_facet Liang, Weiqiang
Greven, Johannes
Qin, Kang
Fragoulis, Athanassios
Horst, Klemens
Bläsius, Felix
Wruck, Christoph
Pufe, Thomas
Kobbe, Philipp
Hildebrand, Frank
Lichte, Philipp
author_sort Liang, Weiqiang
collection PubMed
description Our research explores the immunomodulatory effects of sulforaphane (SFN), a well-known nuclear factor erythroid 2-related factor 2 (Nrf2) pathway agonist, on the sterile inflammation of and ischemia-reperfusion injuries to the liver after hemorrhagic shock (HS) followed by resuscitation (R). Male C57/BL6 wild-type and transgenic ARE-luc mice were exposed to mean arterial pressure-controlled HS. Fluid resuscitation was performed after 90 min of HS, and SFN was administrated intraperitoneally after that. The animals were sacrificed at 6 h, 24 h, and 72 h after resuscitation, and their livers were extracted to perform H&E staining and myeloperoxidase (MPO) activity analysis. The Kupffer cells were isolated for cytokines profile measurements and Nrf2 immunofluorescence staining. Further, the ARE-luc mice were used to assess hepatic Nrf2 activity in vivo. We identified that SFN-activated Kupffer cells’ Nrf2 pathway and modulated its cytokines expression, including TNF-α, MCP-1, KC/CXCL1, IL-6, and IL-10. Furthermore, SFN mitigated liver ischemia-reperfusion injury, as evidenced by the downregulation of the Suzuki score and the enhanced hepatic Nrf2 activity. The in vivo SFN treatment decreased neutrophils infiltration, as shown by the decreased MPO levels. Our study shows that SFN can decrease HS/R-induced hepatic ischemia-reperfusion injury and modulate the activity of Kupffer cells via an Nrf2-dependent pathway.
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spelling pubmed-88661692022-02-25 Sulforaphane Exerts Beneficial Immunomodulatory Effects on Liver Tissue via a Nrf2 Pathway-Related Mechanism in a Murine Model of Hemorrhagic Shock and Resuscitation Liang, Weiqiang Greven, Johannes Qin, Kang Fragoulis, Athanassios Horst, Klemens Bläsius, Felix Wruck, Christoph Pufe, Thomas Kobbe, Philipp Hildebrand, Frank Lichte, Philipp Front Immunol Immunology Our research explores the immunomodulatory effects of sulforaphane (SFN), a well-known nuclear factor erythroid 2-related factor 2 (Nrf2) pathway agonist, on the sterile inflammation of and ischemia-reperfusion injuries to the liver after hemorrhagic shock (HS) followed by resuscitation (R). Male C57/BL6 wild-type and transgenic ARE-luc mice were exposed to mean arterial pressure-controlled HS. Fluid resuscitation was performed after 90 min of HS, and SFN was administrated intraperitoneally after that. The animals were sacrificed at 6 h, 24 h, and 72 h after resuscitation, and their livers were extracted to perform H&E staining and myeloperoxidase (MPO) activity analysis. The Kupffer cells were isolated for cytokines profile measurements and Nrf2 immunofluorescence staining. Further, the ARE-luc mice were used to assess hepatic Nrf2 activity in vivo. We identified that SFN-activated Kupffer cells’ Nrf2 pathway and modulated its cytokines expression, including TNF-α, MCP-1, KC/CXCL1, IL-6, and IL-10. Furthermore, SFN mitigated liver ischemia-reperfusion injury, as evidenced by the downregulation of the Suzuki score and the enhanced hepatic Nrf2 activity. The in vivo SFN treatment decreased neutrophils infiltration, as shown by the decreased MPO levels. Our study shows that SFN can decrease HS/R-induced hepatic ischemia-reperfusion injury and modulate the activity of Kupffer cells via an Nrf2-dependent pathway. Frontiers Media S.A. 2022-02-10 /pmc/articles/PMC8866169/ /pubmed/35222401 http://dx.doi.org/10.3389/fimmu.2022.822895 Text en Copyright © 2022 Liang, Greven, Qin, Fragoulis, Horst, Bläsius, Wruck, Pufe, Kobbe, Hildebrand and Lichte https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Immunology
Liang, Weiqiang
Greven, Johannes
Qin, Kang
Fragoulis, Athanassios
Horst, Klemens
Bläsius, Felix
Wruck, Christoph
Pufe, Thomas
Kobbe, Philipp
Hildebrand, Frank
Lichte, Philipp
Sulforaphane Exerts Beneficial Immunomodulatory Effects on Liver Tissue via a Nrf2 Pathway-Related Mechanism in a Murine Model of Hemorrhagic Shock and Resuscitation
title Sulforaphane Exerts Beneficial Immunomodulatory Effects on Liver Tissue via a Nrf2 Pathway-Related Mechanism in a Murine Model of Hemorrhagic Shock and Resuscitation
title_full Sulforaphane Exerts Beneficial Immunomodulatory Effects on Liver Tissue via a Nrf2 Pathway-Related Mechanism in a Murine Model of Hemorrhagic Shock and Resuscitation
title_fullStr Sulforaphane Exerts Beneficial Immunomodulatory Effects on Liver Tissue via a Nrf2 Pathway-Related Mechanism in a Murine Model of Hemorrhagic Shock and Resuscitation
title_full_unstemmed Sulforaphane Exerts Beneficial Immunomodulatory Effects on Liver Tissue via a Nrf2 Pathway-Related Mechanism in a Murine Model of Hemorrhagic Shock and Resuscitation
title_short Sulforaphane Exerts Beneficial Immunomodulatory Effects on Liver Tissue via a Nrf2 Pathway-Related Mechanism in a Murine Model of Hemorrhagic Shock and Resuscitation
title_sort sulforaphane exerts beneficial immunomodulatory effects on liver tissue via a nrf2 pathway-related mechanism in a murine model of hemorrhagic shock and resuscitation
topic Immunology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8866169/
https://www.ncbi.nlm.nih.gov/pubmed/35222401
http://dx.doi.org/10.3389/fimmu.2022.822895
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