An adjuvanted subunit SARS-CoV-2 spike protein vaccine provides protection against Covid-19 infection and transmission

Recombinant protein approaches offer major promise for safe and effective vaccine prevention of SARS-CoV-2 infection. We developed a recombinant spike protein vaccine (called NARUVAX-C19) and characterized its ability when formulated with a nanoemulsion adjuvant to induce anti-spike antibody and T-c...

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Autores principales: Tabynov, Kairat, Turebekov, Nurkeldi, Babayeva, Meruert, Fomin, Gleb, Yerubayev, Toktassyn, Yespolov, Tlektes, Li, Lei, Renukaradhya, Gourapura J., Petrovsky, Nikolai, Tabynov, Kaissar
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
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Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8866462/
https://www.ncbi.nlm.nih.gov/pubmed/35197485
http://dx.doi.org/10.1038/s41541-022-00450-8
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author Tabynov, Kairat
Turebekov, Nurkeldi
Babayeva, Meruert
Fomin, Gleb
Yerubayev, Toktassyn
Yespolov, Tlektes
Li, Lei
Renukaradhya, Gourapura J.
Petrovsky, Nikolai
Tabynov, Kaissar
author_facet Tabynov, Kairat
Turebekov, Nurkeldi
Babayeva, Meruert
Fomin, Gleb
Yerubayev, Toktassyn
Yespolov, Tlektes
Li, Lei
Renukaradhya, Gourapura J.
Petrovsky, Nikolai
Tabynov, Kaissar
author_sort Tabynov, Kairat
collection PubMed
description Recombinant protein approaches offer major promise for safe and effective vaccine prevention of SARS-CoV-2 infection. We developed a recombinant spike protein vaccine (called NARUVAX-C19) and characterized its ability when formulated with a nanoemulsion adjuvant to induce anti-spike antibody and T-cell responses and provide protection including against viral transmission in rodent. In mice, NARUVAX-C19 vaccine administered intramuscularly twice at 21-day interval elicited balanced Th1/Th2 humoral and T-cell responses with high titers of neutralizing antibodies against wild-type (D614G) and delta (B.1.617.2) variants. In Syrian hamsters, NARUVAX-C19 provided complete protection against wild-type (D614G) infection and prevented its transmission to naïve animals (n = 2/group) placed in the same cage as challenged animals (n = 6/group). The results contrasted with only weak protection seen with a monomeric spike receptor-binding domain (RBD) vaccine even when formulated with the same adjuvant. These encouraging results warrant the ongoing development of this COVID-19 vaccine candidate.
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spelling pubmed-88664622022-03-17 An adjuvanted subunit SARS-CoV-2 spike protein vaccine provides protection against Covid-19 infection and transmission Tabynov, Kairat Turebekov, Nurkeldi Babayeva, Meruert Fomin, Gleb Yerubayev, Toktassyn Yespolov, Tlektes Li, Lei Renukaradhya, Gourapura J. Petrovsky, Nikolai Tabynov, Kaissar NPJ Vaccines Article Recombinant protein approaches offer major promise for safe and effective vaccine prevention of SARS-CoV-2 infection. We developed a recombinant spike protein vaccine (called NARUVAX-C19) and characterized its ability when formulated with a nanoemulsion adjuvant to induce anti-spike antibody and T-cell responses and provide protection including against viral transmission in rodent. In mice, NARUVAX-C19 vaccine administered intramuscularly twice at 21-day interval elicited balanced Th1/Th2 humoral and T-cell responses with high titers of neutralizing antibodies against wild-type (D614G) and delta (B.1.617.2) variants. In Syrian hamsters, NARUVAX-C19 provided complete protection against wild-type (D614G) infection and prevented its transmission to naïve animals (n = 2/group) placed in the same cage as challenged animals (n = 6/group). The results contrasted with only weak protection seen with a monomeric spike receptor-binding domain (RBD) vaccine even when formulated with the same adjuvant. These encouraging results warrant the ongoing development of this COVID-19 vaccine candidate. Nature Publishing Group UK 2022-02-23 /pmc/articles/PMC8866462/ /pubmed/35197485 http://dx.doi.org/10.1038/s41541-022-00450-8 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Tabynov, Kairat
Turebekov, Nurkeldi
Babayeva, Meruert
Fomin, Gleb
Yerubayev, Toktassyn
Yespolov, Tlektes
Li, Lei
Renukaradhya, Gourapura J.
Petrovsky, Nikolai
Tabynov, Kaissar
An adjuvanted subunit SARS-CoV-2 spike protein vaccine provides protection against Covid-19 infection and transmission
title An adjuvanted subunit SARS-CoV-2 spike protein vaccine provides protection against Covid-19 infection and transmission
title_full An adjuvanted subunit SARS-CoV-2 spike protein vaccine provides protection against Covid-19 infection and transmission
title_fullStr An adjuvanted subunit SARS-CoV-2 spike protein vaccine provides protection against Covid-19 infection and transmission
title_full_unstemmed An adjuvanted subunit SARS-CoV-2 spike protein vaccine provides protection against Covid-19 infection and transmission
title_short An adjuvanted subunit SARS-CoV-2 spike protein vaccine provides protection against Covid-19 infection and transmission
title_sort adjuvanted subunit sars-cov-2 spike protein vaccine provides protection against covid-19 infection and transmission
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8866462/
https://www.ncbi.nlm.nih.gov/pubmed/35197485
http://dx.doi.org/10.1038/s41541-022-00450-8
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