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A Novel Biopsy Method Based on Bipolar Radiofrequency Biopsy Needles

Modern oncology increasingly relies on pathological, molecular, and genomic assessments of biopsied tumor tissue. However, the concern for bleeding complication and malignant seeding severely hinders the application of the biopsy tumor. Here, we developed a 16 G biopsy needle to contain two electrod...

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Autores principales: Wang, Huiyang, Bao, Haiwei, Yue, Lan, Jiang, Tian’an
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8866661/
https://www.ncbi.nlm.nih.gov/pubmed/35223520
http://dx.doi.org/10.3389/fonc.2022.838667
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author Wang, Huiyang
Bao, Haiwei
Yue, Lan
Jiang, Tian’an
author_facet Wang, Huiyang
Bao, Haiwei
Yue, Lan
Jiang, Tian’an
author_sort Wang, Huiyang
collection PubMed
description Modern oncology increasingly relies on pathological, molecular, and genomic assessments of biopsied tumor tissue. However, the concern for bleeding complication and malignant seeding severely hinders the application of the biopsy tumor. Here, we developed a 16 G biopsy needle to contain two electrodes insulated from each other and connect to an radiofrequency generator. For evaluating hemostatic efficacy, 50 rabbits were randomly divided into two groups: warfarinization and non-warfarinization group. Two liver biopsies and two splenic biopsies per animal were performed using a 16 G biopsy needle. Each group was further equally divided into five groups according to different hemostatic measures, including non-intervention, embolization using an absorbable gelatin sponge, and ablation by RF with three different needle temperatures (50°C, 70°C, and 90°C). Than, we used VX2 rabbit models (n = 25) and applied the five analogous biopsies to the tumor. The flush fluid from the biopsy needle underwent cytomorphological analysis. Our results that the groups using ablation by RF showed significantly less blood loss than the control group for liver and spleen in both groups (P < 0.001). After RF ablation, thermal coagulation of the tissue surrounding the needle tract was observed on both the macroscopic and histological level. Cytological smears showed that tumor cells were degenerated after RF at 70°C and 90°C. Our findings showed that bipolar RF biopsy needle is a promising tool for reducing hemorrhage after biopsy and avoiding implanting tumor cells in the tract.
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spelling pubmed-88666612022-02-25 A Novel Biopsy Method Based on Bipolar Radiofrequency Biopsy Needles Wang, Huiyang Bao, Haiwei Yue, Lan Jiang, Tian’an Front Oncol Oncology Modern oncology increasingly relies on pathological, molecular, and genomic assessments of biopsied tumor tissue. However, the concern for bleeding complication and malignant seeding severely hinders the application of the biopsy tumor. Here, we developed a 16 G biopsy needle to contain two electrodes insulated from each other and connect to an radiofrequency generator. For evaluating hemostatic efficacy, 50 rabbits were randomly divided into two groups: warfarinization and non-warfarinization group. Two liver biopsies and two splenic biopsies per animal were performed using a 16 G biopsy needle. Each group was further equally divided into five groups according to different hemostatic measures, including non-intervention, embolization using an absorbable gelatin sponge, and ablation by RF with three different needle temperatures (50°C, 70°C, and 90°C). Than, we used VX2 rabbit models (n = 25) and applied the five analogous biopsies to the tumor. The flush fluid from the biopsy needle underwent cytomorphological analysis. Our results that the groups using ablation by RF showed significantly less blood loss than the control group for liver and spleen in both groups (P < 0.001). After RF ablation, thermal coagulation of the tissue surrounding the needle tract was observed on both the macroscopic and histological level. Cytological smears showed that tumor cells were degenerated after RF at 70°C and 90°C. Our findings showed that bipolar RF biopsy needle is a promising tool for reducing hemorrhage after biopsy and avoiding implanting tumor cells in the tract. Frontiers Media S.A. 2022-02-10 /pmc/articles/PMC8866661/ /pubmed/35223520 http://dx.doi.org/10.3389/fonc.2022.838667 Text en Copyright © 2022 Wang, Bao, Yue and Jiang https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Oncology
Wang, Huiyang
Bao, Haiwei
Yue, Lan
Jiang, Tian’an
A Novel Biopsy Method Based on Bipolar Radiofrequency Biopsy Needles
title A Novel Biopsy Method Based on Bipolar Radiofrequency Biopsy Needles
title_full A Novel Biopsy Method Based on Bipolar Radiofrequency Biopsy Needles
title_fullStr A Novel Biopsy Method Based on Bipolar Radiofrequency Biopsy Needles
title_full_unstemmed A Novel Biopsy Method Based on Bipolar Radiofrequency Biopsy Needles
title_short A Novel Biopsy Method Based on Bipolar Radiofrequency Biopsy Needles
title_sort novel biopsy method based on bipolar radiofrequency biopsy needles
topic Oncology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8866661/
https://www.ncbi.nlm.nih.gov/pubmed/35223520
http://dx.doi.org/10.3389/fonc.2022.838667
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