The data of an all-solid-state acupuncture needle based potentiometric microelectrode for in vivo monitoring of calcium ions in rat cerebrospinal fluid

These data contain the details of the fabrication of the calcium ion-selective microelectrode (Ca(2+)-ISμEs) modified with poly(3,4-ethylenedioxythiophene)-poly(sodium 4-styrenesulfonate) (PEDOT(PSS)) as solid contact. The electrochemical impedance spectroscopy was carried out for the investigation of the resistance of the Ca(2+)-ISμEs. The thickness of the solid contact and the calcium ion-selective membrane was investigated by SEM. Potential-time curve of the electrodeposition of the PEDOT/PSS film onto the surface of the microelectrodes under the applied current of 0.5 μA for 200 s was recorded. The proposed Ca(2+)-ISμE was optimized through conditioning in different CaCl(2) solutions ranged from 1.0 × 10(−6) to 3.1 × 10(−3) M for different time before use. The anti-fouling property of the Ca(2+)-ISμEs against proteins was investigated through taking BSA as the model protein. The developed Ca(2+)-ISμEs were used for the in vivo monitoring of the calcium ions in rat cerebrospinal fluid under the stimuli of the spinal cord transection in a living animal.