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Pb(II)-phycoremediation mechanism using Scenedesmus obliquus: cells physicochemical properties and metabolomic profiling
This study highlights the mechanisms of Pb(II)-phycoremediation using the Pb(II) tolerant strain of Scenedesmus obliquus. First, monitoring of cell growth kinetics in control and Pb(II)-doped medium revealed significant growth inhibition, while the analyses through flow cytometry and Zetasizer revea...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8866896/ https://www.ncbi.nlm.nih.gov/pubmed/35243087 http://dx.doi.org/10.1016/j.heliyon.2022.e08967 |
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author | Danouche, M. El Ghachtouli, N. Aasfar, A. Bennis, I. El Arroussi, H. |
author_facet | Danouche, M. El Ghachtouli, N. Aasfar, A. Bennis, I. El Arroussi, H. |
author_sort | Danouche, M. |
collection | PubMed |
description | This study highlights the mechanisms of Pb(II)-phycoremediation using the Pb(II) tolerant strain of Scenedesmus obliquus. First, monitoring of cell growth kinetics in control and Pb(II)-doped medium revealed significant growth inhibition, while the analyses through flow cytometry and Zetasizer revealed no difference in cell viability and size. Residual weights of control and Pb(II)-loaded cells assessed by thermogravimetric analysis were 31.34% and 57.8%, respectively, indicating the uptake of Pb(II) into S. obliquus cells. Next, the use of chemical extraction to distinguish between the intracellular and extracellular uptake indicated the involvement of both biosorption (85.5%) and bioaccumulation (14.5%) mechanisms. Biosorption interaction of Pb(II) ions and the cell wall was confirmed using SEM-EDX, FTIR, zeta potential, zero-charge pH, and contact angle analyses. Besides, the biochemical characterization of control and Pb(II)-loaded cells revealed that the bioaccumulation of Pb(II) induces significant increases in the carotenoids and lipids content, while it decreases in the chlorophyll, carbohydrates, and proteins content. Finally, the metabolomic analysis indicated an increase in the relative abundance of fatty acid methyl esters, alkanes, aromatic compounds, and sterols. However, the alkenes and monounsaturated fatty acids decreased. Such metabolic adjustment may represent an adaptive strategy that prevents high Pb(II)-bioaccumulation in cellular compartments. |
format | Online Article Text |
id | pubmed-8866896 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-88668962022-03-02 Pb(II)-phycoremediation mechanism using Scenedesmus obliquus: cells physicochemical properties and metabolomic profiling Danouche, M. El Ghachtouli, N. Aasfar, A. Bennis, I. El Arroussi, H. Heliyon Research Article This study highlights the mechanisms of Pb(II)-phycoremediation using the Pb(II) tolerant strain of Scenedesmus obliquus. First, monitoring of cell growth kinetics in control and Pb(II)-doped medium revealed significant growth inhibition, while the analyses through flow cytometry and Zetasizer revealed no difference in cell viability and size. Residual weights of control and Pb(II)-loaded cells assessed by thermogravimetric analysis were 31.34% and 57.8%, respectively, indicating the uptake of Pb(II) into S. obliquus cells. Next, the use of chemical extraction to distinguish between the intracellular and extracellular uptake indicated the involvement of both biosorption (85.5%) and bioaccumulation (14.5%) mechanisms. Biosorption interaction of Pb(II) ions and the cell wall was confirmed using SEM-EDX, FTIR, zeta potential, zero-charge pH, and contact angle analyses. Besides, the biochemical characterization of control and Pb(II)-loaded cells revealed that the bioaccumulation of Pb(II) induces significant increases in the carotenoids and lipids content, while it decreases in the chlorophyll, carbohydrates, and proteins content. Finally, the metabolomic analysis indicated an increase in the relative abundance of fatty acid methyl esters, alkanes, aromatic compounds, and sterols. However, the alkenes and monounsaturated fatty acids decreased. Such metabolic adjustment may represent an adaptive strategy that prevents high Pb(II)-bioaccumulation in cellular compartments. Elsevier 2022-02-15 /pmc/articles/PMC8866896/ /pubmed/35243087 http://dx.doi.org/10.1016/j.heliyon.2022.e08967 Text en © 2022 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Research Article Danouche, M. El Ghachtouli, N. Aasfar, A. Bennis, I. El Arroussi, H. Pb(II)-phycoremediation mechanism using Scenedesmus obliquus: cells physicochemical properties and metabolomic profiling |
title | Pb(II)-phycoremediation mechanism using Scenedesmus obliquus: cells physicochemical properties and metabolomic profiling |
title_full | Pb(II)-phycoremediation mechanism using Scenedesmus obliquus: cells physicochemical properties and metabolomic profiling |
title_fullStr | Pb(II)-phycoremediation mechanism using Scenedesmus obliquus: cells physicochemical properties and metabolomic profiling |
title_full_unstemmed | Pb(II)-phycoremediation mechanism using Scenedesmus obliquus: cells physicochemical properties and metabolomic profiling |
title_short | Pb(II)-phycoremediation mechanism using Scenedesmus obliquus: cells physicochemical properties and metabolomic profiling |
title_sort | pb(ii)-phycoremediation mechanism using scenedesmus obliquus: cells physicochemical properties and metabolomic profiling |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8866896/ https://www.ncbi.nlm.nih.gov/pubmed/35243087 http://dx.doi.org/10.1016/j.heliyon.2022.e08967 |
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